Linked production of pyroglutamate-modified proteins via self-cleavage of fusion tags with TEV protease and autonomous N-terminal cyclization with glutaminyl cyclase in vivo. [PDF]
PLoS One, 2014 Overproduction of N-terminal pyroglutamate (pGlu)-modified proteins utilizing Escherichia coli or eukaryotic cells is a challenging work owing to the fact that the recombinant proteins need to be recovered by proteolytic removal of fusion tags to expose ...
Shih YP +4 more
europepmc +2 more sources
NeuproGemp, a polyphenol-rich botanical formula, ameliorates Alzheimer's-like pathology in APP/PS1 mice via inhibition of human glutaminyl cyclase. [PDF]
Front PharmacolAlzheimer’s disease (AD) is a progressive neurodegenerative disorder characterized by cognitive decline and amyloid-β (Aβ) accumulation. Increasing evidence suggests that dietary bioactive compounds may modulate neurodegenerative processes.
Tseng TS +6 more
europepmc +2 more sources
Immunohistochemical Evidence from APP-Transgenic Mice for Glutaminyl Cyclase as Drug Target to Diminish pE-Abeta Formation. [PDF]
Molecules, 2018 Oligomeric assemblies of neurotoxic amyloid beta (Abeta) peptides generated by proteolytical processing of the amyloid precursor protein (APP) play a key role in the pathogenesis of Alzheimer’s disease (AD).
Hartlage-Rübsamen M +10 more
europepmc +2 more sources
Glutaminyl cyclase activity correlates with levels of Aβ peptides and mediators of angiogenesis in cerebrospinal fluid of Alzheimer's disease patients. [PDF]
Alzheimers Res Ther, 2017 Background Pyroglutamylation of truncated Aβ peptides, which is catalysed by enzyme glutaminyl cyclase (QC), generates pE-Aβ species with enhanced aggregation propensities and resistance to most amino-peptidases and endo-peptidases.
Bridel C +8 more
europepmc +2 more sources
Disturbed Ca2+ homeostasis increases glutaminyl cyclase expression; connecting two early pathogenic events in Alzheimer's disease in vitro. [PDF]
PLoS One, 2012 A major neuropathological hallmark of Alzheimer's disease (AD) is the deposition of aggregated β amyloid (Aβ) peptide in the senile plaques. Aβ is a peptide of 38-43 amino acids and its accumulation and aggregation plays a key role early in the disease ...
De Kimpe L +5 more
europepmc +2 more sources
Human glutaminyl cyclase: Structure, function, inhibitors and involvement in Alzheimer’s disease
Pharmacological Research, 2019 Human glutaminyl cyclase (hQC) is an important enzyme for post-translational modification by converting the N-terminal glutaminyl and glutamyl into pyroglutamate (pGlu) through cyclization. The two isoforms of hQC, secretory glutaminyl cyclase (sQC) and golgi resident glutaminyl cyclase (gQC), are involved in various pathological conditions especially ...
Kam Y J Zhang
exaly +3 more sources
Novel small molecule targeting PgQC reduces <i>Porphyromonas gingivalis</i> virulence. [PDF]
Front Oral HealthIntroductionPeriodontitis, a chronic inflammatory disease affecting the periodontium, is primarily driven by dysbiosis of the oral microbiome with Porphyromonas gingivalis as a keystone pathogen.
Taudte N +7 more
europepmc +2 more sources
Glutaminyl cyclase-mediated toxicity of pyroglutamate-beta amyloid induces striatal neurodegeneration. [PDF]
BMC Neurosci, 2013 Abstract Background Posttranslational modifications of beta amyloid (Aβ) have been shown to affect its biophysical and neurophysiological properties. One of these modifications is N-terminal pyroglutamate (pE) formation.
Becker A +11 more
europepmc +5 more sources
In vitro and in silico determination of glutaminyl cyclase inhibitors. [PDF]
RSC Adv, 2019 Rational design of new hQC inhibitors.
Tran PT +5 more
europepmc +4 more sources
In Situ Detection Method for Glutaminyl Cyclase Activity in Polyacrylamide Gels
BioTechniques, 1998 Justin B. Houseknecht +2 more
doaj +3 more sources