Intra- and Interspecies Analyses of the Carcinoembryonic Antigen (CEA) Gene Family Reveal Independent Evolution in Primates and Rodents [PDF]
, 1989 Various rodent and primate DNAs exhibit a stronger intra- than interspecies cross-hybridization with probes derived from the N-terminal domain exons of human and rat carcinoembryonic antigen (CEA)-like genes.
Rudert, Fritz +2 more
core +1 more source
Making Molecular Diagnostics Faster
International Journal of Laboratory Hematology, EarlyView.ABSTRACT Background Over the past 40 years, molecular diagnostic methods have evolved from multi‐step, time‐consuming protocols towards either rapid targeted tests or expansive, massively parallel testing. Aims Here we consider the speed limits of targeted molecular diagnostics, considering the three sequential required steps: nucleic acid preparation,
Carl T. Wittwer +5 more
wiley +1 more source
Mechanistic Insight into the Enzymatic Reduction of Truncated Hemoglobin N of Mycobacterium tuberculosis: role of the CD loop and pre-A Motif in electron cycling [PDF]
, 2014 Background: The HbN of Mycobacterium tuberculosis carries a potent nitric-oxide dioxygenase activity despite lacking a reductase domain. Results: The NADH-ferredoxin reductase system acts as an efficient partner for the reduction of HbN.
Bidon Chanal, Axel +12 more
core +2 more sources
Molecular Testing in Sickle Cell Disease: From Newborn Screening to Transfusion Care
International Journal of Laboratory Hematology, EarlyView.ABSTRACT Sickle cell disease (SCD) is one of the most frequent monogenic diseases worldwide and a highly heterogeneous and complex disease. SCD care carries several challenges. This includes early and accurate diagnosis as well as optimal red blood cell transfusion matching in this population carrying a high risk of alloimmunization.
Thomas Pincez, Yves D. Pastore
wiley +1 more source
International Journal of Laboratory Hematology, EarlyView.ABSTRACT Background Accurate classification of novel globin gene variants is critical for the diagnosis and management of thalassaemia. The adaptation of ACMG/AMP guidelines for globin genes represents an important step toward standardising variant interpretation and enhancing clinical utility in the field.
Norafiza Mohd Yasin +14 more
wiley +1 more source
Haematologica, 2014 A major barrier to the clinical use of erythrocytes generated in vitro from pluripotent stem cells or cord blood progenitors is failure of these erythrocytes to express adult hemoglobin.
Kongtana Trakarnsanga +9 more
doaj +1 more source
Genotoxic Potential of Lineage-specific Lentivirus Vectors Carrying the β-Globin Locus Control Region [PDF]
, 2009 Paritha Arumugam +9 more
openalex +1 more source
A Feedback Loop Consisting of MicroRNA 23a/27a and the β-Like Globin Suppressors KLF3 and SP1 Regulates Globin Gene Expression [PDF]
, 2013 Yanni Ma +17 more
openalex +1 more source
Hamster leukemia virus: lack of endogenous DNA synthesis and unique structure of its DNA polymerase [PDF]
, 1974 Infectious hamster leukemia virus (HaLV) contains a DNA polymerase different from those of murine and avian viruses. No endogenous reaction directed by the 60 to 70S RNA of HaLV could be demonstrated in detergenttreated HaLV virions, nor could the ...
Baltimore, David +3 more
core
Plant Biotechnology Journal, EarlyView.ABSTRACT The coupling of replication and encapsidation in the bipartite plant virus, cowpea mosaic virus (CPMV), has been used to specifically incorporate designer RNA molecules into viral particles via transient expression in Nicotiana benthamiana. This is achieved by placing the desired RNA sequence between the 5′ and 3′ UTRs of CPMV RNA‐2 so that ...
H. Peyret +5 more
wiley +1 more source