Results 41 to 50 of about 9,046 (297)

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Glutamate transporter control of ambient glutamate levels

Neurochemistry International, 2014
Accurate knowledge of the ambient extracellular glutamate concentration in brain is required for understanding its potential impacts on tonic and phasic receptor signaling. Estimates of ambient glutamate based on microdialysis measurements are generally in the range of ∼2-10μM, approximately 100-fold higher than estimates based on electrophysiological ...
Sun, Weinan, Shchepakin, Denis, Kalachev, Leonid V., Kavanaugh, Michael P.   +3 more
openaire   +2 more sources

Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations

FEBS Letters, EarlyView.
The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas, Brigitta Maruzs, Zsófia E. Kálmán, Tomas Klumpler, Gyula Batta, Bálint Péterfia, Zoltán Gáspári   +6 more
wiley   +1 more source

Cofactor Tail Length Modulates Catalysis of Bacterial F420-Dependent Oxidoreductases

Frontiers in Microbiology, 2017
F420 is a microbial cofactor that mediates a wide range of physiologically important and industrially relevant redox reactions, including in methanogenesis and tetracycline biosynthesis.
Blair Ney, Blair Ney, Carlo R. Carere, Richard Sparling, Richard Sparling, Thanavit Jirapanjawat, Matthew B. Stott, Colin J. Jackson, John G. Oakeshott, Andrew C. Warden, Chris Greening, Chris Greening   +11 more
doaj   +1 more source

The preparation of substituted γ-Benzyl-L-glutamates and β-Benzyl-L-aspartates

, 1965
Substituted γ-benzyl-L-glutamates and β-benzyl-L-aspartates have been obtained from the alkali metal salts of the corresponding amino acid copper complexes and substituted benzyl halides under various experimental conditions.
R Ledger, FHC Stewart
core   +1 more source

An unexpected alternative viologen electron mediator site in tungsten‐containing formate dehydrogenase

FEBS Letters, EarlyView.
An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley   +1 more source

Biochemical characterization of mutants in the active site residues of the β-galactosidase enzyme of Bacillus circulans ATCC 31382

FEBS Open Bio, 2014
The Bacillus circulans ATCC 31382 β-galactosidase (BgaD) is a retaining-type glycosidase of glycoside hydrolase family 2 (GH2). Its commercial enzyme preparation, Biolacta N5, is used for commercial-scale production of galacto-oligosaccharides (GOS). The
Jelle B. Bultema, Bas J.H. Kuipers, Lubbert Dijkhuizen   +2 more
doaj   +1 more source

Electron transfer between complexes III and IV in S. cerevisiae mitochondrial membranes

FEBS Letters, EarlyView.
Mitochondrial oxidative phosphorylation in S. cerevisiae mitoplasts is limited by complex IV catalytic capacity, rather than two‐dimensional cytochrome c diffusion. At physiological cytochrome c : supercomplex ratios at salinity equivalent to that of 20 mm monovalent salt, activity is maximized, indicating that this low ionic strength accurately mimics
Ana Paula Lobez, Mikael Oliveberg, Peter Brzezinski   +2 more
wiley   +1 more source

Long-term NMDAR antagonism correlates reduced astrocytic glutamate uptake with anxiety-like phenotype

Frontiers in Cellular Neuroscience, 2015
The role of glutamate N-methyl-D-Aspartate receptor (NMDAR) hypofunction has been extensively studied in schizophrenia; however, less is known about its role in anxiety disorders.
Eduardo R Zimmer, Vitor R Torrez, Eduardo eKalinine, Eduardo eKalinine, Marina C Augustin, Kamila C Zenki, Roberto Farina Almeida, Gisele eHansel, Alexandre P Muller, Diogo O Souza, Rodrigo eMachado-Vieira, Rodrigo eMachado-Vieira, Luis V Portela   +12 more
doaj   +1 more source

Tumour–host interactions in Drosophila: mechanisms in the tumour micro‐ and macroenvironment

Molecular Oncology, EarlyView.
This review examines how tumour–host crosstalk takes place at multiple levels of biological organisation, from local cell competition and immune crosstalk to organism‐wide metabolic and physiological collapse. Here, we integrate findings from Drosophila melanogaster studies that reveal conserved mechanisms through which tumours hijack host systems to ...
José Teles‐Reis, Tor Erik Rusten
wiley   +1 more source