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Electroformation and Collection of Giant Liposomes on a Microchip
ECS Meeting Abstracts, 2018Due to its micrometer scale and similar membrane structure as the cell membrane, the giant liposome becomes an important lipid structure and is widely used in many research fields as a cell or cell membrane model, microreactor, carrier, and so on. Electroformation is an important preparing method for giant liposomes.
Zhenyu Wang +6 more
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Dynamic Behavior of Giant Liposomes at Desired Osmotic Pressures
Langmuir, 2009To apply accurate and uniform osmotic pressures to liposomes, they can be formed using the spontaneous transfer method in solutions with different osmolarities. The majority of liposomes unexpectedly opened large holes (several micrometers in diameter) in response to the osmotic pressure regardless of its strength, that is, the difference between the ...
Masae, Ohno +3 more
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Preparation and some properties of giant liposomes and proteoliposomes.
Journal of biochemistry, 1987Optimal conditions for formation of giant liposomes and proteoliposomes were investigated. A suspension of small unilamellar vesicles made of various phospholipids in a buffer of 0-3 M KCl, 0.1 mM EDTA, and 20 mM MOPS (pH 7.0) was subjected to a freeze-thaw treatment. Giant multilamellar liposomes of diameter ranging from 10 to 60 microns were found to
K, Higashi +3 more
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Spreading of giant liposomes on anisotropically patterned substrates
Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2013The dynamic spreading of giant liposomes on anisotropically patterned substrates is investigated experimentally on vertically fixed spreading substrates. The giant liposomes are prepared through electroformation method. The substrates with anisotropic grooves on the surfaces are fabricated on silicon wafers by photolithography and dry etching.
Ying Zhang, Yan Chen, Hui Fan
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Adhesion of Giant Liposomes as Observed by Light Microscopy
Langmuir, 1997Giant liposomes composed of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and cholesterol are imparted with cationic or anionic charge by incorporation of 2−20% cationic or anionic lipids. Although two giant liposomes of like charge do not adhere, two giant liposomes of opposite charge will (depending upon the % of ionic lipid) either adhere ...
F. M. Menger, J. S. Keiper, S. J. Lee
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Reconstruction of motile actin networks in giant liposome
2010 International Symposium on Micro-NanoMechatronics and Human Science, 2010To construct a simple model cellular system exhibiting the property of self-propelled motion, cell-sized giant liposomes encapsulating desired amounts of actoHMM, a mixture of actin filament (F-actin) and heavy meromyosin (HMM, an actin-related molecular motor), have been prepared.
Kingo Takiguchi +4 more
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Coating of POPC Giant Liposomes with Hydrophobized Polysaccharide
Chemistry Letters, 1998Abstract POPC giant liposomes were incubated with pullulan that was hydrophobized by cholesteryl groups and labelled by a fluorescent marker (FITC). Confocal laser microscopy clearly showed coating of the outer surface of the liposomes with the polymer.
Takehiko Ueda +4 more
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Population Analysis of Structural Properties of Giant Liposomes by Flow Cytometry
Langmuir, 2009We used fluorescence flow cytometry to analyze the structural properties of populations of giant liposomes formed by different preparation methods. The inner aqueous volumes and nominal membrane surface areas of a large number of individual liposomes were measured simultaneously by using fluorescent markers.
Kazuya, Nishimura +8 more
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Giant Liposome Microreactors for Controlled Production of Calcium Phosphate Crystals
Langmuir, 2004Calcium phosphates are among the most important biominerals in living organisms, where they play both a mechanical and a calcium storage role. Their growth in vivo is under strong biological control, and this process occurs in closed spaces. Our aim in this paper is to describe a microreactor system able to control the mineralization process within ...
Marc, Michel +6 more
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Morphological and topological transformations that are induced into cell-sized giant liposomes
2011 International Symposium on Micro-NanoMechatronics and Human Science, 2011Morphological and topological changes of biological membranes play essential roles in cellular activities, such as endocytosis and exocytosis. Here, real-time imaging of giant liposomes using optical dark-field microscopy reveals that the lipid bilayer membrane possesses the ability to undergo topological transformation through interactions with ...
Kingo Takiguchi +2 more
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