Manipulations with early mouse embryos for generation of genetically modified animals
Вавиловский журнал генетики и селекции, 2017 Recently, genome-editing technologies have become more efficient and accessible. The discovery of nucleases for directional genome editing (CRISPR/Cas9, TALEN, ZFNs) significantly accelerated and simplified the production of mice with targeted gene ...
A. N. Korablev +2 more
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Efficient DNA knock-in using AAV-mediated delivery with 2-cell embryo CRISPR-Cas9 electroporation
Frontiers in Genome Editing, 2023 Recent advances in CRISPR-Cas genome editing technology have been instrumental in improving the efficiency to produce genetically modified animal models.
Daniel J. Davis +6 more
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Genetic platelet depletion is superior in platelet transfusion compared to current models
Haematologica, 2020 Genetically modified mice have advanced our knowledge on platelets in hemostasis and beyond tremendously. However, mouse models harbor certain limitations, including availability of platelet specific transgenic strains, and off-target effects on other ...
Manuel Salzmann +11 more
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Mapping genetic modifiers of survival in a mouse model of Dravet syndrome [PDF]
Genes, Brain and Behavior, 2013 Epilepsy is a common neurological disorder affecting approximately 1% of the population. Mutations in voltage‐gated sodium channels are responsible for several monogenic epilepsy syndromes. More than 800 mutations in the voltage‐gated sodium channel SCN1A have been reported in patients with generalized epilepsy with febrile seizures plus and Dravet ...
Jennifer A. Kearney +3 more
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An open-source video tracking system for mouse locomotor activity analysis
BMC Research Notes, 2020 Objective The ability to accurately and efficiently quantify mouse locomotor activity is essential for evaluating therapeutic efficacy and phenotyping genetically modified mice, in particular for the research of neuromuscular diseases.
Chen Zhang, Haiwen Li, Renzhi Han
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Cancer immunoprevention: from mice to early clinical trials
BMC Immunology, 2018 Cancer immunoprevention is based on the fact that a functioning immune system controls tumor onset and development in humans and animals, thus leading to the idea that the enhancement of immune responses in healthy individuals could effectively reduce ...
Arianna Palladini +3 more
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Blood-testis barrier and spermatogenesis: lessons from genetically-modified mice
Asian Journal of Andrology, 2014 The blood-testis barrier (BTB) is found between adjacent Sertoli cells in the testis where it creates a unique microenvironment for the development and maturation of meiotic and postmeiotic germ cells in seminiferous tubes.
Xiao-Hua Jiang +6 more
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sgRNA Knock-in Mouse Provides an Alternative Approach for In Vivo Genetic Modification
Frontiers in Cell and Developmental Biology, 2022 Functional genomics in a mammalian model such as mice is fundamental for understanding human biology. The CRISPR/Cas9 system dramatically changed the tempo of obtaining genetic mouse models due to high efficiency.
Lichen Zhang +25 more
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Hotspots of dendritic spine turnover facilitate clustered spine addition and learning and memory
Nature Communications, 2018 Structural remodeling of dendritic spines is thought to be a mechanism of memory storage. Here, the authors look at how spine turnover and clustering predict future learning and memory performance, and see that a genetically modified mouse with enhanced ...
Adam C. Frank +10 more
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Maternal Supply of Cas9 to Zygotes Facilitates the Efficient Generation of Site-Specific Mutant Mouse Models. [PDF]
PLoS ONE, 2017 Genome manipulation in the mouse via microinjection of CRISPR/Cas9 site-specific nucleases has allowed the production time for genetically modified mouse models to be significantly reduced.
Alberto Cebrian-Serrano +5 more
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