Results 221 to 230 of about 5,436,069 (302)

Compact 9dBEs Enable Efficient and Precise Genome Editing in Mammalian Cells and In Vivo

Advanced Science, EarlyView.
As a compact type II‐D system, the Cas9d‐based platform holds great potential for in vivo applications. Through rational engineering, its derived base editors (9dBEs) enable efficient disease modeling while facilitating single‐vector AAV delivery for in vivo genome editing. These miniature tools offer a robust strategy for basic research and biomedical
Qingquan Xiao, Zhijin Tian, Luqi Weng, Kun Xu, Dingyi Han, Zhaowei Wu, Junnan Li, Shangpu Li, Qijing Zhang, Xiaohui Zhao, Guoling Li, Yinghui Wei, Zhen Gu   +12 more
wiley   +1 more source

Development of a PCR-based technique for genotyping UGT1A1 gene and distribution of rs3064744 alleles in the Russian population. [PDF]

Front Genet
Vinokurov MA, Mironov KO, Yurchuk MS, Akimkin VG.   +3 more
europepmc   +1 more source

Enviromics and Abiotic Enviromics: Enhancing Stress Biology and Plant Resilience Breeding

Advanced Science, EarlyView.
Environmental stressors are captured through high‐throughput envirotyping and integrated into an enviromic framework with genomic, transcriptomic, phenomic, and environmental data. AI/ML models enable prediction and guide marker‐assisted selection, genomic selection, genome editing, and gene pyramiding toward improved abiotic stress tolerance, yield ...
Guangchao Sun, Jose Luis Araus, James C. Schnable, Jie Xu, Henry T. Nguyen, Yunbi Xu, Yanli Lu   +6 more
wiley   +1 more source

A next-generation sequencing approach for high-resolution S-locus genotyping in apricot. [PDF]

Sci Rep
Lora J, Torres A, Hormaza JI, Rodrigo J, Hedhly A.   +4 more
europepmc   +1 more source

The Mitogen‐Activated Protein Kinase GhNTF3 Modulates the Arbuscular Mycorrhizal Symbiosis‐Immunity Trade‐Off in Cotton by Regulating Salicylic Acid Biosynthesis

Advanced Science, EarlyView.
AM symbiosis induces GhWAK13 to promote fungal colonization. GhNTF3 negatively regulates symbiosis by promoting SA biosynthesis. GhWAK13 sequesters GhNTF3 at the membrane, limiting its nuclear translocation and relieving SA‐mediated suppression. Under Verticillium stress, GhNTF3 enters the nucleus and interacts with GhJAZ6 to activate SA signaling ...
Shuangjie Jia, Jingshang Wen, Jihang Li, Meina Feng, Yiming He, Ruojun Liu, Qian Liu, Junli Zhang, Kai Cheng, Xiangyu Zhang, Xiao Zhang   +10 more
wiley   +1 more source

Bridging the gap between legacy polymerase chain reaction-based microsatellite data with high-throughput sequencing data for conservation genomics. [PDF]

J Hered
Salih D, Armstrong EE, Robbins CT, Waits LP, Kelley JL.   +4 more
europepmc   +1 more source

NICE: A Two‐Step Non‐Invasive Framework for Embryo cfDNA Read Enrichment and Quality Assessment

Advanced Science, EarlyView.
The non‐invasive NICE framework, built on an ensemble stacking machine learning model, prioritizes embryos by analyzing cell‐free DNA from spent culture medium. By integrating multimodal signals, including genomic and epigenetic profiles, this automated approach standardizes morphological assessment without human bias, paving the way for more precise ...
Xueya Zhou, Shu Ding, Zhenyi Zhang, Qiaoling Shangguan, Jie Qiao, Peijie Zhou, Yidong Chen   +6 more
wiley   +1 more source

Multicentre, controlled, randomised and single-blind, adaptive phase IV protocol to evaluate effectiveness and cost-effectiveness of a pre-emptive genotyping strategy to optimise tacrolimus dosage in a pretransplant chronic kidney disease population cohort: iPHARMGx TRANSPGx nested clinical trial. [PDF]

BMJ Open
Seco-Meseguer E, Stewart S, Diago-Sempere E, Jiménez C, Macías Carmona N, Fernández Solís J, Vila Santandreu A, Valero San Cecilio R, López Jiménez V, Carmona-Rodríguez M, López-Fernández LA, Imaz-Iglesia I, García-Saiz MDM, Farré M, Rodriguez-Jimenez C, Sanabria-Cabrera J, Rosas-Alonso R, García-García I, Borobia AM, iPHARMGx study group.   +19 more
europepmc   +1 more source

An Effector TaCRVP From Tuta Absoluta Oral Secretion Impairs H2O2‐Mediated Plant Defense by Targeting Tomato SlCAT2

Advanced Science, EarlyView.
The Tuta absoluta salivary effector TaCRVP subverts tomato immunity by hijacking host catalase SlCAT2. Through a sophisticated dual mechanism, TaCRVP simultaneously promotes SlCAT2 tetramerization for activation and blocks its SlAdBiL‐mediated degradation for stabilization.
Jianquan Yan, Youdan Zhang, Zhujun Wang, Huatao Tang, Huiping Liu, Zi Chen, Shuai Liu, Jiayi Shi, Lu Peng, Youming Hou   +9 more
wiley   +1 more source

Genotype distribution of high-risk and potential high-risk HPV types among women in different screening and clinic settings in Ghana and Kenya. [PDF]

PLoS One
Kwesi-Maliepaard EM, Osea-Larbi JA, Nyamari CK, Alhassan Y, Sromani AK, Kotey VM, Darko S, Tackie R, Kiome R, Mohammed AM, Buadii E, Quaye EK, Akligoh H, Ibrahim BA, Kaba P, Amoako SF, Kotey E, Agyemang S, Ngoi JM, Paemka L, Darkwa K, Degley T, Siebu NE, Olwal CO, Morang'a CM, Manu A, Hutchful D, Amoako E, Onyambu FG, Bediako Y.   +29 more
europepmc   +1 more source