Results 61 to 70 of about 1,321,379 (303)
Progress and Challenges for Live-cell Imaging of Genomic Loci Using CRISPR-based Platforms
Genomics, Proteomics & Bioinformatics, 2019 Chromatin conformation, localization, and dynamics are crucial regulators of cellular behaviors. Although fluorescence in situ hybridization-based techniques have been widely utilized for investigating chromatin architectures in healthy and diseased ...
Xiaotian Wu +4 more
semanticscholar +1 more source
NRE: a tool for exploring neutral loci in the human genome [PDF]
BMC Bioinformatics, 2012 Abstract Background Analyzing regions of the genome where genetic variation is free from the confounding effects of natural selection is essential for many population genetic studies. Several recent studies in humans have stressed the large effect of natural selection at linked neutral sites and have shown that the ...
Arbiza Leonardo +2 more
openaire +3 more sources
GigaScience, 2020 Background The development of high-throughput sequencing and genotyping methodologies has enabled the identification of thousands of genomic regions associated with several complex traits.
P. Fonseca +3 more
semanticscholar +1 more source
CRISPR/dual-FRET molecular beacon for sensitive live-cell imaging of non-repetitive genomic loci
Nucleic Acids Research, 2019 Clustered regularly interspaced short palindromic repeats (CRISPR)-based genomic imaging systems predominantly rely on fluorescent protein reporters, which lack the optical properties essential for sensitive dynamic imaging.
Shiqi Mao +4 more
semanticscholar +1 more source
FEBS Letters, EarlyView.Embryo‐like structures (stembryos) are an innovative tool, but they are hindered by experimental variability and limited developmental potential. DNA methylation is crucial for mammalian development, but its status in stembryo models is poorly characterized.
Sara Canil +4 more
wiley +1 more source
Current status of accelerated corneal cross-linking
Indian Journal of Ophthalmology, 2013 Corneal cross-linking with riboflavin is a technique to stabilize or reduce corneal ectasia, in diseases such as keratoconus and post-laser-assisted in situ keratomileusis (LASIK) ectasia.
Michael Mrochen
doaj +1 more source
Circulation Research, 2016 Genome-wide association studies have provided a rich collection of ≈58 coronary artery disease (CAD) loci that suggest the existence of previously unsuspected new biology relevant to atherosclerosis. However, these studies only identify genomic loci associated with CAD, and many questions remain even after a genomic locus is definitively implicated ...
Sylvia T, Nurnberg +6 more
openaire +2 more sources
Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis
FEBS Letters, EarlyView.Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee +7 more
wiley +1 more source
Investigating transcription factor dynamics in health and disease using FRAP
FEBS Letters, EarlyView.FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj +3 more
wiley +1 more source
Topography-guided custom ablation treatment for treatment of keratoconus
Indian Journal of Ophthalmology, 2013 Keratoconus is a progressive ectatic disorder of the cornea which often presents with fluctuating refraction and high irregular astigmatism. Correcting the vision of these patients is often a challenge because glasses are unable to correct the irregular ...
Rohit Shetty +3 more
doaj +1 more source