Results 131 to 140 of about 6,246 (170)

Engineering semi-permeable giant liposomes

Chemical Communications, 2023
Giant unilamellar vesicles (GUVs) with semi-permeable nature are constructed as building blocks for synthetic cells.
Sreelakshmi Radhakrishnan, Karthika S Nair, Samir Nandi, Harsha Bajaj   +3 more
openaire   +2 more sources

Methods for preparation of giant liposomes

Keerti Mishra, Pooja Yadav
exaly   +2 more sources

Efficient liposome fusion to phase-separated giant vesicles

Biophysical Journal, 2023
Lateral phase heterogeneity in biomembranes can govern cellular functions and may serve as a platform for enrichment or depletion of membrane-anchored molecules. In this work, we address the question of how the process of membrane fusion is affected by the membrane phase state (fluid or gel) and by phase coexistence, as well as the effects of fusion ...
Rafaela R.M. Cavalcanti, Rafael B. Lira, Eleanor J. Ewins, Rumiana Dimova, Karin A. Riske   +4 more
openaire   +3 more sources

Preparation of giant liposomes

Biochimica et Biophysica Acta (BBA) - Biomembranes, 1982
A method is described for the preparation of giant unilamellar lipid vesicles that are stable in electrolyte solution. In general, it involves dialysis of lipid and indifferent solute in a water-miscible organic solvent against an aqueous buffer. During dialysis the concentration of organic solvent decreases so that vesicles form under conditions where
N, Oku, J F, Scheerer, R C, MacDonald
openaire   +2 more sources

Controlling nucleation in giant liposomes

Chem. Commun., 2014
Confinement effects in giant liposomes lead to dramatic stabilization of amorphous calcium carbonate (ACC), intermediate stabilization of amorphous strontium carbonate (ASC), but has no effect on the precipitation of barium carbonate.
Chantel C, Tester, Michael L, Whittaker, Derk, Joester   +2 more
openaire   +2 more sources

Interactions of DNA with giant liposomes

Chemistry and Physics of Lipids, 1999
DNA interactions with the bilayers of cationic liposomes were studied using a novel model experiment: DNAs were locally injected by a micropipette to a part of a giant unilamellar vesicle. The resulting phenomena were directly observed in optical microscope.
M I, Angelova, I, Tsoneva
openaire   +2 more sources

A novel technique for preparation of monodisperse giant liposomes

Chemical Communications, 2003
A novel technique for the preparation of monodisperse giant liposomes has been developed based on a combination of micro-patterning of ITO glass slides with lipid solution and electroformation. The average diameter of the produced liposomes is determined by size of the micro-pattern features.
Pietro, Taylor, Chun, Xu, Paul D I, Fletcher, Vesselin N, Paunov   +3 more
openaire   +2 more sources

Giant liposome spreading on a silicon wall

Journal of Applied Physics, 2011
The spreading of a giant liposome on a vertical silicon wall can be realized when the gravity effect on the spreading is negligible. The dynamic spreading process is experimentally observed and recorded, while the evolution of the liposome shape and the change of the contact angle are quantitatively examined.
Ying Zhang, Yan Chen, Hui Fan
openaire   +1 more source

Preparation of connexin43‐integrated giant Liposomes by a baculovirus expression–liposome fusion method

Biotechnology and Bioengineering, 2010
AbstractConnexin‐43 (Cx43) containing giant liposomes (GL) were prepared by a baculovirus expression–liposome fusion method. Recombinant budded viruses expressing Cx43 were prepared and then fused with GLs containing DOPG/DOPC at pH 4.5. Connexon formation on the GL membrane was observed by transmission electron microscope.
Koki, Kamiya, Kanta, Tsumoto, Satoko, Arakawa, Shigeomi, Shimizu, Ikuo, Morita, Tetsuro, Yoshimura, Kazunari, Akiyoshi   +6 more
openaire   +2 more sources

Electroformation of giant liposomes in microfluidic channels

Measurement Science and Technology, 2005
We present an on-chip method to produce various types of giant liposomes using electroformation in microfluidic channels. These channels were sandwiched between glass slides coated with indium tin oxide (ITO) electrodes. Giant liposomes were formed inside the channels by applying an ac voltage.
Kuribayashi, K., Tresset, G., Coquet, P., Fujita, H., Takeuchi, S.   +4 more
openaire   +1 more source