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Molecular Techniques for Genotyping
2020Genotyping is a process of determining the genetic constituent/genetic makeup “genotype” of an organism by examining the individual DNA sequence and comparing to a reference or other individual sequence. It helps the researchers to explore the genetic constitution, genetic linkages or variations like Single Nucleotide Polymorphisms (SNP) or multi ...
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Biochemical techniques for genotype characterization
1990The techniques mainly used for genotype identification and characterization are presented and discussed in respect to their suitability and performance. Procedures are described for distinguishing between varieties and wild types of oats (Avena spp.) as well as varieties and provenances of Jerusalem artichoke (Helianthus tuberosus L.) using ...
G. F. Rühl, K.-H. Standke, K. Marmulla
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Journal of Clinical Virology, 2011
There is the need for a rapid, economical method for genotyping Hepatitis B virus (HBV) to support clinical practice.To develop a novel HBV genotyping process using genotype specific loop mediated isothermal amplification (LAMP).HBV genotypes B and C specific LAMP methods were evaluated using standard panel.
Zhejun, Cai +4 more
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There is the need for a rapid, economical method for genotyping Hepatitis B virus (HBV) to support clinical practice.To develop a novel HBV genotyping process using genotype specific loop mediated isothermal amplification (LAMP).HBV genotypes B and C specific LAMP methods were evaluated using standard panel.
Zhejun, Cai +4 more
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Molecular Biology Techniques for the Identification and Genotyping of Microorganisms
2019The advent in molecular biology techniques has enabled, to a great extent, numerous identification and detection techniques of microorganisms by amplifying specific conserved DNA sequences. From time-to-time, the tremendous modifications have been employed in search of a rapid and inexpensive microbial identification method and now a whole genome to be
Nisarg Gohil +3 more
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Structuring Genotype X Environment Interaction by Regression Techniques
2021The investigation of the structure of genotype-by-environment interaction is an important topic in multi-environment trials, in which a series of tests are undertaken across multiple environmental conditions. This study proposes a generalisation of joint regression analysis for situations when the response (e.g. yield) is non-linear across environments
Dulce Gamito Santinhos Pereira +4 more
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[A simple technique for the genotyping of TH01 locus].
Nihon hoigaku zasshi = The Japanese journal of legal medicine, 1998It is difficult to differentiate between type 9.3 and 10 at the TH01 locus. Therefore, we designed three primers including allele-specific primer for the type 9.3. Our primer sets gave shorter fragments ranging from 62 to 81 bp than those in other reports, allowing each allelic band to be resolved by 10% non-denaturing polyacrylamide gel ...
K, Umetsu +3 more
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Genotyping with a dried blood spot method: A useful technique for application in pharmacogenetics
Clinica Chimica Acta, 2008Several commercial DNA isolation kits are available for extracting the genomic DNA from the ethylene diamine tetra-acetic acid (EDTA) whole blood samples. To obtain DNA from whole blood these DNA isolation procedures require quite some hands on time and are rather expensive.
Wijnen, P.A.H.M. +9 more
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PCR-CTPP: a new genotyping technique in the era of genetic epidemiology
Expert Review of Molecular Diagnostics, 2001A new PCR method, PCR-CTPP (polymerase chain reaction with confronting two-pair primers) was invented to genotype a relatively large number of samples in a cost-effective and time-saving manner. In this method, allele-specific DNA products are amplified by means of applying appropriately designed two-pair primers (four primers) into an ordinary PCR ...
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Pyrosequencing genotype storage techniques.
Methods in molecular biology (Clifton, N.J.), 2007Data storage and data coordination are important aspects of project design and execution. Pyrosequencing technology allows thousands of data-points to be collected per day. Consequently, a consistent and reliable method of data input and storage is vital. This chapter discusses the strengths and weaknesses of data storage systems.
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Optimization and Application of Genotyping and Identification Techniques for Pneumocystis jirovecii
2022Pneumocystis species are obligate fungi capable of causing opportunistic infection called Pneumocystis Pneumonia (PCP) in those who are immunocompromised, often resulting in serious disease and a high mortality rate. Due to host specificity and inability to conduct in vitro studies, molecular methods have stepped in to undertake the bulk of ...
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