Results 21 to 30 of about 516,597 (217)

High-throughput screening of human genetic variants by pooled prime editing

Cell Genomics
Summary: Multiplexed assays of variant effect (MAVEs) enable scalable functional assessment of human genetic variants. However, established MAVEs are limited by exogenous expression of variants or constraints of genome editing.
Michael Herger, Christina M. Kajba, Megan Buckley, Ana Cunha, Molly Strom, Gregory M. Findlay   +5 more
doaj   +1 more source

Functional RNA Elements in the Dengue Virus Genome

Viruses, 2011
Dengue virus (DENV) genome amplification is a process that involves the viral RNA, cellular and viral proteins, and a complex architecture of cellular membranes.
Andrea V. Gamarnik, Claudia V. Filomatori, Leopoldo G. Gebhard   +2 more
doaj   +1 more source

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

Dengue Virus Genome Uncoating Requires Ubiquitination

mBio, 2016
The process of genome release or uncoating after viral entry is one of the least-studied steps in the flavivirus life cycle. Flaviviruses are mainly arthropod-borne viruses, including emerging and reemerging pathogens such as dengue, Zika, and West Nile ...
Laura A. Byk, Néstor G. Iglesias, Federico A. De Maio, Leopoldo G. Gebhard, Mario Rossi, Andrea V. Gamarnik   +5 more
doaj   +1 more source

Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathy

Nature Communications, 2023
Dilated cardiomyopathy is the second most common cause for heart failure with no cure except a high-risk heart transplantation. Approximately 30% of patients harbor heritable mutations which are amenable to CRISPR-based gene therapy.
Markus Grosch, Laura Schraft, Adrian Chan, Leonie Küchenhoff, Kleopatra Rapti, Anne-Maud Ferreira, Julia Kornienko, Shengdi Li, Michael H. Radke, Chiara Krämer, Sandra Clauder-Münster, Emerald Perlas, Johannes Backs, Michael Gotthardt, Christoph Dieterich, Maarten M. G. van den Hoogenhof, Dirk Grimm, Lars M. Steinmetz   +17 more
doaj   +1 more source

Epigenetic blind spots – the role of DNA methylation dynamics in stem cell‐based models of embryogenesis

FEBS Letters, EarlyView.
Embryo‐like structures (stembryos) are an innovative tool, but they are hindered by experimental variability and limited developmental potential. DNA methylation is crucial for mammalian development, but its status in stembryo models is poorly characterized.
Sara Canil, Anna Corrà, Annalisa Scapolatiello, Chengxiang Qiu, Gianluca Amadei   +4 more
wiley   +1 more source

Virseqimprover: an integrated pipeline for viral contig error correction, extension, and annotation [PDF]

PeerJ
Despite the recent surge of viral metagenomic studies, it remains a significant challenge to recover complete virus genomes from metagenomic data. The majority of viral contigs generated from de novo assembly programs are highly fragmented, presenting ...
Haoqiu Song, Saima Sultana Tithi, Connor Brown, Frank O. Aylward, Roderick Jensen, Liqing Zhang   +5 more
doaj   +2 more sources

Human Cytomegalovirus Genomes Survive Mitosis via the IE19 Chromatin-Tethering Domain

mBio, 2020
The genomes of DNA tumor viruses regain nuclear localization after nuclear envelope breakdown during mitosis through the action of a viral protein with a chromatin-tethering domain (CTD).
Shelby M. Lyon, Kristen D. Yetming, Christina Paulus, Michael Nevels, Robert F. Kalejta   +4 more
doaj   +1 more source

Biophysical approaches for studying viral entry

FEBS Letters, EarlyView.
Viruses infect all living organisms and have been responsible for major epidemics and pandemics. Their ongoing evolutionary battle with host defenses creates a constant need for improved tools to study viral behavior. Advancing methods to probe viral attachment, fusion, and genome release deepen our understanding of how infections begin and support the
Inbar Yosibash, Raya Sorkin
wiley   +1 more source

Chemistry and Bioinformatics Considerations in Using Next-Generation Sequencing Technologies to Inferring HIV Proviral DNA Genome-Intactness

Viruses, 2021
HIV persists via integration of the viral DNA into the human genome. The HIV DNA pool within an infected individual is a complex population that comprises both intact and defective viral genomes, each with a distinct integration site, in addition to a ...
Guinevere Q. Lee
doaj   +1 more source