Engineering Phosphatidylserine Containing Asymmetric Giant Unilamellar Vesicles [PDF]
MembranesThe plasma membrane lipid distribution is asymmetric, with several anionic lipid species located in its inner leaflet. Among these, phosphatidylserine (PS) plays a crucial role in various important physiological functions.
Jake McDonough +5 more
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Light-Switchable Membrane Permeability in Giant Unilamellar Vesicles [PDF]
Pharmaceutics, 2022 In this work, giant unilamellar vesicles (GUVs) were synthesized by blending the natural phospholipid 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) with a photoswitchable amphiphile (1) that undergoes photoisomerization upon irradiation with UV-
Paola Albanese +6 more
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Invagination of Giant Unilamellar Vesicles upon Membrane Mixing with Native Vesicles [PDF]
ACS OmegaWe demonstrate rapid membrane mixing between GUVs of pure lipid compositions and membrane vesicles (MVs) isolated from the plasma membrane of Vero cells, resulting in the transfer of native lipids and proteins to the GUVs. The steps involved in the membrane mixing are docking followed by membrane fusion.
Garvita Dhanawat +3 more
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A Fluorescence-based Assay for Measuring Phospholipid Scramblase Activity in Giant Unilamellar Vesicles [PDF]
Bio-Protocol, 2022 Transbilayer movement of phospholipids in biological membranes is mediated by a diverse set of lipid transporters. Among them are scramblases that facilitate rapid bi-directional movement of lipids without metabolic energy input.
Patricia Mathiassen +1 more
doaj +2 more sources
Curcumin–Induced Stabilization of Protein–Based Nano-Delivery Vehicles Reduces Disruption of Zwitterionic Giant Unilamellar Vesicles [PDF]
Molecules, 2022 Curcumin-loaded native and succinylated pea protein nanoparticles, as well as zwitterionic giant unilamellar vesicles were used in this study as model bioactive compound loaded-nanoparticles and biomembranes, respectively, to assess bio-nano interactions.
Ogadimma D. Okagu +2 more
doaj +2 more sources
Formation of Giant Unilamellar Vesicles Assisted by Fluorinated Nanoparticles [PDF]
Advanced Science, 2023 In the quest to produce artificial cells, one key challenge that remains to be solved is the recreation of a complex cellular membrane. Among the existing models, giant unilamellar vesicles (GUVs) are particularly interesting due to their intrinsic ...
Jorik Waeterschoot +5 more
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Division and Regrowth of Phase-Separated Giant Unilamellar Vesicles*. [PDF]
Angew Chem Int Ed Engl, 2021 AbstractSuccess in the bottom‐up assembly of synthetic cells will depend on strategies for the division of protocellular compartments. Here, we describe the controlled division of phase‐separated giant unilamellar lipid vesicles (GUVs). We derive an analytical model based on the vesicle geometry, which makes four quantitative predictions that we verify
Dreher Y +4 more
europepmc +7 more sources
Effects of membrane potentials on the electroporation of giant unilamellar vesicles. [PDF]
PLoS ONE, 2023 Living organisms maintain a resting membrane potential, which plays an important role in various biophysical and biological processes. In the context of medical applications, irreversible electroporation (IRE) is a non-thermal and minimally invasive ...
Md Abdul Wadud +3 more
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Surfactant-free production of biomimetic giant unilamellar vesicles using PDMS-based microfluidics [PDF]
Communications Chemistry, 2021 The production of giant unilamellar vesicles by microfluidics commonly involves additives, which may interfere with the resultant membrane properties. Here pure lipid GUVs are prepared which exclude residual surfactants and other additives.
Naresh Yandrapalli +3 more
doaj +2 more sources
Measuring Encapsulation Efficiency in Cell-Mimicking Giant Unilamellar Vesicles. [PDF]
ACS Synth Biol, 2023 One of the main drivers within the field of bottom-up synthetic biology is to develop artificial chemical machines, perhaps even living systems, that have programmable functionality. Numerous toolkits exist to generate giant unilamellar vesicle-based artificial cells.
Supramaniam P +7 more
europepmc +5 more sources