Results 31 to 40 of about 1,792,457 (385)
Mechanism and Applications of CRISPR/Cas-9-Mediated Genome Editing
Biologics : targets & therapy, 2021 Clustered regularly interspaced short palindromic repeat (CRISPR) and their associated protein (Cas-9) is the most effective, efficient, and accurate method of genome editing tool in all living cells and utilized in many applied disciplines.
Misganaw Asmamaw, Belay Zawdie
semanticscholar +1 more source
Highly efficient genome editing by CRISPR-Cpf1 using CRISPR RNA with a uridinylate-rich 3′-overhang
Nature Communications, 2018 Cpf1 is a promising alternative to Cas9 though indel generation efficiency is target dependent. Here the authors show that the addition of a polyU 3′ overhang can improve the efficiency of low efficiency guide RNAs.
Su Bin Moon +10 more
doaj +1 more source
Large-Scale CRISPR Screen of LDLR Pathogenic Variants
Research, 2023 Familial hypercholesterolemia (FH) is a frequently occurring genetic disorder that is linked to early-onset cardiovascular disease. If left untreated, patients with this condition can develop severe cardiovascular complications.
Mengjing Li +9 more
doaj +1 more source
Evolution of genome size in asexual populations [PDF]
Scientific Reports 6 (2016) 25786, 2015 Genome sizes have evolved to vary widely, from 250 bases in viroids to 670 billion bases in amoeba. This remarkable variation in genome size is the outcome of complex interactions between various evolutionary factors such as point mutation rate, population size, insertions and deletions, and genome editing mechanisms that may be specific to certain ...
arxiv +1 more source
CRISPR-Cas9 genome editing using targeted lipid nanoparticles for cancer therapy
Science Advances, 2020 CRISPR lipid nanoparticles promote in vivo therapeutic genome editing in two aggressive cancer mouse models. Harnessing CRISPR-Cas9 technology for cancer therapeutics has been hampered by low editing efficiency in tumors and potential toxicity of ...
Daniel Rosenblum +11 more
semanticscholar +1 more source
Challenges Facing CRISPR/Cas9-Based Genome Editing in Plants
Frontiers in Plant Science, 2022 The development of plant varieties with desired traits is imperative to ensure future food security. The revolution of genome editing technologies based on the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated nuclease ...
Seungmin Son, Sang Ryeol Park
semanticscholar +1 more source
The hallmarks of dietary intervention-resilient gut microbiome
npj Biofilms and Microbiomes, 2022 Maintaining equilibrium of the gut microbiome is crucial for human health. Diet represents an important and generally accessible natural channel of controlling the nutrients supply to the intestinal microorganisms.
Natalia S. Klimenko +3 more
doaj +1 more source
In vivo genome editing using Staphylococcus aureus Cas9
Nature, 2015 The RNA-guided endonuclease Cas9 has emerged as a versatile genome-editing platform. However, the size of the commonly used Cas9 from Streptococcus pyogenes (SpCas9) limits its utility for basic research and therapeutic applications that use the highly ...
F. Ran +12 more
semanticscholar +1 more source
Genome editing around the globe: An update on policies and perceptions
Plant Physiology, 2022 A decade ago, the CRISPR/Cas system has been adapted for genome editing. Since then, hundreds of organisms have been altered using genome editing and discussions were raised on the regulatory status of genome edited organisms esp. crops.
T. Sprink, R. Wilhelm, F. Hartung
semanticscholar +1 more source
The Future of Genome Editing [PDF]
Cell, 2018 The power of CRISPR is undeniable, but where is the field heading? Cell's April Pawluk caught up with Jia Chen, Weizhi Ji, and Prashant Mali to discuss the successes and challenges we can expect in the coming years. Annotated excerpts from this conversation are presented below, and the full conversation is available with the article online.
Jia Chen +3 more
openaire +3 more sources