Results 181 to 190 of about 431,797 (248)

Preparation of giant liposomes

Biochimica et Biophysica Acta (BBA) - Biomembranes, 1982
A method is described for the preparation of giant unilamellar lipid vesicles that are stable in electrolyte solution. In general, it involves dialysis of lipid and indifferent solute in a water-miscible organic solvent against an aqueous buffer. During dialysis the concentration of organic solvent decreases so that vesicles form under conditions where
N, Oku, J F, Scheerer, R C, MacDonald
openaire   +2 more sources

Interactions of DNA with giant liposomes

Chemistry and Physics of Lipids, 1999
DNA interactions with the bilayers of cationic liposomes were studied using a novel model experiment: DNAs were locally injected by a micropipette to a part of a giant unilamellar vesicle. The resulting phenomena were directly observed in optical microscope.
M I, Angelova, I, Tsoneva
openaire   +2 more sources

Effects of Sugars on Giant Unilamellar Vesicle Preparation, Fusion, PCR in Liposomes, and Pore Formation.

Langmuir, 2022
The water-in-oil emulsion transfer method was developed for preparing giant unilamellar vesicles (GUVs) and is useful for studying cellular functions under conditions that mimic cellular environments.
Kyoka Kajii, Ayu Shimomura, Mika T Higashide, Masaya Oki, Gakushi Tsuji   +4 more
semanticscholar   +1 more source

Preparation of connexin43‐integrated giant Liposomes by a baculovirus expression–liposome fusion method

Biotechnology and Bioengineering, 2010
AbstractConnexin‐43 (Cx43) containing giant liposomes (GL) were prepared by a baculovirus expression–liposome fusion method. Recombinant budded viruses expressing Cx43 were prepared and then fused with GLs containing DOPG/DOPC at pH 4.5. Connexon formation on the GL membrane was observed by transmission electron microscope.
Koki, Kamiya, Kanta, Tsumoto, Satoko, Arakawa, Shigeomi, Shimizu, Ikuo, Morita, Tetsuro, Yoshimura, Kazunari, Akiyoshi   +6 more
openaire   +2 more sources

Electroformation of giant liposomes in microfluidic channels

Measurement Science and Technology, 2005
We present an on-chip method to produce various types of giant liposomes using electroformation in microfluidic channels. These channels were sandwiched between glass slides coated with indium tin oxide (ITO) electrodes. Giant liposomes were formed inside the channels by applying an ac voltage.
Kuribayashi, K., Tresset, G., Coquet, P., Fujita, H., Takeuchi, S.   +4 more
openaire   +1 more source

Giant liposome spreading on a silicon wall

Journal of Applied Physics, 2011
The spreading of a giant liposome on a vertical silicon wall can be realized when the gravity effect on the spreading is negligible. The dynamic spreading process is experimentally observed and recorded, while the evolution of the liposome shape and the change of the contact angle are quantitatively examined.
Ying Zhang, Yan Chen, Hui Fan
openaire   +1 more source

Local Deformation in Phase-Separated Giant Liposomes via Photo-Induced Peptide Nanofiber Formation.

Langmuir
Dynamic morphology changes in cellular systems are achieved through spatiotemporal control of cytoskeletal assembly at the plasma membrane, such as pseudopodia formation in amoeba.
Yingbing Liang, Yasunari Takaki, H. Inaba, K. Matsuura   +3 more
semanticscholar   +1 more source

Dynamic Behavior of Giant Liposomes at Desired Osmotic Pressures

Langmuir, 2009
To apply accurate and uniform osmotic pressures to liposomes, they can be formed using the spontaneous transfer method in solutions with different osmolarities. The majority of liposomes unexpectedly opened large holes (several micrometers in diameter) in response to the osmotic pressure regardless of its strength, that is, the difference between the ...
Masae, Ohno, Tsutomu, Hamada, Kingo, Takiguchi, Michio, Homma   +3 more
openaire   +2 more sources

A novel technique for preparation of monodisperse giant liposomes

Chemical Communications, 2003
A novel technique for the preparation of monodisperse giant liposomes has been developed based on a combination of micro-patterning of ITO glass slides with lipid solution and electroformation. The average diameter of the produced liposomes is determined by size of the micro-pattern features.
Pietro, Taylor, Chun, Xu, Paul D I, Fletcher, Vesselin N, Paunov   +3 more
openaire   +2 more sources

Spontaneous Formation of Giant Liposomes from Neutral Phospholipids

Chemistry Letters, 1997
Abstract Giant liposomes were formed spontaneously from neutral phospholipids in an aqueous solution containing MgCl2. Based on both dark-field microscopy and transmission electron microscopy, we concluded that these giant liposomes are unilamellar.
Nobuyuki Magome, Tatsunobu Takemura, Kenichi Yoshikawa   +2 more
openaire   +1 more source