PoET: automated approach for measuring pore edge tension in giant unilamellar vesicles. [PDF]
Leomil FSC +3 more
europepmc +2 more sources
Surfactant-free production of biomimetic giant unilamellar vesicles using PDMS-based microfluidics. [PDF]
The production of giant unilamellar vesicles by microfluidics commonly involves additives, which may interfere with the resultant membrane properties. Here pure lipid GUVs are prepared which exclude residual surfactants and other additives.
Yandrapalli N +3 more
europepmc +2 more sources
Engineering Phosphatidylserine Containing Asymmetric Giant Unilamellar Vesicles. [PDF]
The plasma membrane lipid distribution is asymmetric, with several anionic lipid species located in its inner leaflet. Among these, phosphatidylserine (PS) plays a crucial role in various important physiological functions.
McDonough J +5 more
europepmc +2 more sources
Effects of membrane potentials on the electroporation of giant unilamellar vesicles. [PDF]
Living organisms maintain a resting membrane potential, which plays an important role in various biophysical and biological processes. In the context of medical applications, irreversible electroporation (IRE) is a non-thermal and minimally invasive ...
Wadud MA +3 more
europepmc +2 more sources
Intelligent fluorescence image analysis of giant unilamellar vesicles using convolutional neural network. [PDF]
Background Fluorescence image analysis in biochemical science often involves the complex tasks of identifying samples for analysis and calculating the desired information from the intensity traces.
Lee IH +4 more
europepmc +2 more sources
A Practical Guide to Preparation and Applications of Giant Unilamellar Vesicles Formed via Centrifugation of Water-in-Oil Emulsion Droplets. [PDF]
Giant vesicles (GVs), which are closed lipid bilayer membranes with a diameter of more than 1 μm, have attracted attention not only as model cell membranes but also for the construction of artificial cells.
Zhang Y, Obuchi H, Toyota T.
europepmc +2 more sources
In Situ Assembly of Transmembrane Proteins from Expressed and Synthetic Components in Giant Unilamellar Vesicles. [PDF]
Podolsky KA +4 more
europepmc +3 more sources
Hierarchical unilamellar vesicles of controlled compositional heterogeneity. [PDF]
Eukaryotic life contains hierarchical vesicular architectures (i.e. organelles) that are crucial for material production and trafficking, information storage and access, as well as energy production. In order to perform specific tasks, these compartments
Maik Hadorn +3 more
doaj +1 more source
Visualizing Loss of Plasma Membrane Lipid Asymmetry Using Annexin V Staining
Loss of plasma membrane lipid asymmetry contributes to many cellular functions and responses, including apoptosis, blood coagulation, and cell fusion.
Julia Baum +2 more
doaj +1 more source
Invagination of Giant Unilamellar Vesicles upon Membrane Mixing with Native Vesicles. [PDF]
Dhanawat G, Dey M, Singh A, Parveen N.
europepmc +2 more sources

