Results 141 to 150 of about 5,077 (168)
Some of the next articles are maybe not open access.
Giant unilamellar vesicles with cytoskeleton
2019In this chapter we will give first an overview about the many functions of the cytoskeleton, its composition and structure in various organisms. We will describe next methods to anchor cytoskeletal filaments to membranes of supported lipid bilayers (SLB) and giant unilamellar vesicles (GUV) by several strategies based on charge, amphipathic helices ...
Härtel, T., Schwille, P.
openaire +3 more sources
Domain Sorting in Giant Unilamellar Vesicles Adsorbed on Glass
Langmuir, 2021Giant unilamellar vesicles (GUVs) adsorb to a solid surface and rupture to form a planar bilayer patch. These bilayer patches are used to investigate the properties and functions of biological membranes. Therefore, it is crucial to understand the mechanisms of GUV adsorption. In this study, we investigate the adsorption of phase-separated GUVs on glass
Chiho Kataoka-Hamai, Kohsaku Kawakami
openaire +2 more sources
Spatial effects of photosensitization on morphology of giant unilamellar vesicles
Biophysical Chemistry, 2021Singlet oxygen (1O2) formed through photosensitization may initiate oxidative destruction of biomembranes, however, the influence from the spatial organization of photosensitizers (PS) relative to membranes remains unclear. To clarify this issue, we loaded riboflavin 5'-(dihydrogen phosphate) monosodium (FMN-Na) as a hydrophilic PS into the lumen of ...
Wei, Yuan +7 more
openaire +3 more sources
Laser Tweezer Deformation of Giant Unilamellar Vesicles
2007Two methods are presented for deforming giant unilamellar vesicles with holographic optical tweezers. The first allows ultrahigh spatial- and temporal-resolution optical tracking of membrane deformations, by using embedded silica microspheres in a giant unilamellar vesicle as tracers.
Cory, Poole, Wolfgang, Losert
openaire +2 more sources
Interfacial Phases on Giant Unilamellar Vesicles
ASME 2012 Summer Bioengineering Conference, Parts A and B, 2012Lipid nanodomains in cell membranes are believed to play a significant role in a number of critical cellular processes (Elson, et al., 2010). These include, for example, replication processes in enveloped viruses such as bird flu and HIV and signaling mechanisms underlying pathological conditions such as cancer.
Yanfei Jiang +3 more
openaire +1 more source
Acoustical deformability of giant unilamellar vesicles
The Journal of the Acoustical Society of America, 2017An acoustic standing wave is used to trap and deform giant unilamellar vesicles with a diameter ranging from 10 to 50 μm. The giant unilamellar vesicles are prepared in glucose solution with a bi-layer of DOPC membrane with approximately 10 nm-thickness. They are suspended in a 4 cm2-chamber of an acoustofluidic device.
Liangfei Tian +2 more
openaire +1 more source
Block copolymers in giant unilamellar vesicles with proteins or with phospholipids
Faraday Discussions, 2013Biocompatible, highly water-soluble, nonionic, amphiphilic block copolymers having different hydrophobic blocks and architectures, but similar molecular size and chemical nature of the hydrophilic blocks, were investigated to check for their ability to form hybrid giant unilamellar vesicles with proteins, and for their interactions with giant ...
Regina, Schöps +4 more
openaire +2 more sources
Magnetically Controlled Polymer Giant Unilamellar Vesicles
SmallAbstract Giant unilamellar vesicles (GUVs) are essential tools for mimicking cellular processes such as membrane transport and for applications including sensing or protocell development. While it is often desirable to immobilize GUVs in these contexts, many immobilization approaches are irreversible, or limited ...
Narjes Abdollahi +3 more
openaire +2 more sources
Deformation of giant unilamellar vesicles under osmotic stress
Colloids and Surfaces B: Biointerfaces, 2018Biological membrane plays an important role in maintaining an osmotic equilibrium between the cytoplasm and the extracellular solution of cells. Here, the giant unilamellar vesicles (GUVs) as cell models were used to investigate the effect of osmotic stress on phospholipid membranes. The deformation of GUVs, including inward budding and outward budding,
Wei, Zong +3 more
openaire +2 more sources
Methods for Forming Giant Unilamellar Fatty Acid Vesicles
2021Fatty acids readily assemble into bilayer membranes at a pH near their apparent pKa. Fatty acid vesicles are not only useful for research in the fields of origins of life, soft matter science, biophysics, and drug delivery, but are also cost-effective and easy to manipulate, making them ideal for teaching students about self-assembly and lipid bilayers.
Lauren A, Lowe +2 more
openaire +2 more sources