Results 31 to 40 of about 4,800 (112)
High-speed imaging of giant unilamellar vesicle formation in cDICE
ACS Omega, 2023 AbstractGiant unilamellar vesicles (GUVs) are widely used asin vitromodel membranes in biophysics and as cell-sized containers in synthetic biology. Despite their ubiquitous use, there is no one-size-fits-all method for their production. Numerous methods have been developed to meet the demanding requirements of reproducibility, reliability, and high ...
Lori Van de Cauter +7 more
openaire +3 more sources
Nuclear Assembly in Giant Unilamellar Vesicles Encapsulating Xenopus Egg Extract
Small, EarlyView.Cell nuclei have been reconstituted in membrane‐less compartments using Xenopus egg extract, but not within lipid bilayer‐enclosed compartments. This study modifies the inverted emulsion method by adding chloroform to the lipid‐dispersed oil and extending lipid monolayer formation at the oil–egg extract interface, enabling the encapsulation of egg ...
Sho Takamori +7 more
wiley +1 more source
Polymeric Giant Unilamellar Vesicles Support Longevity of Native Nuclei in Protocells
Small Science, EarlyView.Native nuclei are isolated and incorporated into polymeric giant unilamellar vesicles (pGUVs) using microfluidics. Nucleated pGUVs retain nuclear integrity and function, providing a new platform to study the import process of peptide‐based multicompartment micelles via nuclear localization signals. This bottom‐up chimeric system lays the groundwork for
Lukas Heuberger +7 more
wiley +1 more source
Investigating Impacts of Amino Acids on the Structural Stability of Anionic Biomembranes
ChemSystemsChem, Volume 7, Issue 2, March 2025.Glycine and lysine interact with the anionic membrane composed of POPC and POPG (1 : 1). Lysine partitions into the membrane and induces structural alterations, while the membrane remains impermeable to it. In contrast, glycine causes no physicochemical damage and permeates through the membrane.
Arslan Siddique +6 more
wiley +1 more source
Visualization and Quantification of Transmembrane Ion Transport into Giant Unilamellar Vesicles [PDF]
Angewandte Chemie International Edition, 2014 AbstractTransmembrane ion transporters (ionophores) are widely investigated as supramolecular agents with potential for biological activity. Tests are usually performed in synthetic membranes that are assembled into large unilamellar vesicles (LUVs). However transport must be followed through bulk properties of the vesicle suspension, because LUVs are ...
Valkenier, H. +3 more
openaire +7 more sources
ChemBioChem, Volume 26, Issue 11, June 3, 2025.The antifungal behavior of artificial palmitoylated or cholesterylated chitinase domain is studied from biological, chemical, and physical properties, highlighting the importance of the chemical properties of the lipid moiety covalently attached to chitinase domain to obtain effective antifungal activity by artificial protein lipidation through a ...
Muhammad Safaat +7 more
wiley +1 more source
Lipid Lateral Organization on Giant Unilamellar Vesicles Containing Lipopolysaccharides [PDF]
Biophysical Journal, 2011 We developed a new (to our knowledge) protocol to generate giant unilamellar vesicles (GUVs) composed of mixtures of single lipopolysaccharide (LPS) species and Escherichia coli polar lipid extracts. Four different LPSs that differed in the size of the polar headgroup (i.e., LPS smooth > LPS-Ra > LPS-Rc > LPS-Rd) were selected to generate GUVs composed
Kubiak, Jakub +3 more
openaire +5 more sources
Advanced Materials, Volume 37, Issue 22, June 5, 2025.Protein can undergo liquid–liquid phase separation and liquid‐to‐solid transition to form liquid condensates and solid aggregates. These phase transitions can be influenced by post‐translational modifications, mutations, and various environmental factors.
Tianchen Li +3 more
wiley +1 more source
Protein Science, Volume 34, Issue 6, June 2025.Abstract Mitochondrial dynamics are regulated by coordinated fission and fusion events that rely on key proteins and lipids organized spatially within the mitochondria. The dynamin‐related GTPase Optic Atrophy 1 (OPA1) is essential for inner mitochondrial membrane fusion and cristae structure maintenance.
Javaid Jabbar +4 more
wiley +1 more source
Angewandte Chemie, Volume 137, Issue 22, May 26, 2025.Ein biomimetisches Lipiddoppelschichtmembran‐System ermöglicht einen lichtinduzierten Elektronentransfer durch die Membran unter sowohl aeroben (sauerstoffreichen) als auch anaeroben (sauerstoffarmen) Bedingungen. Der Elektronentransfer wird durch einen organischen Chromophor vermittelt – ein Molekül, das symmetrisch aufgebaut und leicht zu ...
Novitasari Sinambela +4 more
wiley +1 more source