Results 1 to 10 of about 1,550,449 (243)
Systematic comparison of high-throughput single-cell RNA-seq methods for immune cell profiling
BMC Genomics, 2021 Background Elucidation of immune populations with single-cell RNA-seq has greatly benefited the field of immunology by deepening the characterization of immune heterogeneity and leading to the discovery of new subtypes.
Tracy M. Yamawaki +10 more
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Wnt signaling related transcripts and their relationship to energy metabolism in C2C12 myoblasts under temperature stress [PDF]
PeerJ, 2021 Temperature stress is one of the main environmental stressors affecting the welfare, health and productivity of livestock. Temperature changes can modify cell membrane components, disrupting the crosstalk between the cell and its surroundings by ...
Marua Abu Risha +7 more
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BMC Genomics, 2021 Background Archival formalin-fixed, paraffin-embedded (FFPE) tissue samples with clinical and histological data are a singularly valuable resource for developing new molecular biomarkers.
Jin Sung Jang +7 more
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Pooled Screens Identify GPR108 and TM9SF2 as Host Cell Factors Critical for AAV Transduction
Molecular Therapy: Methods & Clinical Development, 2020 Adeno-associated virus (AAV) has been used extensively as a vector for gene therapy. Despite its widespread use, the mechanisms by which AAV enters the cell and is trafficked to the nucleus are poorly understood.
W. Hans Meisen +8 more
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Molecular Therapy: Methods & Clinical Development, 2020 Recombinant adeno-associated virus (rAAV) vectors are a leading gene delivery platform, but vector manufacturing remains a challenge. New methods are needed to increase rAAV yields and reduce costs. Past efforts to improve rAAV production have focused on
Huiren Zhao +8 more
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Chamber-enriched gene expression profiles in failing human hearts with reduced ejection fraction
Scientific Reports, 2021 Heart failure with reduced ejection fraction (HFrEF) constitutes 50% of HF hospitalizations and is characterized by high rates of mortality. To explore the underlying mechanisms of HFrEF etiology and progression, we studied the molecular and cellular ...
Xin Luo +12 more
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BMC Cancer, 2021 Background Triple negative breast cancer (TNBC) is the most aggressive subtype of breast cancer (BC). Treatment options for TNBC patients are limited and further insights into disease aetiology are needed to develop better therapeutic approaches ...
Chiara Giacomelli +12 more
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npj Breast Cancer, 2023 Intratumoral heterogeneity impacts the success or failure of anti-cancer therapies. Here, we investigated the evolution and mechanistic heterogeneity in clonal populations of cell models for estrogen receptor positive breast cancer.
Lukas Beumers +14 more
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Characterization of RNA in exosomes secreted by human breast cancer cell lines using next-generation sequencing [PDF]
PeerJ, 2013 Exosomes are nanosized (30–100 nm) membrane vesicles secreted by most cell types. Exosomes have been found to contain various RNA species including miRNA, mRNA and long non-protein coding RNAs. A number of cancer cells produce elevated levels of exosomes.
Piroon Jenjaroenpun +5 more
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Sequencing quality assessment tools to enable data-driven informatics for high throughput genomics
Frontiers in Genetics, 2013 The processes of quality assessment and control are an active area of research at The Genome Analysis Centre (TGAC). Unlike other sequencing centres that often concentrate on a certain species or technology, TGAC applies expertise in genomics and ...
Richard Mark Leggett +4 more
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