Results 1 to 10 of about 14,736 (197)

Microscopic Observation of Membrane Fusion between Giant Liposomes and Baculovirus Budded Viruses Activated by the Release of a Caged Proton [PDF]

Membranes, 2023
Baculovirus (Autographa californica multiple nucleopolyhedrovirus, AcMNPV) is an envelope virus possessing a fusogenic protein, GP64, which can be activated under weak acidic conditions close to those in endosomes.
Misako Nishigami, Yuki Uno, Kanta Tsumoto   +2 more
doaj   +2 more sources

Reconstitution and functional studies of hamster P-glycoprotein in giant liposomes. [PDF]

PLoS ONE, 2018
This paper describes the preparation of giant unilamellar vesicles with reconstituted hamster P-glycoprotein (Pgp, ABCB1) for studying the transport activity of this efflux pump in individual liposomes using optical microscopy.
SooHyun Park, Sheereen Majd
doaj   +2 more sources

Postsynthetic Photocontrol of Giant Liposomes via Fusion-Based Photolipid Doping. [PDF]

Langmuir, 2022
We report on photolipid doping of giant unilamellar vesicles (GUVs) via vesicle fusion with small unilamellar photolipid vesicles (pSUVs), which enables retroactive optical control of the membrane properties. We observe that vesicle fusion is light-dependent, if the phospholipids are neutral.
Pritzl SD, Morstein J, Kahler S, Konrad DB, Trauner D, Lohmüller T.   +5 more
europepmc   +6 more sources

Design and Manufacture of a Low-Cost Microfluidic System for the Synthesis of Giant Liposomes for the Encapsulation of Yeast Homologues: Applications in the Screening of Membrane-Active Peptide Libraries [PDF]

Micromachines, 2021
The discovery of new membrane-active peptides (MAPs) is an area of considerable interest in modern biotechnology considering their ample applicability in several fields ranging from the development of novel delivery vehicles (via cell-penetrating ...
Saúl C. Gómez, Valentina Quezada, Isabella Quiroz, Carolina Muñoz-Camargo, Johann F. Osma, Luis H. Reyes, Juan C. Cruz   +6 more
doaj   +2 more sources

Mechanisms of Self-Assembly of Giant Unilamellar Vesicles in the Army Liposome Formulation (ALF) Family of Vaccine Adjuvants [PDF]

Pharmaceutics
Background/Objectives: Army Liposome Formulation with QS21 (ALFQ) is a vaccine adjuvant formulation consisting of liposomes that contain saturated zwitterionic and anionic phospholipids, 55 mol% cholesterol, and small molar amounts of monophosphoryl ...
Calin Nicolescu, Essie Komla, Mangala Rao, Gary R. Matyas, Carl R. Alving   +4 more
doaj   +2 more sources

Facile and versatile PDMS-glass capillary double emulsion formation device coupled with rapid purification toward microfluidic giant liposome generation [PDF]

Microsystems & Nanoengineering
The exceptional ability of liposomes to mimic a cellular lipid membrane makes them invaluable tools in biomembrane studies and bottom-up synthetic biology.
Mostafa Bakouei, Ali Kalantarifard, Indraja Sundara Raju, Tatiana Avsievich, Lauri Rannaste, Marjut Kreivi, Caglar Elbuken   +6 more
doaj   +2 more sources

On-Chip Inverted Emulsion Method for Fast Giant Vesicle Production, Handling, and Analysis

Micromachines, 2020
Liposomes and giant unilamellar vesicles (GUVs) in particular are excellent compartments for constructing artificial cells. Traditionally, their use requires bench-top vesicle growth, followed by experimentation under a microscope.
Naresh Yandrapalli, Tina Seemann, Tom Robinson   +2 more
doaj   +2 more sources

Collapsin Response Mediator Protein 2 (CRMP2) Modulates Induction of the Mitochondrial Permeability Transition Pore in a Knock-In Mouse Model of Alzheimer’s Disease [PDF]

Cells
Hyperphosphorylated collapsin response mediator protein 2 (CRMP2) is elevated in the cerebral cortex of an APP-SAA knock-in mouse model of Alzheimer’s disease and binds the adenine nucleotide translocase (ANT) in a phosphorylation-dependent manner.
Tatiana Brustovetsky, Rajesh Khanna, Nickolay Brustovetsky   +2 more
doaj   +2 more sources

Encapsulation of protein/DNA complexes into unilamellar liposomes via annexin-mediated membrane recruitment and sonication [PDF]

Cell Reports: Methods
Summary: This paper reports an effective protocol to encapsulate native protein/DNA complexes into unilamellar vesicles composed of natural lipids without the use of organic solvents, in physiological buffers, and at low protein/DNA concentrations.
Michael Burger, Finn Brigger, Valeria Mantella, Jean-Christophe Leroux   +3 more
doaj   +2 more sources

Nano-liposomes: Historical Perspectives and Major Advances in Cancer Therapy. A critical Review

British Journal of Pharmacy, 2023
Since the discovery of liposomes by Bangham and co-workers in 1960, giant strides in the field of liposomes as drug carriers for cancer therapy have been achieved. From the very first generation of liposomes known as conventional liposomes to the stealth
Ibilola Mary Cardoso-Daodu , Margaret Okonawan Ilomuanya, Chukwuemeka Paul Azubuike   +2 more
doaj   +1 more source