Results 21 to 30 of about 1,792,457 (385)
Strategies in the delivery of Cas9 ribonucleoprotein for CRISPR/Cas9 genome editing
Theranostics, 2021 CRISPR/Cas9 genome editing has gained rapidly increasing attentions in recent years, however, the translation of this biotechnology into therapy has been hindered by efficient delivery of CRISPR/Cas9 materials into target cells. Direct delivery of CRISPR/
Song Zhang +3 more
semanticscholar +1 more source
De Novo Transcriptome Profiling of Brain Tissue from the Annual Killifish Nothobranchius guentheri
Life, 2021 Nothobranchius is a genus of small annual killifish found in Africa. Due to the relatively short lifespan, as well as easy breeding and care, Nothobranchius fish are becoming widely used as a vertebrate model system. Studying the genome and transcriptome
Zulfiia G. Guvatova +14 more
doaj +1 more source
CRISPR SWAPnDROP -- A multifunctional system for genome editing and large-scale interspecies gene transfer [PDF]
, 2021 The need for diverse chromosomal modifications in biotechnology, synthetic biology and basic research requires the development of new technologies. With CRISPR SWAPnDROP, we extend the limits of genome editing to large-scale in-vivo DNA transfer between bacterial species.
arxiv +1 more source
Defect in cytosolic Neu2 sialidase abrogates lipid metabolism and impairs muscle function in vivo
Scientific Reports, 2022 Sialic acid (SA) is present in glycoconjugates and important in cell–cell recognition, cell adhesion, and cell growth and as a receptor. Among the four mammalian sialidases, cytosolic NEU2 has a pivotal role in muscle and neuronal differentiation in ...
Mijung Oh +14 more
doaj +1 more source
New PAM Improves the Single-Base Specificity of crRNA-Guided LbCas12a Nuclease
Life, 2022 The RNA-guided Cas12a nuclease forms a complex with a CRISPR RNA (crRNA) to cleave the double-stranded DNA target. Among others, Cas12a protein from Lachnospiraceae bacterium (LbCas12a) is widely used for biomedical research.
Mariia A. Misiurina +5 more
doaj +1 more source
THE PROMISE AND CHALLENGE OF THERAPEUTIC GENOME EDITING
Nature, 2020 Genome editing, which involves the precise manipulation of cellular DNA sequences to alter cell fates and organism traits, has the potential to both improve our understanding of human genetics and cure genetic disease.
J. Doudna
semanticscholar +1 more source
Proceedings of the National Academy of Sciences of the United States of America, 2021 Significance Genome editing technologies enable the permanent repair of disease-causing genetic mutations. However, the application of this technology has been limited by the technical challenge of achieving safe, effective, and specific in vivo delivery
Min Qiu +10 more
semanticscholar +1 more source
Mitochondrial DNA. Part B. Resources, 2020 The family Provannidae is endemic to chemosynthetic environments in the deep-sea. Here, we report the complete mitogenome of a provannid vent snail Alviniconcha boucheti from the North Fiji Basin for the first time. The length of mitogenome was 15,981 bp,
Won-Kyung Lee +3 more
doaj +1 more source
Cas9 exo-endonuclease eliminates chromosomal translocations during genome editing
Nature Communications, 2022 Chromosomal structural variations induced by CRISPR/Cas hinder its application in clinics. Here, the authors fuse Cas9 with optimized TREX2 to generate Cas9TX, which can prevent perfect repair and inhibit repeated cleavage.
Jianhang Yin +15 more
doaj +1 more source
Chromothripsis as an on-target consequence of CRISPR-Cas9 genome editing
Nature Genetics, 2020 Genome editing has therapeutic potential for treating genetic diseases and cancer. However, the currently most practicable approaches rely on the generation of DNA double-strand breaks (DSBs), which can give rise to a poorly characterized spectrum of ...
Mitchell L. Leibowitz +8 more
semanticscholar +1 more source