Results 51 to 60 of about 1,792,457 (385)
Attention-based Multi-task Learning for Base Editor Outcome Prediction [PDF]
arXiv, 2023 Human genetic diseases often arise from point mutations, emphasizing the critical need for precise genome editing techniques. Among these, base editing stands out as it allows targeted alterations at the single nucleotide level. However, its clinical application is hindered by low editing efficiency and unintended mutations, necessitating extensive ...
arxiv +1 more source
Nature Communications, 2020 CRISPR-Cas9 has emerged as a powerful technology that relies on Cas9/sgRNA ribonucleoprotein complexes (RNPs) to target and edit DNA. However, many therapeutic targets cannot currently be accessed due to the lack of carriers that can deliver RNPs ...
Tuo Wei +4 more
semanticscholar +1 more source
Frontiers in Immunology, 2023 Regulatory B lymphocytes (Bregs) are B cells with well-pronounced immunosuppressive properties, allowing them to suppress the activity of effector cells.
Elina A. Zheremyan +11 more
doaj +1 more source
Prokaryotic genome editing based on the subtype I-B-Svi CRISPR-Cas system [PDF]
arXiv, 2023 Type I CRISPR-Cas systems are the most common among six types of CRISPR-Cas systems, however, non-self-targeting genome editing based on a single Cas3 of type I CRISPR-Cas systems has not been reported. Here, we present the subtype I-B-Svi CRISPR-Cas system (with three confirmed CRISPRs and a cas gene cluster) and genome editing based on this system ...
arxiv
Journal of Extracellular Vesicles, 2021 Transient delivery of CRISPR‐based genome editing effectors is important to reduce off‐target effects and immune responses. Recently extracellular vesicles (EVs) have been explored for Cas9 ribonucleoprotein (RNP) delivery. However, lack of mechanisms to
Xingang Yao +6 more
semanticscholar +1 more source
Evolutionarily conserved human targets of adenosine to inosine RNA editing [PDF]
Nucleic Acids Research, Vol. 33, 1162-1168 (2005), 2005 A-to-I RNA editing by ADARs is a post-transcriptional mechanism for expanding the proteomic repertoire. Genetic recoding by editing was so far observed for only a few mammalian RNAs that are predominantly expressed in nervous tissues. However, as these editing targets fail to explain the broad and severe phenotypes of ADAR1 knockout mice, additional ...
arxiv +1 more source
Frontiers in Genome Editing, 2022 Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) mediated genome editing is a powerful approach for crop improvement. Traditional transformation methods based on plasmid delivery pose concerns associated with transgene integration and ...
Yingxiao Zhang +8 more
semanticscholar +1 more source
Genome edited sheep and cattle [PDF]
Transgenic Research, 2014 Genome editing tools enable efficient and accurate genome manipulation. An enhanced ability to modify the genomes of livestock species could be utilized to improve disease resistance, productivity or breeding capability as well as the generation of new biomedical models. To date, with respect to the direct injection of genome editor mRNA into livestock
Proudfoot, Christopher +10 more
openaire +4 more sources
Experimental and Molecular Medicine, 2018 Stomach cancer: DNA methylation of hormone receptor genes A sequencing study reveals abnormal changes to DNA that set the stage for stomach cancer development.
Hee-Jin Kim +9 more
doaj +1 more source
Template-based eukaryotic genome editing directed by SviCas3 [PDF]
arXiv, 2023 RNA-guided gene editing based on the CRISPR-Cas system is currently the most effective genome editing technique. Here, we report that the SviCas3 from the subtype I-B-Svi Cas system in Streptomyces virginiae IBL14 is an RNA-guided and DNA-guided DNA endonuclease suitable for the HDR-directed gene and/or base editing of eukaryotic cell genomes.
arxiv