Practical guidance for the implementation of the CRISPR genome editing tool in filamentous fungi [PDF]
, 2019 Background: Within the last years, numerous reports described successful application of the CRISPR nucleases Cas9 and Cpf1 for genome editing in filamentous fungi.
Haefner, Stefan +4 more
core +1 more source
CRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding [PDF]
, 2020 Open Access Journal; Published online: 31 Jan 2019Presence of the integrated endogenous banana streak virus (eBSV) in the B genome of plantain (AAB) is a major challenge for breeding and dissemination of hybrids.
Britt, A. +5 more
core +1 more source
GENT2: an updated gene expression database for normal and tumor tissues
BMC Medical Genomics, 2019 Background Gene Expression database of Normal and Tumor tissues 2 (GENT2) is an updated version of GENT, which has provided a user-friendly search platform for gene expression patterns across different normal and tumor tissues compiled from public gene ...
Seung-Jin Park +3 more
doaj +1 more source
Genome editing in potato via CRISPR-Cas9 ribonucleoprotein delivery [PDF]
, 2018 Clustered regularly interspaced short palindromic repeats and CRISPR-associated protein-9 (CRISPR-Cas9) can be used as an efficient tool for genome editing in potato (Solanum tuberosum).
Andersson +22 more
core +1 more source
The hallmarks of dietary intervention-resilient gut microbiome
npj Biofilms and Microbiomes, 2022 Maintaining equilibrium of the gut microbiome is crucial for human health. Diet represents an important and generally accessible natural channel of controlling the nutrients supply to the intestinal microorganisms.
Natalia S. Klimenko +3 more
doaj +1 more source
Improving single-cell cloning workflow for gene editing in human pluripotent stem cells [PDF]
, 2018 The availability of human pluripotent stem cells (hPSCs) and progress in genome engineering technology have altered the way we approach scientific research and drug development screens.
Chen, Yi-Hsien, Pruett-Miller, Shondra M
core +2 more sources
Antibiotic Resistance in Neisseria gonorrhoeae: Challenges in Research and Treatment
Microorganisms, 2022 Gonococcal infection caused by the Gram-negative bacteria Neisseria gonorrhoeae is one of the most common sexually transmitted infections (STIs) worldwide [...]
Boris Shaskolskiy +3 more
doaj +1 more source
Population and allelic variation of A-to-I RNA editing in human transcriptomes. [PDF]
, 2017 BackgroundA-to-I RNA editing is an important step in RNA processing in which specific adenosines in some RNA molecules are post-transcriptionally modified to inosines.
Demirdjian, Levon +6 more
core +2 more sources
Improved genome editing in human cell lines using the CRISPR method [PDF]
, 2014 The Cas9/CRISPR system has become a popular choice for genome editing. In this system, binding of a single guide (sg) RNA to a cognate genomic sequence enables the Cas9 nuclease to induce a double-strand break at that locus.
AW Briggs +22 more
core +9 more sources
Switchable genome editing via genetic code expansion [PDF]
, 2018 Multiple applications of genome editing by CRISPR-Cas9 necessitate stringent regulation and Cas9 variants have accordingly been generated whose activity responds to small ligands, temperature or light.
Asami, Maki +5 more
core +2 more sources