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Molecular Techniques for Genotyping

2020
Genotyping is a process of determining the genetic constituent/genetic makeup “genotype” of an organism by examining the individual DNA sequence and comparing to a reference or other individual sequence. It helps the researchers to explore the genetic constitution, genetic linkages or variations like Single Nucleotide Polymorphisms (SNP) or multi ...
openaire   +1 more source

Evaluation of fecal DNA preservation techniques and effects of sample age and diet on genotyping success

Journal of Wildlife Management, 2011
M. Panasci   +6 more
exaly   +2 more sources

Genotyping-by-sequencing performance in selected livestock species.

Genomics, 2018
Application of next generation sequencing for large scale genotyping in livestock is limited by high costs and challenging data analysis process. However, available restriction enzyme-based enrichment techniques like e.g.
A. Gurgul   +7 more
semanticscholar   +1 more source

Development of a novel genotype-specific loop-mediated isothermal amplification technique for Hepatitis B virus genotypes B and C genotyping and quantification

Journal of Clinical Virology, 2011
There is the need for a rapid, economical method for genotyping Hepatitis B virus (HBV) to support clinical practice.To develop a novel HBV genotyping process using genotype specific loop mediated isothermal amplification (LAMP).HBV genotypes B and C specific LAMP methods were evaluated using standard panel.
Zhejun, Cai   +4 more
openaire   +2 more sources

Liquid biopsy of fine-needle aspiration supernatant for lung cancer genotyping.

Lung Cancer, 2018
BACKGROUND Tumor genotyping is transforming lung cancer care but requires adequate tumor tissue. Advances in minimally invasive biopsy techniques have increased access to difficult-to-access lesions, but often result in smaller samples.
N. Guibert   +10 more
semanticscholar   +1 more source

[A simple technique for the genotyping of TH01 locus].

Nihon hoigaku zasshi = The Japanese journal of legal medicine, 1998
It is difficult to differentiate between type 9.3 and 10 at the TH01 locus. Therefore, we designed three primers including allele-specific primer for the type 9.3. Our primer sets gave shorter fragments ranging from 62 to 81 bp than those in other reports, allowing each allelic band to be resolved by 10% non-denaturing polyacrylamide gel ...
K, Umetsu   +3 more
openaire   +1 more source

PCR-CTPP: a new genotyping technique in the era of genetic epidemiology

Expert Review of Molecular Diagnostics, 2001
A new PCR method, PCR-CTPP (polymerase chain reaction with confronting two-pair primers) was invented to genotype a relatively large number of samples in a cost-effective and time-saving manner. In this method, allele-specific DNA products are amplified by means of applying appropriately designed two-pair primers (four primers) into an ordinary PCR ...
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Structuring Genotype X Environment Interaction by Regression Techniques

2021
The investigation of the structure of genotype-by-environment interaction is an important topic in multi-environment trials, in which a series of tests are undertaken across multiple environmental conditions. This study proposes a generalisation of joint regression analysis for situations when the response (e.g. yield) is non-linear across environments
Dulce Gamito Santinhos Pereira   +4 more
openaire   +1 more source

Pyrosequencing genotype storage techniques.

Methods in molecular biology (Clifton, N.J.), 2007
Data storage and data coordination are important aspects of project design and execution. Pyrosequencing technology allows thousands of data-points to be collected per day. Consequently, a consistent and reliable method of data input and storage is vital. This chapter discusses the strengths and weaknesses of data storage systems.
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Optimization and Application of Genotyping and Identification Techniques for Pneumocystis jirovecii

2022
Pneumocystis species are obligate fungi capable of causing opportunistic infection called Pneumocystis Pneumonia (PCP) in those who are immunocompromised, often resulting in serious disease and a high mortality rate. Due to host specificity and inability to conduct in vitro studies, molecular methods have stepped in to undertake the bulk of ...
openaire   +1 more source

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