Results 1 to 10 of about 429,102 (229)
Molecular Biotechnology, 2022 Recent advances in genome editing technologies have redefined our ability to probe and precisely edit the human genome and epigenome in vitro and in vivo. More specifically, RNA-guided CRISPR/Cas systems have revolutionized the field due to their simplicity in design and adaptability across biological systems.
Maura McGrail +2 more
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N 1-methyl-pseudouridine is incorporated with higher fidelity than pseudouridine in synthetic RNAs
Scientific Reports, 2022 In vitro transcribed synthetic messenger RNAs (mRNAs) represent a novel therapeutic modality. To overcome the inherent immunogenicity, as well as to increase the therapeutic efficacy of the molecules, uridine analogs—such as pseudouridine (Ψ) and N 1 ...
Tien-Hao Chen +4 more
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System analysis of the sequencing quality of human whole exome samples on BGI NGS platform
Scientific Reports, 2022 Human exome sequencing is a classical method used in most medical genetic applications. The leaders in the field are the manufacturers of enrichment kits based on hybridization of cRNA or cDNA biotinylated probes specific for a genomic region of interest.
Vera Belova +10 more
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Deep sampling of gRNA in the human genome and deep-learning-informed prediction of gRNA activities
Cell Discovery, 2023 Life science studies involving clustered regularly interspaced short palindromic repeat (CRISPR) editing generally apply the best-performing guide RNA (gRNA) for a gene of interest.
Heng Zhang +8 more
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Genetically encoded phosphatidylserine biosensor for in vitro, ex vivo and in vivo labelling
Cellular & Molecular Biology Letters, 2023 Background The dynamics of phosphatidylserine in the plasma membrane is a tightly regulated feature of eukaryotic cells. Phosphatidylserine (PS) is found preferentially in the inner leaflet of the plasma membrane.
Eimina Dirvelyte +7 more
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BMC Genomics, 2022 Exome sequencing is becoming a routine in health care, because it increases the chance of pinpointing the genetic cause of an individual patient's condition and thus making an accurate diagnosis.
Vera Belova +8 more
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PEAC-seq adopts Prime Editor to detect CRISPR off-target and DNA translocation
Nature Communications, 2022 It is still a challenge to accurately identify off-target endonuclease edits. Here the authors report PEAC-seq using a Prime Editor to insert a tag to the editing sites and enrich the tagged regions with site-specific primers for sequencing: they show ...
Zhenxing Yu +10 more
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BMC Plant Biology, 2021 Background Virus-induced gene silencing (VIGS) is one of the most convenient and powerful methods of reverse genetics. In vitro-inoculation of plant virus is an important method for studying the interactions between viruses and plants.
Qili Liu +16 more
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Transcription shapes DNA replication initiation to preserve genome integrity
Genome Biology, 2021 Background Early DNA replication occurs within actively transcribed chromatin compartments in mammalian cells, raising the immediate question of how early DNA replication coordinates with transcription to avoid collisions and DNA damage.
Yang Liu +10 more
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Peroxiredoxins—The Underrated Actors during Virus-Induced Oxidative Stress
Antioxidants, 2021 Enhanced production of reactive oxygen species (ROS) triggered by various stimuli, including viral infections, has attributed much attention in the past years. It has been shown that different viruses that cause acute or chronic diseases induce oxidative
Inna L. Karpenko +4 more
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