Results 91 to 100 of about 1,321,379 (303)
Live genome imaging by CRISPR engineering: progress and problems
Experimental and Molecular MedicineCRISPR–Cas-based genome imaging opened a new era of genome visualization in living cells. While genomic loci with repetitive sequences, such as centromeres and telomeres, can be reliably imaged, applying the technique to nonrepetitive genomic loci has ...
Eui-Jin Park, Hajin Kim
doaj +1 more source
High-throughput targeted repeat element bisulfite sequencing (HT-TREBS): genome-wide DNA methylation analysis of IAP LTR retrotransposon. [PDF]
PLoS ONE, 2014 In vertebrates, DNA methylation-mediated repression of retrotransposons is essential for the maintenance of genomic integrity. In the current study, we developed a technique termed HT-TREBS (High-Throughput Targeted Repeat Element Bisulfite Sequencing ...
Muhammad B Ekram, Joomyeong Kim
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MITF maintains genome stability in nonmelanocyte lineages
Molecular Oncology, EarlyView.MITF is essential for melanocyte survival and acts as an oncogene in 10%–20% of melanomas. We show that MITF depletion causes genome instability in nonmelanocytic cells, leading to LATS2‐mediated P53 activation, cell cycle arrest, and apoptosis. This study highlights the role of MITF as a genome maintenance factor beyond the melanocyte lineage. Created
Drifa H. Gudmundsdottir +13 more
wiley +1 more source
Indian Journal of Ophthalmology, 2013 Contact lenses are required for the visual improvement in patients with keratoconus. Various contact lens options, such as rigid gas permeable (RGP) lenses, soft and soft toric lenses, piggy back contact lenses (PBCL), hybrid lenses and scleral lenses ...
Varsha M Rathi +2 more
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Genetics Selection Evolution, 2022 Background Genomic selection has revolutionized genetic improvement in animals and plants, but little is known about its long-term effects. Here, we investigated the long-term effects of genomic selection on response to selection, genetic variance, and ...
Yvonne C. J. Wientjes +5 more
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Quantitative assay for TALEN activity at endogenous genomic loci [PDF]
Biology Open, 2013 Summary Artificially designed nucleases such as zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) can induce a targeted DNA double-strand break at the specific target genomic locus, leading to the frameshift-mediated gene disruption.
Yu Hisano +10 more
openaire +3 more sources
Liquid biopsy‐based diagnostic evaluation of hypermethylated CpG sites for ovarian cancer diagnosis
Molecular Oncology, EarlyView.This schematic outlines the workflow from biomarker identification to duplex MethyLight assay validation for epithelial ovarian cancer diagnosis using cfDNA‐based liquid biopsy. Initial screening of hypermethylated CpG candidates (cg02957270, cg10061138 cg00480298, COL2A1) was performed in tissue using ARMS‐PCR, COBRA, qPCR and image analysis. Selected
Deepa Bisht +3 more
wiley +1 more source
Femtosecond laser enabled keratoplasty for advanced keratoconus
Indian Journal of Ophthalmology, 2013 Purpose : To assess the efficacy and advantages of femtosecond laser enabled keratoplasty (FLEK) over conventional penetrating keratoplasty (PKP) in advanced keratoconus.
Yathish Shivanna +3 more
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Cotton pan-genome retrieves the lost sequences and genes during domestication and selection
Genome Biology, 2021 Background Millennia of directional human selection has reshaped the genomic architecture of cultivated cotton relative to wild counterparts, but we have limited understanding of the selective retention and fractionation of genomic components. Results We
Jianying Li +17 more
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Molecules and Cells, 2017 Summary Pioneer transcription factors (TFs) function as genomic ‘first responders,’ binding to inaccessible regions of chromatin to promote enhancer formation.
Kraus W. Lee, Ziying Liu
semanticscholar +1 more source