Wrapping Pathways of Anisotropic Dumbbell Particles by Giant Unilamellar Vesicles. [PDF]
Nano Lett, 2023 Endocytosis is a key cellular process involved in the uptake of nutrients, pathogens or the diagnosis and therapy of diseases. Most studies have focused on spherical objects, whereas biologically relevant shapes can be highly anisotropic. In this letter, we use an experimental model system based on Giant Unilamellar Vesicles (GUVs) and dumbbell-shaped ...
Azadbakht A +4 more
europepmc +10 more sources
Light-Switchable Membrane Permeability in Giant Unilamellar Vesicles [PDF]
Pharmaceutics, 2022 In this work, giant unilamellar vesicles (GUVs) were synthesized by blending the natural phospholipid 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) with a photoswitchable amphiphile (1) that undergoes photoisomerization upon irradiation with UV-
Paola Albanese +6 more
doaj +6 more sources
Formation of Giant Unilamellar Vesicles Assisted by Fluorinated Nanoparticles [PDF]
Advanced Science, 2023 In the quest to produce artificial cells, one key challenge that remains to be solved is the recreation of a complex cellular membrane. Among the existing models, giant unilamellar vesicles (GUVs) are particularly interesting due to their intrinsic ...
Jorik Waeterschoot +5 more
doaj +5 more sources
Engineering Phosphatidylserine Containing Asymmetric Giant Unilamellar Vesicles [PDF]
MembranesThe plasma membrane lipid distribution is asymmetric, with several anionic lipid species located in its inner leaflet. Among these, phosphatidylserine (PS) plays a crucial role in various important physiological functions.
Jake McDonough +5 more
doaj +4 more sources
Imaging of photoacoustic-mediated permeabilization of giant unilamellar vesicles (GUVs) [PDF]
Scientific Reports, 2021 Target delivery of large foreign materials to cells requires transient permeabilization of the cell membrane without toxicity. Giant unilamellar vesicles (GUVs) mimic the phospholipid bilayer of the cell membrane and are also useful drug delivery ...
Diogo A. Pereira +9 more
doaj +4 more sources
A Fluorescence-based Assay for Measuring Phospholipid Scramblase Activity in Giant Unilamellar Vesicles [PDF]
Bio-Protocol, 2022 Transbilayer movement of phospholipids in biological membranes is mediated by a diverse set of lipid transporters. Among them are scramblases that facilitate rapid bi-directional movement of lipids without metabolic energy input.
Patricia Mathiassen +1 more
doaj +2 more sources
Curcumin–Induced Stabilization of Protein–Based Nano-Delivery Vehicles Reduces Disruption of Zwitterionic Giant Unilamellar Vesicles [PDF]
Molecules, 2022 Curcumin-loaded native and succinylated pea protein nanoparticles, as well as zwitterionic giant unilamellar vesicles were used in this study as model bioactive compound loaded-nanoparticles and biomembranes, respectively, to assess bio-nano interactions.
Ogadimma D. Okagu +2 more
doaj +2 more sources
Effects of membrane potentials on the electroporation of giant unilamellar vesicles. [PDF]
PLoS ONE, 2023 Living organisms maintain a resting membrane potential, which plays an important role in various biophysical and biological processes. In the context of medical applications, irreversible electroporation (IRE) is a non-thermal and minimally invasive ...
Md Abdul Wadud +3 more
doaj +2 more sources
Surfactant-free production of biomimetic giant unilamellar vesicles using PDMS-based microfluidics [PDF]
Communications Chemistry, 2021 The production of giant unilamellar vesicles by microfluidics commonly involves additives, which may interfere with the resultant membrane properties. Here pure lipid GUVs are prepared which exclude residual surfactants and other additives.
Naresh Yandrapalli +3 more
doaj +2 more sources
A Practical Guide to Preparation and Applications of Giant Unilamellar Vesicles Formed via Centrifugation of Water-in-Oil Emulsion Droplets [PDF]
Membranes, 2023 Giant vesicles (GVs), which are closed lipid bilayer membranes with a diameter of more than 1 μm, have attracted attention not only as model cell membranes but also for the construction of artificial cells.
Yiting Zhang, Haruto Obuchi, Taro Toyota
doaj +2 more sources