Results 101 to 110 of about 40,389 (237)

Thermally Driven Dynamic Behaviors in Polymeric Vesicles

Small, EarlyView.
Synthetic cells aim to mimic biological form, function, and behavior. In this article, the thermoresponsive properties of the polymer poly(ethylene oxide)‐poly(butylene oxide) are leveraged to create a range of polymeric synthetic cell systems on the nanoscale and microscale which possess a range of biomimetic properties, including cargo release ...
Matthew E. Allen, Yeyang Sun, Chi Long Chan, Miguel Paez‐Perez, Oscar Ces, Yuval Elani, Claudia Contini   +6 more
wiley   +1 more source

Bottom-up assembly of functional intracellular synthetic organelles by droplet-based microfluidics

, 2020
Bottom-up synthetic biology has directed most efforts toward the construction of artificial compartmentalized systems that recreate living cell functions in their mechanical, morphological, or metabolic characteristics.
Platzman, I., Schröter, M., Spatz, J., Staufer, O.   +3 more
core   +1 more source

High-speed imaging of giant unilamellar vesicle formation in cDICE

ACS Omega, 2023
AbstractGiant unilamellar vesicles (GUVs) are widely used asin vitromodel membranes in biophysics and as cell-sized containers in synthetic biology. Despite their ubiquitous use, there is no one-size-fits-all method for their production. Numerous methods have been developed to meet the demanding requirements of reproducibility, reliability, and high ...
Lori Van de Cauter, Yash K. Jawale, Daniel Tam, Lucia Baldauf, Lennard van Buren, Gijsje H. Koenderink, Marileen Dogterom, Kristina A. Ganzinger   +7 more
openaire   +3 more sources

Nuclear Assembly in Giant Unilamellar Vesicles Encapsulating Xenopus Egg Extract

Small, EarlyView.
Cell nuclei have been reconstituted in membrane‐less compartments using Xenopus egg extract, but not within lipid bilayer‐enclosed compartments. This study modifies the inverted emulsion method by adding chloroform to the lipid‐dispersed oil and extending lipid monolayer formation at the oil–egg extract interface, enabling the encapsulation of egg ...
Sho Takamori, Hisatoshi Mimura, Toshihisa Osaki, Tomo Kondo, Miyuki Shintomi, Keishi Shintomi, Miho Ohsugi, Shoji Takeuchi   +7 more
wiley   +1 more source

Influenza A matrix protein M1 induces lipid membrane deformation via protein multimerization [PDF]

, 2019
The matrix protein M1 of the Influenza A virus (IAV) is supposed to mediate viral assembly and budding at the plasma membrane (PM) of infected cells. In order for a new viral particle to form, the PM lipid bilayer has to bend into a vesicle toward the ...
Chiantia, Salvatore, Dahmani, Ismail, Ludwig, Kai   +2 more
core   +1 more source

Polymeric Giant Unilamellar Vesicles Support Longevity of Native Nuclei in Protocells

Small Science, EarlyView.
Native nuclei are isolated and incorporated into polymeric giant unilamellar vesicles (pGUVs) using microfluidics. Nucleated pGUVs retain nuclear integrity and function, providing a new platform to study the import process of peptide‐based multicompartment micelles via nuclear localization signals. This bottom‐up chimeric system lays the groundwork for
Lukas Heuberger, Arianna Balestri, Shabnam Tarvirdipour, Larisa E. Kapinos, Roderick Y. H. Lim, Emanuel Lörtscher, Cora‐Ann Schoenenberger, Cornelia G. Palivan   +7 more
wiley   +1 more source

Hydrophobic gating of mechanosensitive channel of large conductance evidenced by single-subunit resolution [PDF]

, 2012
Mechanosensitive (MS) ion channels are membrane proteins that detect and respond to membrane tension in all branches of life. In bacteria, MS channels prevent cells from lysing upon sudden hypoosmotic shock by opening and releasing solutes and water ...
A. Kocer, Anishkin, B. Poolman, Beckstein, Bilston, Blount, Blount, Chang, Colombo, Cruickshank, Dudia, Girard, Gullingsrud, Guzman, Hamill, J. P. Birkner, Kocer, Kyte, Le Dain, Liu, Ou, Perozo, Perozo, Petrov, Powell, Shapovalov, Sukharev, Sukharev, Sukharev, Yoshimura, Yoshimura   +30 more
core   +2 more sources

Phase separation dependent active motion of Janus lipid vesicles [PDF]

arXiv, 2023
Active colloidal systems have emerged as promising contenders for the future of microdevices. While conventional designs have extensively exploited the use of hard colloids, the advancement of cell-inspired architectures represents a pivotal path towards realizing self-regulating and highly functional artificial microswimmers.
arxiv  

Investigating Impacts of Amino Acids on the Structural Stability of Anionic Biomembranes

ChemSystemsChem, Volume 7, Issue 2, March 2025.
Glycine and lysine interact with the anionic membrane composed of POPC and POPG (1 : 1). Lysine partitions into the membrane and induces structural alterations, while the membrane remains impermeable to it. In contrast, glycine causes no physicochemical damage and permeates through the membrane.
Arslan Siddique, Lauren A. Lowe, Soumya Kanti De, Daniel W. K. Loo, Anton P. Le Brun, Andrew R. J. Nelson, Anna Wang   +6 more
wiley   +1 more source