Results 11 to 20 of about 431,797 (248)

Electroformation of giant unilamellar vesicles from large liposomes [PDF]

The European Physical Journal Special Topics
AbstractGiant unilamellar vesicles (GUVs) are widely used as model systems for biological membranes to study membrane-related processes in a precisely controlled in vitro environment, owing to their biophysical properties. The classical technique for the formation of giant liposomes starts with the dissolution of lipids or lipid mixtures in an organic ...
Huriye D. Uzun, Zeynep Tiris, Maiko Czarnetzki, Rosa L. López-Marqués, Thomas Günther Pomorski   +4 more
openaire   +4 more sources

Shadow electrochemiluminescence imaging of giant liposomes opening at polarized electrodes

The Analyst
In this work, the release of giant liposome (∼100 μm in diameter) content was imaged by shadow electrochemiluminescence (ECL) microscopy.
Ben Trad, Fatma, Delacotte, Jérôme, Lemaître, Frédéric, Guille-Collignon, Manon, Arbault, Stéphane, Sojic, Neso, Labbé, Eric, Buriez, Olivier   +7 more
openaire   +4 more sources

Reconstitution of Multidrug Resistance Efflux Pumps in Giant Liposomes [PDF]

Biophysical Journal, 2015
Multidrug resistance (MDR), a phenomenon in which cells become resistant to a number of drugs, is a growing obstacle for treatment of cancer and bacterial infections. A group of membrane transporters, known as MDR efflux pumps that bind to a wide range of foreign molecules and pump them out of the cell, are suspected to be the main contributors to MDR.
Park, SooHyun, Kang, You Jung, Majd, Sheereen   +2 more
openaire   +2 more sources

Diffusion of Single-Pass Transmembrane Receptors: From the Plasma Membrane into Giant Liposomes. [PDF]

J Membr Biol, 2017
To quantitatively examine the effect of membrane organization on lateral diffusion, we studied fluorescent carbocyanine lipid analogues and EGFP-tagged, single-pass transmembrane proteins in systems of decreasing complexity: (i) the plasma membrane (PM ...
Worch R, Petrášek Z, Schwille P, Weidemann T.   +3 more
europepmc   +2 more sources

Application of Various Types of Liposomes in Drug Delivery Systems [PDF]

Advanced Pharmaceutical Bulletin, 2017
Liposomes, due to their various forms, require further exploration. These structures can deliver both hydrophilic and hydrophobic drugs for cancer, antibacterial, antifungal, immunomodulation, diagnostics, ophtalmica, vaccines, enzymes and genetic ...
Mehran Alavi, Naser Karimi, Mohsen Safaei   +2 more
doaj   +1 more source

A method for an efficient formation of giant vesicles of neutral phospholipids in microarrays using a microplotter equipment

Materials Research Express, 2021
A methodology which provides a high efficiency of giant vesicle formation was established using the gentle hydration method and a microplotter equipment.
Juan R Avendaño-Gomez, Obed A Solis-Gonzalez, Haydee González-Martínez, Miguel A Vega-Cuellar   +3 more
doaj   +1 more source

Caspase-8 binding to cardiolipin in giant unilamellar vesicles provides a functional docking platform for bid [PDF]

, 2013
Caspase-8 is involved in death receptor-mediated apoptosis in type II cells, the proapoptotic programme of which is triggered by truncated Bid. Indeed, caspase-8 and Bid are the known intermediates of this signalling pathway.
AH Stegh, AH Stegh, AJ Garcia-Saez, AJ Garcia-Saez, AM Chinnaiyan, Ana-Jesus García-Sáez, B Apellaniz, B Leber, Beate Klösgen, Boris Zhivotovsky, C Scaffidi, D Ardail, D Ardail, D Chandra, D Marsh, D Marsh, D Needham, DW Chang, E Evans, E Krieghoff, E van den Brink-van der Laan, EE Varfolomeev, Eyal Gottlieb, F Gonzalvez, F Gonzalvez, F Gonzalvez, F Gonzalvez, FC Kischkel, François Gonzalvez, G Kroemer, GC Shearman, H Zhang, HR Stennicke, J Zha, Jesus Ayala-Sanmartin, JF Lovell, JH Li, JP Medema, K Milovic-Holm, KH Lee, KM Boatright, L Mathivet, Liberty François-Moutal, M Lamblet, M Lutter, M Lutter, M Muzio, M Muzio, MA Hughes, Mark Perry, MI Angelova, MO Hengartner, MP Boldin, N Keller, Olivier Jalmar, P Juo, P Ratanabanangkoon, Patrice X. Petit, Petra Schwille, PJ Jost, RF Epand, S Zhang, T Granjon, T Kuwana, T Parasassi, Thierry Granjon, VA Torres, VA Torres, W Helfrich, W Rawicz, WC Yeh, X Luo, Y Zaltsman, ZT Schug   +73 more
core   +10 more sources

Detergent-free reconstitution of transmembrane proteins in giant liposomes of complex curvature by the Synthetic Membrane Transfer

bioRxiv
Meena H, Zdanowicz R, Bock-Bierbaum T, Stephan T, Ottsen Knudsen SD, Jakobs S, Daumke O, Pedersen PA, De Franceschi N.   +8 more
europepmc   +2 more sources

Quantification of nanoscale forces in lectin-mediated bacterial attachment and uptake into giant liposomes.

Nanoscale, 2021
Interactions of the bacterial lectin LecA with the host cells glycosphingolipid Gb3 have been shown to be crucial for the cellular uptake of the bacterium Pseudomonas aeruginosa.
Ramin Omidvar, Y. Ayala, Annette Brandel, Lukas Hasenclever, M. Helmstädter, A. Rohrbach, W. Römer, J. Madl   +7 more
semanticscholar   +1 more source

Nanoporous silica-based protocells at multiple scales for designs of life and nanomedicine. [PDF]

, 2015
Various protocell models have been constructed de novo with the bottom-up approach. Here we describe a silica-based protocell composed of a nanoporous amorphous silica core encapsulated within a lipid bilayer built by self-assembly that provides for ...
Brinker, C Jeffrey, Jakobsson, Eric, Sun, Jie, Wang, Yingxiao   +3 more
core   +5 more sources