Results 31 to 40 of about 173,792 (325)

TMEM8 – a non-globin gene entrapped in the globin web [PDF]

Nucleic Acids Research, 2009
For more than 30 years it was believed that globin gene domains included only genes encoding globin chains. Here we show that in chickens, the domain of alpha-globin genes also harbor the non-globin gene TMEM8. It was relocated to the vicinity of the alpha-globin cluster due to inversion of an approximately 170-kb genomic fragment.
Elena S. Philonenko, Olga V. Iarovaia, Denis B. Klochkov, Sergey V. Razin, V. V. Borunova, Alexey A. Gavrilov   +5 more
openaire   +3 more sources

Editing a γ-globin repressor binding site restores fetal hemoglobin synthesis and corrects the sickle cell disease phenotype

Science Advances, 2020
Editing the fetal γ-globin promoters in hematopoietic stem cells from sickle cell disease patients induces therapeutic γ-globin levels. Sickle cell disease (SCD) is caused by a single amino acid change in the adult hemoglobin (Hb) β chain that causes Hb ...
Leslie Weber, G. Frati, T. Felix, G. Hardouin, Antonio Casini, Clara Wollenschlaeger, Vasco Meneghini, C. Masson, A. de Cian, A. Chalumeau, F. Mavilio, Mario Amendola, I. André-Schmutz, A. Cereseto, W. El Nemer, J. Concordet, C. Giovannangeli, M. Cavazzana, A. Miccio   +18 more
semanticscholar   +1 more source

Histone crosstalk directed by H2B ubiquitination is required for chromatin boundary integrity [PDF]

, 2011
Genomic maps of chromatin modifications have provided evidence for the partitioning of genomes into domains of distinct chromatin states, which assist coordinated gene regulation.
Hair, A., Heath, C., Ma, M.K-W., West, A.   +3 more
core   +10 more sources

Characterization of N-Terminal Acetylated α-Hemoglobin Stabilizing Protein (AHSP) by Top-Down High-Resolution Mass Spectrometry From Human Preterm Newborns Oral Fluid. [PDF]

Rapid Commun Mass Spectrom
ABSTRACT Rationale Alpha‐hemoglobin stabilizing protein (AHSP) is an erythroid‐specific protein forming a stable complex with free α‐hemoglobin, but not with β‐hemoglobin or hemoglobin A (α2β2), thus preventing harmful aggregation of α‐hemoglobin during normal erythroid cell development and avoiding its pro‐oxidant activity.
Iavarone F, Tirone C, Fattore S, De Tomaso D, Menzella N, Vento G, Olianas A, Manconi B, Cabras T, Guadalupi G, Contini C, Boroumand M, Desiderio C, Muntiu A, Fiorita A, Fraschini M, Fanos V, Faa G, Messana I, Castagnola M.   +19 more
europepmc   +2 more sources

The folding pathway for globins [PDF]

FEBS Letters, 1977
Previous investigations have successfully predicted the three-dimensional structures of trypsin inhibitor [ 1,2] , myoglobin [3,4] , TMV protein [ 51 and rubredoxin [2]. Using mechanisms of protein chain folding which one of us [5 $1 suggested,.we can find a common folding pathway for the globins which are included in the ‘Atlas of Protein Sequence and
V.I. Lim, Alexander V. Efimov
openaire   +3 more sources

A simple, highly efficient method for heterologous expression in mammalian primary neurons using cationic lipid-mediated mRNA transfection

Frontiers in Neuroscience, 2010
Expression of heterologous proteins in adult mammalian neurons is a valuable technique for the study of neuronal function. The postmitotic nature of mature neurons prevents effective DNA transfection using simple, cationic lipid-based methods.
Damian J Williams, Henry L Puhl, Stephen R Ikeda   +2 more
doaj   +1 more source

TFII-I/Gtf2i and Erythro-Megakaryopoiesis

Frontiers in Physiology, 2020
TFII-I is a ubiquitously expressed transcription factor that positively or negatively regulates gene expression. TFII-I has been implicated in neuronal and immunologic diseases as well as in thymic epithelial cancer.
Aishwarya Gurumurthy, Qiong Wu, Rukiye Nar, Kimberly Paulsen, Alexis Trumbull, Ryan C. Fishman, Marjorie Brand, John Strouboulis, Zhijian Qian, Jörg Bungert   +9 more
doaj   +1 more source

Analysis of β-globin chromatin micro-environment using a novel 3C variant, 4Cv [PDF]

, 2010
Copyright: © 2010 Pink et al.Higher order chromatin folding is critical to a number of developmental processes, including the regulation of gene expression.
Christopher H Eskiw, Daniel P Caley, David R F Carter, Ryan C Pink, Ryan C Pink   +4 more
core   +3 more sources

Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

Proceedings of the National Academy of Sciences of the United States of America, 1972
A convenient technique for the partial purification of large quantities of functional, poly(adenylic acid)-rich mRNA is described. The method depends upon annealing poly(adenylic acid)-rich mRNA to oligothymidylic acid-cellulose columns and its elution ...
H. Aviv, P. Leder
semanticscholar   +1 more source

Genetic and Epigenetic Therapies for β-Thalassaemia by Altering the Expression of α-Globin Gene

Frontiers in Genome Editing, 2021
β-Thalassaemia is caused by over 300 mutations in and around the β-globin gene that lead to impaired synthesis of β-globin. The expression of α-globin continues normally, resulting in an excess of α-globin chains within red blood cells and their ...
Sachith Mettananda, Sachith Mettananda
doaj   +1 more source