Results 31 to 40 of about 1,632,775 (273)
Defect in cytosolic Neu2 sialidase abrogates lipid metabolism and impairs muscle function in vivo
Scientific Reports, 2022 Sialic acid (SA) is present in glycoconjugates and important in cell–cell recognition, cell adhesion, and cell growth and as a receptor. Among the four mammalian sialidases, cytosolic NEU2 has a pivotal role in muscle and neuronal differentiation in ...
Mijung Oh +14 more
doaj +1 more source
New PAM Improves the Single-Base Specificity of crRNA-Guided LbCas12a Nuclease
Life, 2022 The RNA-guided Cas12a nuclease forms a complex with a CRISPR RNA (crRNA) to cleave the double-stranded DNA target. Among others, Cas12a protein from Lachnospiraceae bacterium (LbCas12a) is widely used for biomedical research.
Mariia A. Misiurina +5 more
doaj +1 more source
THE PROMISE AND CHALLENGE OF THERAPEUTIC GENOME EDITING
Nature, 2020 Genome editing, which involves the precise manipulation of cellular DNA sequences to alter cell fates and organism traits, has the potential to both improve our understanding of human genetics and cure genetic disease.
J. Doudna
semanticscholar +1 more source
Mitochondrial DNA. Part B. Resources, 2020 The family Provannidae is endemic to chemosynthetic environments in the deep-sea. Here, we report the complete mitogenome of a provannid vent snail Alviniconcha boucheti from the North Fiji Basin for the first time. The length of mitogenome was 15,981 bp,
Won-Kyung Lee +3 more
doaj +1 more source
The Future of Genome Editing [PDF]
Cell, 2018 The power of CRISPR is undeniable, but where is the field heading? Cell's April Pawluk caught up with Jia Chen, Weizhi Ji, and Prashant Mali to discuss the successes and challenges we can expect in the coming years. Annotated excerpts from this conversation are presented below, and the full conversation is available with the article online.
Jia Chen +3 more
openaire +3 more sources
DNA editing in DNA/RNA hybrids by adenosine deaminases that act on RNA. [PDF]
, 2017 Adenosine deaminases that act on RNA (ADARs) carry out adenosine (A) to inosine (I) editing reactions with a known requirement for duplex RNA. Here, we show that ADARs also react with DNA/RNA hybrid duplexes.
Beal, Peter A +2 more
core +1 more source
Highly efficient genome editing by CRISPR-Cpf1 using CRISPR RNA with a uridinylate-rich 3′-overhang
Nature Communications, 2018 Cpf1 is a promising alternative to Cas9 though indel generation efficiency is target dependent. Here the authors show that the addition of a polyU 3′ overhang can improve the efficiency of low efficiency guide RNAs.
Su Bin Moon +10 more
doaj +1 more source
Cas9 exo-endonuclease eliminates chromosomal translocations during genome editing
Nature Communications, 2022 Chromosomal structural variations induced by CRISPR/Cas hinder its application in clinics. Here, the authors fuse Cas9 with optimized TREX2 to generate Cas9TX, which can prevent perfect repair and inhibit repeated cleavage.
Jianhang Yin +15 more
doaj +1 more source
Nature Communications, 2020 Artificially improving traits of cultivated alfalfa (Medicago sativa L.), one of the most important forage crops, is challenging due to the lack of a reference genome and an efficient genome editing protocol, which mainly result from its autotetraploidy ...
Haitao Chen +23 more
semanticscholar +1 more source
Improving single-cell cloning workflow for gene editing in human pluripotent stem cells [PDF]
, 2018 The availability of human pluripotent stem cells (hPSCs) and progress in genome engineering technology have altered the way we approach scientific research and drug development screens.
Chen, Yi-Hsien, Pruett-Miller, Shondra M
core +2 more sources