A Note on Vestigial Osmotic Pressure
Membranes, 2023 Recent experiments have indicated that at least a part of the osmotic pressure across the giant unilamellar vesicle (GUV) membrane was balanced by the rapid formation of the monodisperse daughter vesicles inside the GUVs through an endocytosis-like ...
Hao Wu +2 more
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Interplay of packing and flip-flop in local bilayer deformation. How phosphatidylglycerol could rescue mitochondrial function in a cardiolipin-deficient yeast mutant [PDF]
Biophys. J. 107(4): 879-890 (2014), 2014 In a previous work, we have shown that a spatially localized transmembrane pH gradient, produced by acid micro-injection near the external side of cardiolipin-containing giant unilamellar vesicles, leads to the formation of tubules that retract after the dissipation of this gradient. These tubules have morphologies similar to mitochondrial cristae. The
arxiv +1 more source
Membrane sculpting by curved DNA origami scaffolds
Nature Communications, 2018 BAR domain proteins feature a “banana-like” shape which is thought to aid membrane scaffolding and membrane tubulation. Here authors use DNA origami mimicking BAR domains, giant unilamellar vesicles and fluorescence imaging to study how different BAR ...
Henri G. Franquelim +4 more
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Asymmetric Oxidation of Giant Vesicles triggers Curvature-associated Shape Transition and Permeabilization. Running title: Oxidation of Vesicles produces Curvature [PDF]
Biophysical Journal, Biophysical Society, 2009, 97 (11),
pp.2904-2912, 2019 (200 words) Oxidation of unsaturated lipids is a fundamental process involved in cell bioenergetics as well as in cell death. Using giant unilamellar vesicles and a chlorin photosensitizer, we asymmetrically oxidized the outer or inner monolayers of lipid membranes.
arxiv +1 more source
Deciphering the Assembly of Enveloped Viruses Using Model Lipid Membranes
Membranes, 2022 The cell plasma membrane is mainly composed of phospholipids, cholesterol and embedded proteins, presenting a complex interface with the environment.
Erwan Brémaud +2 more
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Visualization of membrane loss during the shrinkage of giant vesicles under electropulsation [PDF]
Biophysical Journal 96, pp 4109-4121 (2009), 2008 We study the effect of permeabilizing electric fields applied to two different types of giant unilamellar vesicles, the first formed from EggPC lipids and the second formed from DOPC lipids. Experiments on vesicles of both lipid types show a decrease in vesicle radius which is interpreted as being due to lipid loss during the permeabilization process ...
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Entry of Microparticles into Giant Lipid Vesicles by Optical Tweezers [PDF]
, 2023 Entry of micro- or nano-sized objects into cells or vesicles made of lipid membranes occur in many processes such as entry of viruses in host cells, microplastics pollution, drug delivery or biomedical imaging. Here, we investigated the microparticle crossing of lipid membranes in giant unilamellar vesicles in the absence of strong binding interactions
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Permeability of phospholipid membrane for small polar molecules determined from osmotic swelling of giant phospholipid vesicles [PDF]
Advances in Planar Lipid Bilayers and Liposomes, Volume 16, 2012,
Pages 301-335, 2012 A method for determining permeability of phospholipid bilayer based on the osmotic swelling of micrometer-sized giant unilamellar vesicles (GUVs) is presented as an alternative to the two established techniques, dynamic light scattering on liposome suspension, and electrical measurements on planar lipid bilayers.
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Scientific Reports, 2018 Super-resolution imaging and single-particle tracking require cells to be immobile as any movement reduces the resolution of the measurements. Here, we present a method based on APTES-glutaraldehyde coating of glass surfaces to immobilize cells without ...
Łukasz Syga +3 more
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Relaxation of a Simulated Lipid Bilayer Vesicle Compressed by an AFM [PDF]
, 2016 Using Coarse-Grained Molecular Dynamics simulations, we study the relaxation of bilayer vesicles, uniaxially compressed by an Atomic Force Microscope (AFM) cantilever. The relaxation time exhibits a strong force-dependence. Force-compression curves are very similar to recent experiments wherein giant unilamellar vesicles were compressed in a nearly ...
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