Results 31 to 40 of about 526,413 (332)

An extra dimension in protein tagging by quantifying universal proteotypic peptides using targeted proteomics [PDF]

, 2016
The use of protein tagging to facilitate detailed characterization of target proteins has not only revolutionized cell biology, but also enabled biochemical analysis through efficient recovery of the protein complexes wherein the tagged proteins reside ...
De Sutter, Delphine, Eyckerman, Sven, Gevaert, Kris, Gonnelli, Giulia, Martens, Lennart, Samyn, Noortje, Staes, An, Timmerman, Evy, Vandemoortele, Giel, Vandermarliere, Elien   +9 more
core   +2 more sources

Synthetic genome engineering gets infectious [PDF]

Proceedings of the National Academy of Sciences, 2017
Since the start of this century, a handful of research groups have pursued the synthesis and large-scale engineering of genomes. Work on synthetic genomes has seen the field scale-up from the full synthesis of the small poliovirus genome (2002) (1), to a complete working synthetic bacterial genome (2010) (2), and more recently to the construction and ...
Leslie A, Mitchell, Tom, Ellis
openaire   +2 more sources

Genome editing technologies to fight infectious diseases [PDF]

, 2017
Genome editing by programmable nucleases represents a promising tool that could be exploited to develop new therapeutic strategies to fight infectious diseases.
Barzon, Luisa, Pal\ue3\ub9, Giorgio, Trevisan, Marta   +2 more
core   +1 more source

Genome‐scale engineering for systems and synthetic biology

Molecular Systems Biology, 2013
Genome‐modification technologies enable the rational engineering and perturbation of biological systems. Historically, these methods have been limited to gene insertions or mutations at random or at a few pre‐defined locations across the genome.
Kevin M Esvelt, Harris H Wang
doaj   +1 more source

iPSCs in NK Cell Manufacturing and NKEV Development

Frontiers in Immunology, 2022
Natural killer (NK) cell immunotherapies for cancer can complement existing T cell therapies while benefiting from advancements already made in the immunotherapy field.
Nicholas Boyd-Gibbins, Peter Karagiannis, Do Won Hwang, Shin-Il Kim, Shin-Il Kim   +4 more
doaj   +1 more source

Modulating signaling networks by CRISPR/Cas9-mediated transposable element insertion [PDF]

, 2018
In a recent past, transposable elements (TEs) were referred to as selfish genetic components only capable of copying themselves with the aim of increasing the odds of being inherited.
A Bolotin, A Burt, A Hammond, AP Lorenzetti, B McClintock, BA Castilho, C Feschotte, C Feschotte, C Feschotte, C Kettlun, C Lu, C Ye, CN Hancock, DBT Cox, DJ Garfinkel, F Palazzoli, FA Ran, FJM Mojica, G Feng, G Mai, G Yang, H Kuang, H Mao, HT Dong, IK Jordan, J Hou, J Li, J Paszkowski, JA Doudna, JB Owens, JD Hollister, JE DiCarlo, JI Qüesta, JK Baillie, JM Casacuberta, K Kikuchi, K Naito, K Naito, K Shirasawa, KD Kim, KM Esvelt, KS Makarova, L Bortesi, L Cong, LC Li, LM Vaschetto, Luis María Vaschetto, M Cowley, M Jinek, M Momose, M Patel, M Szabo, M Turner, N Buchon, N Jiang, N Jiang, N Jiang, Q Zhang, R Barrangou, R Jansen, RM Walsh, SD Molyneux, SR Wessler, SR Yant, T Mourier, T Sakuma, T Singer, VM Gantz, W Matsunaga, WC Lai, X Li, X Li, X Lin, X Shan, Y Ishino, Y Yan, ZN Adelman   +76 more
core   +1 more source

Advances in yeast genome engineering [PDF]

FEMS Yeast Research, 2014
Genome engineering based on homologous recombination has been applied to yeast for many years. However, the growing importance of yeast as a cell factory in metabolic engineering and chassis in synthetic biology demands methods for fast and efficient introduction of multiple targeted changes such as gene knockouts and introduction of multistep ...
Florian, David, Verena, Siewers
openaire   +2 more sources

A Simple and Effective Method for Construction of Escherichia coli Strains Proficient for Genome Engineering [PDF]

, 2014
Multiplex genome engineering is a standalone recombineering tool for large-scale programming and accelerated evolution of cells. However, this advanced genome engineering technique has been limited to use in selected bacterial strains.
B Swingle, C Fu, C Yu, DG Yu, DL Court, FJ Isaacs, HH Wang, HH Wang, HH Wang, HH Wang, JA Mosberg, JA Mosberg, JY Jeong, K Esvelt, K Sergueev, K Sergueev, Kwangsu Shin, M Arnaud, MF Alexeyev, MG Marinus, MJ Kang, MZ Li, NR Sandoval, PD Sadowski, Rajesh Kumar Biswas, S Datta, Stefan Bereswill, Suk Min Kim, Sung Kuk Lee, T Durfee, TS Ham, V Burdett, Vinuselvi Parisutham, WW Metcalf, Y Hirayama, Young Shin Ryu   +35 more
core   +2 more sources

Non-standard amino acid incorporation into proteins using Escherichia coli cell-free protein synthesis

Frontiers in Chemistry, 2014
Incorporating non-standard amino acids (NSAAs) into proteins enables new chemical properties, new structures, and new functions. In recent years, improvements in cell-free protein synthesis (CFPS) systems have opened the way to accurate and efficient ...
Seok Hoon eHong, Yong-Chan eKwon, Michael C. Jewett   +2 more
doaj   +1 more source

Application of Gene Editing Technology in Resistance Breeding of Livestock

Life, 2022
As a new genetic engineering technology, gene editing can precisely modify the specific gene sequence of the organism’s genome. In the last 10 years, with the rapid development of gene editing technology, zinc-finger nucleases (ZFNs), transcription ...
Sutian Wang, Zixiao Qu, Qiuyan Huang, Jianfeng Zhang, Sen Lin, Yecheng Yang, Fanming Meng, Jianhao Li, Kunli Zhang   +8 more
doaj   +1 more source