Results 31 to 40 of about 32,114 (276)

Stabilizing Functional Nucleic Acids for Real‐Sample Diagnostics

Angewandte Chemie, EarlyView.
Functional nucleic acids enable programmable real‐sample diagnostics, but often lose function in complex biological matrices. This review highlights four stabilization levers—chemical, topological, architectural, and microenvironment/interface engineering—that preserve aptamer and DNAzyme performance in saliva, serum, urine, and stool, reframing ...
Soyeon Lee, Letizia Dondi, Amal Mathai, Connor Nurmi, Yingfu Li   +4 more
wiley   +2 more sources

Immune response of amebiasis and immune evasion by Entamoeba histolytica

Frontiers in Immunology, 2016
Entamoeba histolytica is a protozoan parasite and the causative agent of amebiasis. It is estimated approximately 1% of humans are infected with E. histolytica, resulting in an estimate of 100,000 deaths annually.
Kumiko eNakada-Tsukui, Tomoyoshi eNozaki, Tomoyoshi eNozaki   +2 more
doaj   +1 more source

Carbohydrate-Modified Electrode Surfaces Sensitive to ß-Glucosidase Enzyme Activity

International Journal of Electrochemical Science, 2014
A surface modification which renders a carbon electrode sensitive to β-glucosidase enzyme activity is reported. 2-Nitro-4-(4-diazophenoxymethyl)-phenyl β-D-glucopyranoside was prepared in four steps from the known starting material 2-nitro-4-benzyloxy 2 ...
Emily M. Ervin, Benjamin T. Adams, Nadine Snyman, Christopher P. Phenix, James K. Kariuki, Brian P. Rempel   +5 more
doaj   +1 more source

An Epoxide Intermediate in Glycosidase Catalysis [PDF]

ACS Central Science, 2020
Retaining glycoside hydrolases cleave their substrates through stereochemical retention at the anomeric position. Typically, this involves two-step mechanisms using either an enzymatic nucleophile via a covalent glycosyl enzyme intermediate, or neighboring group participation by a substrate-borne 2-acetamido neighboring group via an ...
Lukasz F. Sobala, Gaetano Speciale, Sha Zhu, Lluı́s Raich, Natalia Sannikova, Andrew J. Thompson, Zalihe Hakki, Dan Lu, Saeideh Shamsi Kazem Abadi, Andrew R. Lewis, Vı́ctor Rojas-Cervellera, Ganeko Bernardo-Seisdedos, Yongmin Zhang, Oscar Millet, Jesús Jiménez-Barbero, Andrew J. Bennet, Matthieu Sollogoub, Carme Rovira, Gideon J. Davies, Spencer J. Williams   +19 more
doaj   +9 more sources

From Covalent Glycosidase Inhibitors to Activity‐Based Glycosidase Probes

Chemistry – A European Journal, 2014
AbstractActivity‐based protein profiling has emerged as a powerful discovery tool in chemical biology and medicinal chemistry research. Success of activity‐based protein profiling hinges on the presence of compounds that can covalently and irreversibly bind to enzymes, do so selectively in the context of complex biological samples, and subsequently ...
Willems, Lianne I., Jiang, Jianbing, Li, Kah-Yee, Witte, Martin D., Kallemeijn, Wouter W., Beenakker, Thomas J. N., Schröder, Sybrin P., Aerts, Johannes M. F. G., van der Marel, Gijsbert A., Codée, Jeroen D. C., Overkleeft, Hermen S.   +10 more
openaire   +4 more sources

Effects of some phenolic compounds on the inhibition of α-glycosidase enzyme-immobilized on Pluronic®F127 micelles: An in vitro and in silico study

, 2021
This study aimed to consider the activity of immobilized alpha-glycosidase, significant enzyme defined in serum glucose adjustment, on Pluronic (R) F127 micelles and to compare the results with theoretical molecular docking studies.
Özel, Şahin, Gür, Bahri, Taslimi, Parham, Kassa, Solomon Bezabeh, Gülçin, İlhami, Önganer, Yavuz, Solomon Bezabeh Kassa, Yavuz Onganer, Bahri Gür, İlhami Gülçin, Şahin Özel, Parham Taslimi   +11 more
core   +2 more sources

Effects of long-term fertilization on glycosidase and phosphomonoesterase activities in black soil

浙江大学学报. 农业与生命科学版, 2008
Surface soil (0-20 cm) samples were collected from a 25 years fertilization experimental field to study the effects of different fertilization practices on the activities of glycosidase (α-, β-glucosidase and α-, β-galactosidase) and acid-and alkaline ...
MA Xing-zhu, CHEN Li-jun, WU Zhi-jie, ZHANG Li-li   +3 more
doaj   +1 more source

Automated assay of glycosidases [PDF]

Analytical Biochemistry, 1978
The Technicon Basic Auto Analyzer sampler system was modified for simultaneous sampling of glycosidase(s) and substrate-buffer solutions. The inexpensive modification allows performance of automated enzyme analyses and enzyme kinetic studies with minimal consumption of substrate and/or enzyme.
Sarber, Robert, Langnas, Alan N., Jourdian, George W.   +2 more
openaire   +3 more sources

Detection of Bacterial α-l-Fucosidases with an Ortho-Quinone Methide-Based Probe and Mapping of the Probe-Protein Adducts

Molecules, 2022
Fucosidases are associated with several pathological conditions and play an important role in the health of the human gut. For example, fucosidases have been shown to be indicators and/or involved in hepatocellular carcinoma, breast cancer, and ...
Yvette M. C. A. Luijkx, Anniek J. Henselijn, Gerlof P. Bosman, Dario A. T. Cramer, Koen C. A. P. Giesbers, Esther M. van ‘t Veld, Geert-Jan Boons, Albert J. R. Heck, Karli R. Reiding, Karin Strijbis, Tom Wennekes   +10 more
doaj   +1 more source

Synthesis of α-glycosidase hybrid nano-flowers and their application for enriching and screening α-glycosidase inhibitors

, 2018
To rapidly and accurately screen compounds present in traditional Chinese herbal medicines for α-glycosidase inhibitors, we synthesized a novel nano-affinity material to enable the targeted screening of α-glycosidase inhibitors.
Yuanyuan Zhang, Xiao-Ying Yin, Xi Wan, Jieming Liu, Shuting Gao, Weiting Liu, Han Wang, Kang Qian, Qing-Shan Liu   +8 more
core   +1 more source