Results 71 to 80 of about 23,127 (230)
In Vitro Membrane Remodeling by ESCRT is Regulated by Negative Feedback from Membrane Tension
iScience, 2019 Summary: Artificial cells can shed new light on the molecular basis for life and hold potential for new chemical technologies. Inspired by how nature dynamically regulates its membrane compartments, we aim to repurpose the endosomal sorting complex ...
Andrew Booth +3 more
doaj +1 more source
Electroformation of Giant Unilamellar Vesicles on Stainless Steel Electrodes [PDF]
ACS Omega, 2017 Giant unilamellar vesicles (GUVs) are well-established model systems for studying membrane structure and dynamics. Electroformation, also referred to as electroswelling, is one of the most prevalent methods for producing GUVs, as it enables modulation of the lipid hydration process to form relatively monodisperse, defect-free vesicles.
Valerio Pereno +6 more
openaire +9 more sources
Nanoporous silica-based protocells at multiple scales for designs of life and nanomedicine. [PDF]
, 2015 Various protocell models have been constructed de novo with the bottom-up approach. Here we describe a silica-based protocell composed of a nanoporous amorphous silica core encapsulated within a lipid bilayer built by self-assembly that provides for ...
Brinker, C Jeffrey +3 more
core +5 more sources
Signature of a non-harmonic potential as revealed from a consistent shape and fluctuation analysis of an adherent membrane [PDF]
, 2014 The interaction of fluid membranes with a scaffold, which can be a planar surface or a more complex structure, is intrinsic to a number of systems - from artificial supported bilayers and vesicles to cellular membranes.
Bihr, Timo +6 more
core +6 more sources
Studying Macromolecular Composition in Cell–Cell Interfaces Using 3D Membrane Reconstitution Systems
Advanced Science, EarlyView.A comprehensive understanding of the interactions between proteins, lipids and glycocalyx components at the immune synapse is still lacking. Here, an artificial and a semi‐artificial model contact system were established to reconstitute the cell‐cell contact in 3D. The model systems enable the examination of macromolecule enrichment or depletion at the
Franziska Ragaller +9 more
wiley +1 more source
Reconstitution of proteins on electroformed giant unilamellar vesicles [PDF]
, 2015 In vitro reconstitution of simplified biological systems from molecular parts has proven to be a powerful method for investigating the biochemical and biophysical principles underlying cellular processes. In recent years, there has been a growing interest in reconstitution of protein-membrane interactions to understand the critical role played by ...
Schmid, E., Richmond, D., Fletcher, D.
openaire +3 more sources
Lipid Lateral Organization on Giant Unilamellar Vesicles Containing Lipopolysaccharides [PDF]
Biophysical Journal, 2011 We developed a new (to our knowledge) protocol to generate giant unilamellar vesicles (GUVs) composed of mixtures of single lipopolysaccharide (LPS) species and Escherichia coli polar lipid extracts. Four different LPSs that differed in the size of the polar headgroup (i.e., LPS smooth > LPS-Ra > LPS-Rc > LPS-Rd) were selected to generate GUVs composed
Kubiak, Jakub +3 more
openaire +4 more sources
Generation of Giant Unilamellar Vesicles (GUVs) Using Polyacrylamide Gels [PDF]
BIO-PROTOCOL, 2020 Giant unilamellar vesicles (GUVs) are a widely used model system for a range of applications including membrane biophysics, drug delivery, and the study of actin dynamics. While several protocols have been developed for their generation in recent years, the use of these techniques involving charged lipid types and buffers of physiological ionic ...
Parigoris, E. +2 more
openaire +4 more sources
Pharmaceutics, 2023 Army Liposome Formulation with QS21 (ALFQ), a vaccine adjuvant preparation, comprises liposomes containing saturated phospholipids, with 55 mol% cholesterol relative to the phospholipids, and two adjuvants, monophosphoryl lipid A (MPLA) and QS21 saponin.
Erwin G. Abucayon +3 more
doaj +1 more source
Block Copolymer Giant Unilamellar Vesicles for High-Throughput Screening
CHIMIA, 2022 Bottom-up synthetic cells offer the potential to study cellular processes with reduced complexity. Giant unilamellar vesicles (GUVs) can mimic cells in their morphological characteristics because their architecture is precisely controllable. We propose a block copolymer-based GUV system that can be used for high-throughput screening. Through droplet
Lukas Heuberger, Cornelia Palivan
openaire +5 more sources