Results 31 to 40 of about 27,041 (188)

The evolutionary advantage of an aromatic clamp in plant family 3 glycoside exo-hydrolases

Nature Communications, 2022
Barley β-d-glucan glucohydrolase is a glycoside hydrolase family 3 (GH3) enzyme critical for growth and development. Here the authors carryout mutagenesis, structural analyses and multi-scale molecular dynamics to examine the binding and conformational ...
Sukanya Luang, Xavier Fernández-Luengo, Alba Nin-Hill, Victor A. Streltsov, Julian G. Schwerdt, Santiago Alonso-Gil, James R. Ketudat Cairns, Stéphanie Pradeau, Sébastien Fort, Jean-Didier Maréchal, Laura Masgrau, Carme Rovira, Maria Hrmova   +12 more
doaj   +1 more source

Structural changes and molecular mechanism study on the inhibitory activity of epigallocatechin against α-glucosidase and α-amylase

Frontiers in Nutrition, 2022
In this study, the inhibition and mechanism of epigallocatechin (EGC) on two key glycoside hydrolases (α-glucosidase, α-amylase) were explored from the molecular structure level.
Ziyi Man, Yi Feng, Jibo Xiao, Hailong Yang, Xiangting Wu   +4 more
doaj   +1 more source

The mucin-degradation strategy of Ruminococcus gnavus:The importance of intramolecular trans-sialidases [PDF]

, 2016
We previously identified and characterized an intramolecular trans-sialidase (IT-sialidase) in the gut symbiont Ruminococcus gnavus ATCC 29149, which is associated to the ability of the strain to grow on mucins. In this work we have obtained and analyzed
Crossman, Lisa C., Crost, Emmanuelle H, Henrissat, Bernard, Juge, Nathalie, Monestier, Marie, Swarbreck, David, Tailford, Louise E   +6 more
core   +2 more sources

Structure and activity of the Streptococcus pyogenes family GH1 6-phospho β-glycosidase, Spy1599 [PDF]

, 2013
The group A streptococcus Streptococcus pyogenes is the causative agent of a wide spectrum of invasive infections, including necrotizing fasciitis, scarlet fever and toxic shock syndrome.
Aguilar-Moncayo, Ajdić, Cantarel, Collin, Conejo, Cowtan, Cowtan, Edward J. Taylor, Emsley, Evans, Fujita, Gideon J. Davies, Gregg, Harry Brumer, Henrissat, Henrissat, Hondorp, Jens M. Eklof, Jerome Dabin, Jin, Johan P. Turkenburg, Judith Stepper, Kabsch, Kietzman, Kori, Leslie, McNicholas, Murshudov, Padilla, Pancholi, Prachumporn Kongsaeree, Preeyanuch Thongpoo, Ryan, Ryan, Seshadri, Shelburne, Shelburne, Shelburne, Sheldon, Suck, Suits, Vagin, Wiesmann, Wiesmann, Winn, Winter, Wright, Zechel   +47 more
core   +1 more source

Transcriptome Profiling-Based Analysis of Carbohydrate-Active Enzymes in Aspergillus terreus Involved in Plant Biomass Degradation

Frontiers in Bioengineering and Biotechnology, 2020
Given the global abundance of plant biomass residues, potential exists in biorefinery-based applications with lignocellulolytic fungi. Frequently isolated from agricultural cellulosic materials, Aspergillus terreus is a fungus efficient in secretion of ...
Camila L. Corrêa, Glaucia E. O. Midorikawa, Edivaldo Ximenes Ferreira Filho, Eliane Ferreira Noronha, Gabriel S. C. Alves, Roberto Coiti Togawa, Orzenil Bonfim Silva-Junior, Marcos Mota do Carmo Costa, Priscila Grynberg, Robert N. G. Miller   +9 more
doaj   +1 more source

Structure and stereochemistry of the base excision repair glycosylase MutY reveal a mechanism similar to retaining glycosidases. [PDF]

, 2015
MutY adenine glycosylases prevent DNA mutations by excising adenine from promutagenic 8-oxo-7,8-dihydroguanine (OG):A mismatches. Here, we describe structural features of the MutY active site bound to an azaribose transition state analog which indicate a
Cao, Sheng, Chu, Aurea, David, Sheila S, Horvath, Martin P, O'Shea, Valerie L, Richards, Jody L, Woods, Ryan D   +6 more
core   +2 more sources

Development and validation of a microarray for the investigation of the CAZymes encoded by the human gut microbiome. [PDF]

PLoS ONE, 2013
Distal gut bacteria play a pivotal role in the digestion of dietary polysaccharides by producing a large number of carbohydrate-active enzymes (CAZymes) that the host otherwise does not produce.
Abdessamad El Kaoutari, Fabrice Armougom, Quentin Leroy, Bernard Vialettes, Matthieu Million, Didier Raoult, Bernard Henrissat   +6 more
doaj   +1 more source

Ruminococcal cellulosome systems from rumen to human [PDF]

, 2015
This article is protected by copyright. All rights reserved. The authors appreciate the kind assistance of Miriam Lerner (ImmunArray Ltd. Company, Rehovot, Israel) with experiments involving the MicroGrid II arrayer.
Bayer, Edward A, Bernalier-Donadille, Annick, Borovok, Ilya, Dassa, Bareket, David, Yonit Ben, Duncan, Sylvia H, Flint, Harry J, Koropatkin, Nicole M, Lamed, Raphael, Martens, Eric C, Moraïs, Sarah, White, Bryan A   +11 more
core   +3 more sources

Identification of d-arabinan-degrading enzymes in mycobacteria

Nature Communications, 2023
Bacterial cell growth and division require the coordinated action of enzymes that synthesize and degrade cell wall polymers. Here, we identify enzymes that cleave the d-arabinan core of arabinogalactan, an unusual component of the cell wall of ...
Omar Al-Jourani, Samuel T. Benedict, Jennifer Ross, Abigail J. Layton, Phillip van der Peet, Victoria M. Marando, Nicholas P. Bailey, Tiaan Heunis, Joseph Manion, Francesca Mensitieri, Aaron Franklin, Javier Abellon-Ruiz, Sophia L. Oram, Lauren Parsons, Alan Cartmell, Gareth S. A. Wright, Arnaud Baslé, Matthias Trost, Bernard Henrissat, Jose Munoz-Munoz, Robert P. Hirt, Laura L. Kiessling, Andrew L. Lovering, Spencer J. Williams, Elisabeth C. Lowe, Patrick J. Moynihan   +25 more
doaj   +1 more source

Identification of Carbohydrate Metabolism Genes in the Metagenome of a Marine Biofilm Community Shown to Be Dominated by Gammaproteobacteria and Bacteroidetes [PDF]

, 2010
Polysaccharides are an important source of organic carbon in the marine environment and degradation of the insoluble and globally abundant cellulose is a major component of the marine carbon cycle.
Connolly, John, Cox, Michael, Edwards, Clive, Edwards, Jennifer, Joint, Ian, McCarthy, Alan, McDonald, James, Smith, Darren   +7 more
core   +2 more sources