Use of Coniothyrium minitans transformed with the hygromycin B resistance gene to study survival and infection of Sclerotinia sclerotiorum sclerotia in soil [PDF]
A Coniothyrium minitans strain (T3) co-transformed with the genes for β-glucuronidase (uidA) and hygromycin phosphotransferase (hph), the latter providing resistance to the antibiotic hygromycin B, was used to investigate the survival and infection of ...
Alison Stewart +25 more
core +1 more source
Continuous Spectrophotometric Assay for β-Glucuronidase
A continuous spectrophotometric assay has been developed for detecting β-glucuronidase activity. In the assay, Para-nitrophenyl β-d-glucuronide is cleaved to yield a chromophoric product. With the commercial E.
Sanjukta Aich +2 more
doaj +1 more source
Amino acid‐vectorized salicylic acid activates PR1/NPR1‐dependent defenses without phytotoxicity, showing an efficient strategy for controlled delivery and enhanced plant immunity. Abstract BACKGROUND Addressing global food security under rapid population growth and climate change requires sustainable strategies to protect crops from pathogens while ...
Ruth Oussou +6 more
wiley +1 more source
In vitro metabolic studies of REV-ERB agonists SR9009 and SR9011 [PDF]
SR9009 and SR9011 are attractive as performance-enhancing substances due to their REV-ERB agonist effects and thus circadian rhythm modulation activity.
Deventer, Koen +4 more
core +3 more sources
TcALS knockdown accelerates metamorphosis via insulin signaling in Tribolium castaneum
RNAi silencing of TcALS accelerates larva‐to‐pupa metamorphosis in Tribolium castaneum. Abstract Insect metamorphosis is an evolutionary adaptation precisely regulated by nutritional and hormonal cues. The red flour beetle Tribolium castaneum, a major stored‐product pest, serves as an excellent model for studying this process.
Min Li +8 more
wiley +1 more source
A neutrophil-dependent pathway for the generation of a neutral peptide mediator: partial characterization of components and control by alpha-1-antitrypsin. [PDF]
A biologically active neutral peptide mediator is cleaved from a plasma protein substrate by an alpha-1-antitrypsin-inhibitable serine protease apparently residing on the membrane of the human neutrophil. The peptide mediator has an approximate mol wt of
Austen, KF, Goetzl, EJ, Wintroub, BU
core
Preliminary evaluation of probiotic properties of Lactobacillus strains isolated from Sardinian dairy products [PDF]
Twenty-three Lactobacillus strains of dairy origin were evaluated for some functional properties relevant to their use as probiotics. A preliminary subtractive screening based on the abilities to inhibit the growth of microbial pathogens and hydrolyze ...
Conti S +7 more
core +1 more source
Untargeted multiomic profiling of cerebrospinal fluid reveals that proteomic, but not lipidomic, signatures robustly distinguish ALS patients from controls and stratify individuals by survival, highlighting marked molecular differences between short survival and long survival disease.
Sergio Roca‐Pereira +19 more
wiley +1 more source
Activity-based probes for functional interrogation of retaining β-glucuronidases [PDF]
Humans express at least two distinct β-glucuronidase enzymes that are involved in disease: exo-acting β-glucuronidase (GUSB), whose deficiency gives rise to mucopolysaccharidosis type VII, and endo-acting heparanase (HPSE), whose overexpression is ...
Aerts, Johannes M.F.G. +14 more
core +2 more sources
Synthesis of Chromen-4-One-Oxadiazole Substituted Analogs as Potent β-Glucuronidase Inhibitors
Chromen-4-one substituted oxadiazole analogs 1–19 have been synthesized, characterized and evaluated for β-glucuronidase inhibition. All analogs exhibited a variable degree of β-glucuronidase inhibitory activity with IC50 values ranging ...
Muhammad Taha +10 more
doaj +1 more source

