Results 31 to 40 of about 15,941 (260)

Loop optimization of Trichoderma reesei endoglucanases for balancing the activity–stability trade‐off through cross‐strategy between machine learning and the B‐factor analysis

GCB Bioenergy, Volume 15, Issue 2, Page 128-142, February 2023., 2023
A cross‐strategy combination of machine learning and B‐factor analysis was used to predict beneficial amino acid substitution in endoglucanase (EG) loop optimization. Experimental validation showed single‐site mutated EG concomitantly improved enzymatic activity and thermal properties by 17.21%–18.06% and 49.85%–62.90%, respectively, compared with wild‐
Le Gao, Qi Guo, Ruinan Xu, Haofan Dong, Chichun Zhou, Zhuohang Yu, Zhaokun Zhang, Lixian Wang, Xiaoyi Chen, Xin Wu   +9 more
wiley   +1 more source

Induction and Characterisation of Lignocellulolytic Activities from Novel Deep-Sea Fungal Secretomes

Fermentation, 2023
Fungi are increasingly recognised as being able to inhabit extreme environments. The deep sea is considered an extreme environment because of its low temperatures, high hydrostatic and lithostatic pressures, 3.5% salinity, and low oxygen, nutrient and ...
Bronwyn Dowd, Maria G. Tuohy
doaj   +1 more source

How a Glycoside Hydrolase Recognizes a Helical Polyglucan [PDF]

Structure, 2017
Similarly to other biopolymers, linear polysaccharides can form double- or triple-helical structures. How enzymes recognize and manage this quaternary structure is an unresolved question. In this issue of Structure, Pluvinage et al. (2017) shed light on the structural complementarity between family GH81 glycoside hydrolase and the quaternary structure ...
Bernard Henrissat, Bernard Henrissat, Bernard Henrissat, Marie-Line Garron, Marie-Line Garron   +4 more
openaire   +6 more sources

Enzyme Similarity Networks [PDF]

arXiv, 2022
There is a crescent use of enzymes in multiple industries and sciences, ranging from materials and fuel synthesis to pharmaceutical and food production. Their applicability in this variety of fields depends not only on their biochemical function but also on their physicochemical properties.
arxiv  

Link between allosteric signal transduction and functional dynamics in a multi-subunit enzyme: S-adenosylhomocysteine hydrolase [PDF]

J. Am. Chem. Soc. (2011) vol. 133, 19807-19815, 2011
S-adenosylhomocysteine hydrolase (SAHH), a cellular enzyme that plays a key role in methylation reactions including those required for maturation of viral mRNA, is an important drug target in the discovery of antiviral agents. While targeting the active site is a straightforward strategy of enzyme inhibition, evidences of allosteric modulation of ...
arxiv   +1 more source

Curation of characterized glycoside hydrolases of Fungal origin [PDF]

Database, 2011
Fungi produce a wide range of extracellular enzymes to break down plant cell walls, which are composed mainly of cellulose, lignin and hemicellulose. Among them are the glycoside hydrolases (GH), the largest and most diverse family of enzymes active on these substrates.
Murphy, Caitlin, Powlowski, Justin, Wu, Min, Butler, Greg, Tsang, Adrian   +4 more
openaire   +4 more sources

Interlinked GTPase cascades provide a motif for both robust switches and oscillators [PDF]

J. R. Soc. Interface 16, 20190198 (2019), 2019
GTPases regulate a wide range of cellular processes, such as intracellular vesicular transport, signal transduction, and protein translation. These hydrolase enzymes operate as biochemical switches by toggling between an active guanosine triphosphate (GTP)-bound state and an inactive guanosine diphosphate (GDP)-bound state.
arxiv   +1 more source

Rewiring of the seed metabolome during Tartary buckwheat domestication

Plant Biotechnology Journal, Volume 21, Issue 1, Page 150-164, January 2023., 2023
Summary Crop domestication usually leads to the narrowing genetic diversity. However, human selection mainly focuses on visible traits, such as yield and plant morphology, with most metabolic changes being invisible to the naked eye. Buckwheat accumulates abundant bioactive substances, making it a dual‐purpose crop with excellent nutritional and ...
Hui Zhao, Yuqi He, Kaixuan Zhang, Shijuan Li, Yong Chen, Ming He, Feng He, Bin Gao, Di Yang, Yu Fan, Xuemei Zhu, Mingli Yan, Nathalie Giglioli‐Guivarc'h, Christophe Hano, Alisdair R. Fernie, Milen I. Georgiev, Dagmar Janovská, Vladimir Meglič, Meiliang Zhou   +18 more
wiley   +1 more source

The XTH Gene Family in Schima superba: Genome-Wide Identification, Expression Profiles, and Functional Interaction Network Analysis

Frontiers in Plant Science, 2022
Xyloglucan endotransglucosylase/hydrolase (XTH), belonging to glycoside hydrolase family 16, is one of the key enzymes in plant cell wall remodeling. Schima superba is an important timber and fireproof tree species in southern China.
Zhongyi Yang, Zhongyi Yang, Rui Zhang, Rui Zhang, Zhichun Zhou, Zhichun Zhou   +5 more
doaj   +1 more source

Aberrant Autophagy Impacts Growth and Multicellular Development in a Dictyostelium Knockout Model of CLN5 Disease

Frontiers in Cell and Developmental Biology, 2021
Mutations in CLN5 cause a subtype of neuronal ceroid lipofuscinosis (NCL) called CLN5 disease. While the precise role of CLN5 in NCL pathogenesis is not known, recent work revealed that the protein has glycoside hydrolase activity.
Meagan D. McLaren, Sabateeshan Mathavarajah, William D. Kim, Shyong Q. Yap, Robert J. Huber   +4 more
doaj   +1 more source