Results 11 to 20 of about 622,112 (341)

A rapid, simple, and highly efficient method for VIGS and in vitro-inoculation of plant virus by INABS applied to crops that develop axillary buds and can survive from cuttings

BMC Plant Biology, 2021
Background Virus-induced gene silencing (VIGS) is one of the most convenient and powerful methods of reverse genetics. In vitro-inoculation of plant virus is an important method for studying the interactions between viruses and plants.
Qili Liu, Kedong Xu, Lun Yi, Yalin Hou, Dongxiao Li, Haiyan Hu, Feng Zhou, Puwen Song, Yongang Yu, Qichao Wei, Yuanyuan Guan, Ping Hu, Ruifang Bu, Eryong Chen, Xiaojia Su, Honglian Li, Chengwei Li   +16 more
doaj   +1 more source

BATCH-GE : batch analysis of next-generation sequencing data for genome editing assessment [PDF]

, 2016
Targeted mutagenesis by the CRISPR/Cas9 system is currently revolutionizing genetics. The ease of this technique has enabled genome engineering in-vitro and in a range of model organisms and has pushed experimental dimensions to unprecedented proportions.
Boel, Annekatrien, Callewaert, Bert, Coucke, Paul, De Paepe, Anne, De Rocker, Nina, Menten, Björn, Steyaert, Wouter, Willaert, Andy   +7 more
core   +2 more sources

Give CRISPR a chance : the GeneSprout Initiative [PDF]

, 2020
Did you know that a group of early-career researchers launched an initiative enabling open dialog on new plant breeding techniques, such as genome editing?
Boer, Damian, de Koning, Ramon, Houben, Maarten, Huybrechts, Michiel, Meyer, Prisca, Rienstra, Juriaan, Sajeev, Nikita, Swinnen, Gwen, Vangheluwe, Nick   +8 more
core   +2 more sources

Transcription shapes DNA replication initiation to preserve genome integrity

Genome Biology, 2021
Background Early DNA replication occurs within actively transcribed chromatin compartments in mammalian cells, raising the immediate question of how early DNA replication coordinates with transcription to avoid collisions and DNA damage.
Yang Liu, Chen Ai, Tingting Gan, Jinchun Wu, Yongpeng Jiang, Xuhao Liu, Rusen Lu, Ning Gao, Qing Li, Xiong Ji, Jiazhi Hu   +10 more
doaj   +1 more source

Peroxiredoxins—The Underrated Actors during Virus-Induced Oxidative Stress

Antioxidants, 2021
Enhanced production of reactive oxygen species (ROS) triggered by various stimuli, including viral infections, has attributed much attention in the past years. It has been shown that different viruses that cause acute or chronic diseases induce oxidative
Inna L. Karpenko, Vladimir T. Valuev-Elliston, Olga N. Ivanova, Olga A. Smirnova, Alexander V. Ivanov   +4 more
doaj   +1 more source

Gene editing CRISPR/Cas9 system for producing cows with hypoallergenic milk on the background of a beta-lactoglobulin gene knockout [PDF]

E3S Web of Conferences, 2020
Beta-lactoglobulin is the main allergen of cow’s milk. Modern approaches to reducing the allergenicity of milk require significant costs for its fermentation. An alternative approach could be the creation of productive breeds with a knockout for the gene
Silaeva Yulia Yu., Kubekina Marina V., Bruter Alexandra V., Isaeva Albina G., Koshchaev Andrey G.   +4 more
doaj   +1 more source

EGR1 and RXRA transcription factors link TGF-β pathway and CCL2 expression in triple negative breast cancer cells

Scientific Reports, 2021
Transforming growth factor beta (TGF-β) is the main cytokine responsible for the induction of the epithelial-mesenchymal transition of breast cancer cells, which is a hallmark of tumor transformation to the metastatic phenotype.
Alisa M. Gorbacheva, Aksinya N. Uvarova, Alina S. Ustiugova, Arindam Bhattacharyya, Kirill V. Korneev, Dmitry V. Kuprash, Nikita A. Mitkin   +6 more
doaj   +1 more source

DNA editing in DNA/RNA hybrids by adenosine deaminases that act on RNA. [PDF]

, 2017
Adenosine deaminases that act on RNA (ADARs) carry out adenosine (A) to inosine (I) editing reactions with a known requirement for duplex RNA. Here, we show that ADARs also react with DNA/RNA hybrid duplexes.
Beal, Peter A, Lorenzo, Claire, Zheng, Yuxuan   +2 more
core   +1 more source

Precision genome editing [PDF]

Nature Methods, 2018
A machine learning model predicts the genotype of CRISPR–Cas9 gene editing products, thereby enabling precise, template-free correction of disease-associated mutations.
openaire   +2 more sources

Defect in cytosolic Neu2 sialidase abrogates lipid metabolism and impairs muscle function in vivo

Scientific Reports, 2022
Sialic acid (SA) is present in glycoconjugates and important in cell–cell recognition, cell adhesion, and cell growth and as a receptor. Among the four mammalian sialidases, cytosolic NEU2 has a pivotal role in muscle and neuronal differentiation in ...
Mijung Oh, Dae-In Ha, Chaeyeon Son, Jeong Gu Kang, Heeyoun Hwang, Su Bin Moon, Minjeong Kim, Jihae Nam, Jung Soo Kim, Sang Yong Song, Yong-Sam Kim, Sangwoo Park, Jong Shin Yoo, Jeong-Heon Ko, Kyoungsook Park   +14 more
doaj   +1 more source