Results 61 to 70 of about 43,079 (209)

Upregulation of Jasmonate-Inducible Defense Proteins and Differential Colonization of Roots of Oryza sativa Cultivars with the Endophyte Azoarcus sp.

open access: yesMolecular Plant-Microbe Interactions, 2006
The endophyte Azoarcus sp. strain BH72 expresses nitrogenase (nif) genes inside rice roots. We applied a proteomic approach to dissect responses of rice roots toward bacterial colonization and jasmonic acid (JA) treatment.
Lucie Miché   +4 more
doaj   +1 more source

Native β-glucuronidase electrophoresis and activity assays.

open access: yes, 2015
(A) E. coli β-glucuronidase (0.05, 0.5, 5, and 50 μg) was electrophoresed under native conditions, followed by 30-minute incubation with X-GlcA at room temperature (B). Oligomeric forms of β-glucuronidase are labeled.
Kimberly T. Lane (762232)   +2 more
core   +1 more source

A horizontally transferred bacterial gene for pantothenic acid biosynthesis regulates diapause and reproduction in the spider mite Amphitetranychus viennensis

open access: yesInsect Science, EarlyView.
A bacterial‐derived AvPBL gene encoding a pantothenate‐β‐alanine ligase was identified in Amphitetranychus viennensis. RNAi silencing of AvPBL reduced pantothenic acid production, impairing reproduction and diapause induction, while pantothenic acid supplementation rescued these phenotypes.
Meibin Liu   +8 more
wiley   +1 more source

Phenoxyacetohydrazide Schiff Bases: β-Glucuronidase Inhibitors

open access: yesMolecules, 2014
Phenoxyacetohydrazide Schiff base analogs 1–28 have been synthesized and their in vitro β-glucouoronidase inhibition potential studied. Compounds 1 (IC50 = 9.20 ± 0.32 µM), 5 (IC50 = 9.47 ± 0.16 µM), 7 (IC50 = 14.7 ± 0.19 µM), 8 (IC50 = 15.4 ± 1.56 µM),
Waqas Jamil   +8 more
doaj   +1 more source

Rifaximin ameliorates memory impairment and Alzheimer's pathological changes in APP/PS1 mice associated with changes in the gut microbiome–bile acids–serum metabolites networks

open access: yesBritish Journal of Pharmacology, EarlyView.
Background and Purpose Modifying the brain–gut–microbiota axis has emerged as a promising therapeutic strategy against Alzheimer's disease (AD). Whether rifaximin, a non‐absorbed and non‐systemic antibiotic, can be applied to the treatment of AD remains unexplored.
Di Wu   +7 more
wiley   +1 more source

Investigation of the inhibitory potential of secondary metabolites isolated from Fernandoa adenophylla against Beta-glucuronidase via molecular docking and molecular dynamics simulation studies.

open access: yesPLoS ONE
Elevated β-glucuronidase activity is associated with the production of toxic metabolites that contribute to tumor development and other diseases. Inhibiting this enzyme may offer therapeutic potential, including the prevention of colonic carcinogenesis ...
Abdur Rauf   +11 more
doaj   +1 more source

Continuous Spectrophotometric Assay for β-Glucuronidase

open access: yesBioTechniques, 2001
A continuous spectrophotometric assay has been developed for detecting β-glucuronidase activity. In the assay, Para-nitrophenyl β-d-glucuronide is cleaved to yield a chromophoric product. With the commercial E.
Sanjukta Aich   +2 more
doaj   +1 more source

Vitamin D deficiency in early pregnancy and the risk of developing cerebral palsy: A case–control study

open access: yesDevelopmental Medicine &Child Neurology, EarlyView.
Abstract Aim To investigate whether maternal deficiency of vitamin D in early pregnancy increases the risk of cerebral palsy (CP) in offspring. Method This registry and biobank‐based case–control study consisted of mother–child pairs from Scania, Sweden. Cases (n = 322) were identified from a nationwide CP‐register born between 1995 and 2014.
Ebba Bergman   +3 more
wiley   +1 more source

Cryo‐EM structure of GH43 β‐Xylosidase from Enterobacter cloacae provides insights into substrate specificity and the role of an auxiliary domain in enzymatic activity

open access: yesThe FEBS Journal, EarlyView.
Xylan, the second most abundant plant cell wall polysaccharide, is degraded by β‐xylanases and β‐xylosidases. Here, we present the 2.65 Å cryo‐EM structure of Enterobacter cloacae β‐xylosidase (EcXyl43, GH43) and the 2.4 Å X‐ray structure of its inactive F507A mutant.
Lorenzo Briganti   +8 more
wiley   +1 more source

Crystal structure and biochemical analysis of the polyester‐degrading carboxylesterase SM0281 from the symbiotic legume‐associated rhizobacterium Sinorhizobium (Ensifer) meliloti

open access: yesThe FEBS Journal, EarlyView.
The microbial degradation of synthetic polyesters such as polyethylene terephthalate (PET) is mediated by diverse α/β‐hydrolases, many of which remain poorly characterized. In this study, we investigated the α/β‐hydrolase SM0281 from Sinorhizobium meliloti and found that it exhibits robust carboxylesterase and polyesterase activities toward PET, PCL ...
Sofia Lemak   +7 more
wiley   +1 more source

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