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Genotyping-by-Sequencing in Potato
, 2017Genotyping-by-sequencing (GBS) is one of the most cost-effective approaches to sequencing in potato (SNP) discovery and genotyping. The reduction of genome complexity that is central to the GBS approach is useful in the analysis of many plant genomes in which large size and polyploidy can prove challenging.
Chiheb Boudhrioua +6 more
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Genotyping-by-Sequencing Technology in Plant Taxonomy and Phylogeny [PDF]
, 2020 Genotyping-by-sequencing (GBS) is a method to discover and genotype simultaneous genome-wide high-throughput single nucleotide polymorphisms (SNPs). GBS is based on reducing genome complexity with restriction enzymes. Here we describe a method developed by Elshire et al.
Favre, Félicien +3 more
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Advances of genotyping-by-sequencing in fisheries and aquaculture
Reviews in Fish Biology and Fisheries, 2017The use of genotyping has enabled the characterization and mapping of genes and the study of stock identification, population genetics, evolution, ecological speciation, and invasion, as well as genomic evaluation, sex control and sex determination, nutrition, biomarkers for disease, and quantitative trait loci mapping for marker-assisted selection in ...
Yan-He Li, Han-Ping Wang
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Genotyping by sequencing for SNP marker development in onion
Genome, 2020Onion (Allium cepa) is not highly tractable for development of molecular markers due to its large (16 gigabases per 1C) nuclear genome. Single nucleotide polymorphisms (SNPs) are useful for genetic characterization and marker-aided selection of onion because of codominance and common occurrence in elite germplasm. We completed genotyping by sequencing
Labate, Joanne A. +2 more
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Multiplexed ISSR Genotyping by Sequencing (MIG-Seq)
2023Multiplexed inter-simple sequence repeat (ISSR) genotyping by sequencing (MIG-seq) is a simple, rapid, and inexpensive method for detecting single-nucleotide polymorphisms (SNPs) using next-generation sequencing (NGS). The advantages of MIG-seq include easy application to various species without prior genetic information. In addition, this method opens
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Multiplexed genotyping with sequence-tagged molecular inversion probes
Nature Biotechnology, 2003We report on the development of molecular inversion probe (MIP) genotyping, an efficient technology for large-scale single nucleotide polymorphism (SNP) analysis. This technique uses MIPs to produce inverted sequences, which undergo a unimolecular rearrangement and are then amplified by PCR using common primers and analyzed using universal sequence tag
Paul, Hardenbol +10 more
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Genotyping-by-Sequencing and Its Application to Oat Genomic Research
2017Genotyping-by-sequencing (GBS) has emerged as a useful genomic approach for sampling genome-wide genetic variation, performing genome-wide association mapping, and conducting genomic selection. It is a combined one-step process of SNP marker discovery and genotyping through genome reduction with restriction enzymes and SNP calling with or without a ...
Yong-Bi, Fu, Mo-Hua, Yang
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The Restriction Enzyme Target Approach to Genotyping by Sequencing (GBS)
2014The modified genotyping by sequencing method described here emphasizes verifying the success of each library ligation by performing individual PCRs, before preparing the pool of barcoded amplicons to be sequenced. Although this extra step might seem excessive, it will give peace of mind to the researcher knowing each individual is represented in the ...
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