Results 41 to 50 of about 19,800 (242)

Dual-Channel Fluorescence Assays with Supramolecular Host-Dye Reporter Pairs for Membrane Activity Mapping of Peptides. [PDF]

Angew Chem Int Ed Engl
Membrane‐active peptides (MAPs) such as cell‐penetrating peptides (CPPs) and antimicrobial peptides (AMPs) can translocate across lipid membranes and create membrane pores. The combination of a dye efflux and a supramolecular tandem membrane assay in a dual‐channel format enables an advanced mechanistic characterization and an exploration of the ...
Alnajjar MA, Schöpper SN, Pramod M, Reingolz T, Müller L, Neumann J, Nilam M, Nau WM, Hennig A.   +8 more
europepmc   +2 more sources

On-chip inverted emulsion method for fast giant vesicle production, handling, and analysis

, 2020
Liposomes and giant unilamellar vesicles (GUVs) in particular are excellent compartments for constructing artificial cells. Traditionally, their use requires bench-top vesicle growth, followed by experimentation under a microscope.
Robinson, T., Seemann, T., Yandrapalli, N.   +2 more
core   +1 more source

Active Membrane Fluctuations Studied by Micropipet Aspiration [PDF]

, 2001
We present a detailed analysis of the micropipet experiments recently reported in J-B. Manneville et al., Phys. Rev. Lett. 82, 4356--4359 (1999), including a derivation of the expected behaviour of the membrane tension as a function of the areal strain ...
A. N. Radionov, A. Royant, A.G. Petrov, D. Oesterhelt, E. Boroske, E. Evans, E. Evans, E. Pebay-Peyroula, F. Dumas, F. Faucon, F. Maingret, F. Maingret, G. Niggemann, H. J. Sass, H. Luecke, H. Luecke, J-B. Manneville, J. Heberle, J. Heberle, J. K. Lanyi, J. Prost, J. Prost, J. Prost, J.-B. Manneville, J.-L. Rigaud, M. Bloom, M. Bloom, M. I. Angelova, M. Jansen, M. Kummrow, M. M. Speretto, M. Seigneuret, M. Seigneuret, N. A. Dencher, O. G. Mouritsen, P. Bassereau, P. Méléard, Ph. Méléard, R. Henderson, R. Kwok, R. Lawaczeck, R. Lipowsky, S. Ramaswamy, S. Ramaswamy, S. Ramaswamy, S. Subramaniam, S. Subramaniam, T. Kouyama, U. Haupts, W. Helfrich, W. Kuhlbrandt, Y. Cao, Y. Kimura   +52 more
core   +2 more sources

Membrane Remodeling by Arc/Arg3.1

Frontiers in Molecular Biosciences, 2021
The activity-regulated cytoskeletal-associated protein (Arc, also known as Arg3.1) is an immediate early gene product induced by activity/experience and required for multiple modes of synaptic plasticity.
Per Niklas Hedde, Per Niklas Hedde, Leonel Malacrida, Leonel Malacrida, Barbara Barylko, Derk D. Binns, Joseph P. Albanesi, David M. Jameson   +7 more
doaj   +1 more source

Surfactant-free production of biomimetic giant unilamellar vesicles using PDMS-based microfluidics

Communications Chemistry, 2021
Microfluidic production of giant lipid vesicles presents a paradigm-shift in the development of artificial cells. While production is high-throughput and the lipid vesicles are mono-disperse compared to bulk methods, current technologies rely heavily on ...
N. Yandrapalli, Julien Petit, O. Bäumchen, T. Robinson   +3 more
semanticscholar   +1 more source

Membrane Disintegration Caused by the Steroid Saponin Digitonin Is Related to the Presence of Cholesterol

Molecules, 2015
In the present investigation we studied the molecular mechanisms of the monodesmosidic saponin digitonin on natural and artificial membranes. We measured the hemolytic activity of digitonin on red blood cells (RBCs). Also different lipid membrane models (
Ikhwan Resmala Sudji, Yamunadevi Subburaj, Nataliya Frenkel, Ana J. García-Sáez, Michael Wink   +4 more
doaj   +1 more source

Study on the Application of Electric Field to Giant Vesicles Comprised of 1,2-Dilauroyl-Sn-Glycero-3-Phosphatidylcholine Using Optical Fluorescence Microscopy [PDF]

Materials Research, 2017
The influence of alternating electric field (AC) in the structure and dynamics of giant unilamellar vesicles (GUVs) comprised of 1,2-dilauroyl-sn-glycero-3-phosphatidylcholine (DLPC) is reported.
Yasmine Miguel Serafini Micheletto, Nádya Pesce da Silveira, Alexandre Gonçalves Dal-Bó, Fernando Carlos Giacomelli, Newton Luiz Dias Filho, Tiago Elias Allievi Frizon, Carlos Manuel Marques, André Pierre Schroder   +7 more
doaj   +1 more source

Heat-induced formation of single giant unilamellar vesicles [PDF]

, 2011
Giant unilamellar vesicles (GUVs) are an excellent model system for the investigation of lipid membranes, the study of membrane proteins and ion channels in a biomimetic environment, and in the creation of artificial cells.
Billerit, Celine, Dommersnes, Paul, Jeffries, Gavin, Jesorka, Aldo, Orwar, Owe, Wegrzyn, Ilona   +5 more
core   +1 more source

Rapid preparation of giant unilamellar vesicles. [PDF]

Proceedings of the National Academy of Sciences, 1996
We report here a rapid evaporation method that produces in high yield giant unilamellar vesicles up to 50 microns in diameter. The vesicles are obtained after only 2 min and can be prepared from different phospholipids, including L-alpha-phosphatidylcholine (lecithin), dipalmitoleoyl L-alpha-phosphatidylcholine, and beta-arachidonoyl gamma-palmitoyl L ...
A, Moscho, O, Orwar, D T, Chiu, B P, Modi, R N, Zare   +4 more
openaire   +2 more sources

Using small molecules to facilitate exchange of bicarbonate and chloride anions across liposomal membranes [PDF]

, 2009
Bicarbonate is involved in a wide range of biological processes, which include respiration, regulation of intracellular pH and fertilization. In this study we use a combination of NMR spectroscopy and ion-selective electrode techniques to show that the ...
A Fürstner, AP Davis, AV Koulov, C Yamamoto, D Stefayne, E Gout, ED Steinle, F Hofmeister, FA Norris, FG Riddell, HG Hempling, HM Florman, J Fujinaga, Jeffery T. Davis, JL Sessler, Jose Carlos Iglesias-Sánchez, JR Casey, JW Bennett, JY Choi, K Chang, K Kavallieratos, K Tanigaki, L You, M Nguyen, MS Melvin, NM Koropatkin, NN Gerber, NR Williamson, OA Okunola, Oluyomi A. Okunola, Philip A. Gale, Pilar Prados, PV Santacroce, RA Gottlieb, Roberto Quesada, S Ohkuma, T Sato, Tomás Torroba, V Rizzo, WN Lipscomb, WW Cleland, X Li   +41 more
core   +1 more source