Results 131 to 140 of about 5,077 (168)

Plasma membrane transbilayer asymmetry of PI(4,5)P₂ drives unconventional secretion of Fibroblast Growth Factor 2. [PDF]

Nat Commun
Kaur M, Lolicato F, Nickel W.
europepmc   +1 more source

Bulk Self-Assembly of Giant, Unilamellar Vesicles

ACS Nano, 2020
The desire to create cell-like models for fundamental science and applications has spurred extensive effort towards creating giant unilamellar vesicles (GUVs). However, a route to selectively self-assemble GUVs in bulk has remained elusive. In bulk solution, membrane-forming molecules such as phospholipids, single-tailed surfactants, and block ...
Anna Wang, James Kindt, Jack W Szostak
exaly   +5 more sources

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Advances in giant unilamellar vesicle preparation techniques and applications

Advances in Colloid and Interface Science, 2023
Giant unilamellar vesicles (GUVs) are versatile and promising cell-sized bio-membrane mimetic platforms. Their applications range from understanding and quantifying membrane biophysical processes to acting as elementary blocks in the bottom-up assembly of synthetic cells.
Harsha Bajaj
exaly   +3 more sources

Protein Reconstitution Inside Giant Unilamellar Vesicles

Annual Review of Biophysics, 2021
Giant unilamellar vesicles (GUVs) have gained great popularity as mimicries for cellular membranes. As their sizes are comfortably above the optical resolution limit, and their lipid composition is easily controlled, they are ideal for quantitative light microscopic investigation of dynamic processes in and on membranes.
Litschel, T., Schwille, P.
openaire   +4 more sources

Starch aided synthesis of giant unilamellar vesicles

Chemistry and Physics of Lipids, 2020
Synthesis of giant unilamellar vesicles (GUVs) of charged and uncharged lipids at physiological salt concentration is presented using the starch hydrogel method as an example of the gel assisted synthesis method. The swelling of the gel is assisted by the presence of a high amount of amylopectin in starch and yields giant-sized vesicles, which are ...
Mohammad, Maoyafikuddin, Meenakshi, Pundir, Rochish, Thaokar   +2 more
openaire   +2 more sources

Microdomain evolution on giant unilamellar vesicles

Biomechanics and Modeling in Mechanobiology, 2012
A chemo-mechanical model is used to capture the formation and evolution of microdomains on the deforming surface of giant unilamellar vesicles. The model is intended for the regime of vesicle dynamics characterized by a distinct difference in time scales between shape change and species transport.
Anand, Embar, John, Dolbow, Eliot, Fried
openaire   +2 more sources

Phase Transition Induced Adhesion of Giant Unilamellar Vesicles

ChemPhysChem, 2009
AbstractCell and vesicle adhesion is believed to be dictated by the balance between a local interaction potential, which represents the sum of all attractive and repulsive forces and the elastic energy. Changing the mechanical properties of the membrane therefore offers a sensitive tool to control vesicle adhesion.
Thomas Franke, Achim Wixforth
exaly   +4 more sources

Refined contour analysis of giant unilamellar vesicles

The European Physical Journal E, 2004
The fluctuation spectrum of giant unilamellar vesicles is measured using a high-resolution contour detection technique. An analysis at higher q vectors than previously achievable is now possible due to technical improvements of the experimental setup and of the detection algorithm.
Pécréaux, J., Döbereiner, H.-G., Prost, J., Joanny, J.-F., Bassereau, P.   +4 more
openaire   +3 more sources

Electrofusion of giant unilamellar vesicles to cells

2015
We describe a method for electroporation-induced fusion of giant unilamellar vesicles (GUVs) with the plasma membrane of adherent cells. Using this method, the area of the cell membrane can be abruptly increased and various lipids can be introduced into the membrane.
Dikla, Raz-Ben Aroush, Shlomit, Yehudai-Resheff, Kinneret, Keren   +2 more
openaire   +2 more sources

Electroformation of giant unilamellar vesicles in saline solution

Colloids and Surfaces B: Biointerfaces, 2016
Giant unilamellar vesicle (GUV) formation on indium tin oxide (ITO) electrodes in saline solution and from charged lipids has proven to be difficult in the past. Yet the best cell membrane models contain charged lipids and require physiological conditions. We present a way to overcome this problem by using plasma cleaned ITO electrodes.
Qingchuan, Li, Xuejing, Wang, Shenghua, Ma, Ying, Zhang, Xiaojun, Han   +4 more
openaire   +2 more sources