Results 31 to 40 of about 1,222 (163)

Investigating the Role of Antigen Orientation on the Immune Response Elicited by Neisseria meningitidis Factor H Binding Protein on GMMA

Vaccines, 2022
GMMA are outer membrane vesicles (OMVs) released from Gram-negative bacteria genetically modified to enhance OMVs formation that have been shown to be optimal systems to enhance immunogenicity of protein antigens. Here, we selected Neisseria meningitidis
Renzo Alfini, Brunella Brunelli, Erika Bartolini, Martina Carducci, Enrico Luzzi, Francesca Ferlicca, Scilla Buccato, Barbara Galli, Paola Lo Surdo, Maria Scarselli, Giacomo Romagnoli, Elena Cartocci, Domenico Maione, Silvana Savino, Francesca Necchi, Isabel Delany, Francesca Micoli   +16 more
doaj   +3 more sources

Quality by Design Framework Applied to GMMA Purification [PDF]

The AAPS Journal
In recent years, Generalized Modules for Membrane Antigens (GMMA) have received increased attention as an innovative vaccine platform against bacterial pathogens, particularly attractive for low- and middle-income countries because of manufacturing ...
C. Giannelli, Francesca Necchi, Elena Palmieri, D. Oldrini, Beatrice Ricchetti, Maria M. Papathanasiou, Zoltán Kis, C. Kontoravdi, C. Campa, F. Micoli   +9 more
semanticscholar   +5 more sources

GMMA and Glycoconjugate Approaches Compared in Mice for the Development of a Vaccine against Shigella flexneri Serotype 6 [PDF]

Vaccines, 2020
Shigella infections are one of the top causes of diarrhea throughout the world, with Shigella flexneri being predominant in developing countries. Currently, no vaccines are widely available and increasing levels of multidrug-resistance make Shigella a ...
Maria Michelina Raso, Gianmarco Gasperini, Renzo Alfini, Fabiola Schiavo, Maria Grazia Aruta, Martina Carducci, Maria Concetta Forgione, Silvia Martini, Paola Cescutti, Francesca Necchi, Francesca Micoli   +10 more
doaj   +6 more sources

Antibodies elicited by altSonflex1-2-3 GMMA vaccine are bactericidal against a panel of drug-resistant <i>Shigella</i> clinical isolates. [PDF]

Front Immunol
Shigellosis significantly impacts global health, particularly affecting vulnerable populations in low- and middle-income countries, with over 270 million annual infections.
Boero E, Di Benedetto R, Vezzani G, Alfini R, Ceyssens PJ, Tansarli GS, Fang FC, Fontana C, Rossi A, Carrara S, Mancini F, Iturriza M, Sala C, Giannelli C, Rossi O, Micoli F.   +15 more
europepmc   +2 more sources

Neisseria meningitidis Factor H Binding Protein Surface Exposure on Salmonella Typhimurium GMMA Is Critical to Induce an Effective Immune Response against Both Diseases [PDF]

Pathogens, 2021
GMMA, outer membrane vesicles resulting from hyperblebbing mutated bacterial strains, are a versatile vaccine platform for displaying both homologous and heterologous antigens.
Francesca Necchi, Giuseppe Stefanetti, Renzo Alfini, Elena Palmieri, Martina Carducci, Roberta Di Benedetto, Fabiola Schiavo, Maria Grazia Aruta, Fabiola Giusti, Ilaria Ferlenghi, Yun Shan Goh, Simona Rondini, Francesca Micoli   +12 more
doaj   +2 more sources

Antigen Presentation by Follicular Dendritic Cells to Cognate B Cells Is Pivotal for Generalised Modules for Membrane Antigens (GMMA) Immunogenicity

Vaccine, 2022
GMMA has been proposed as a potent technology platform for the design of safe, effective and affordable vaccines. As GMMA are vesicles blebbing out of the outer membrane of Gram-negative bacteria, they contain lipopolysaccharides, lipoproteins and ...
Diego Piccioli, R. Alfini, V. Monaci, V. Arato, M. Carducci, M. G. Aruta, O. Rossi, Francesca Necchi, A. Anemona, E. Bartolini, F. Micoli   +10 more
exaly   +2 more sources

Production of a Shigella sonnei Vaccine Based on Generalized Modules for Membrane Antigens (GMMA), 1790GAHB. [PDF]

PLoS ONE, 2015
Recently, we developed a high yield production process for outer membrane particles from genetically modified bacteria, called Generalized Modules of Membrane Antigens (GMMA), and the corresponding simple two step filtration purification, enabling ...
Christiane Gerke, Anna Maria Colucci, Carlo Giannelli, Silvia Sanzone, Claudia Giorgina Vitali, Luigi Sollai, Omar Rossi, Laura B Martin, Jochen Auerbach, Vito Di Cioccio, Allan Saul   +10 more
doaj   +4 more sources

Characterization of Competitive ELISA and Formulated Alhydrogel Competitive ELISA (FAcE) for Direct Quantification of Active Ingredients in GMMA-Based Vaccines

Methods and Protocols, 2020
Generalized modules for membrane antigens (GMMA) represent a technology particularly attractive for designing affordable vaccines against Gram-negative bacteria. We explored such technology for the development of O-antigen-based vaccines against Shigella
Omar Rossi, Maria Grazia Aruta, Alessandra Acquaviva, Francesca Mancini, Francesca Micoli, Francesca Necchi   +5 more
doaj   +2 more sources

Development of a Monocyte Activation Test as an Alternative to the Rabbit Pyrogen Test for Mono- and Multi-Component Shigella GMMA-Based Vaccines

Microorganisms, 2021
Generalised modules for membrane antigens (GMMA)-based vaccines comprise the outer membrane from genetically modified Gram-negative bacteria containing membrane proteins, phospholipids and lipopolysaccharides.
Danielle Carson, Sophie Myhill, Elena Palmieri, Francesca Necchi, Sjoerd Rijpkema, Francesca Micoli, Ida Karin Nordgren, Omar Rossi, Caroline Vipond   +8 more
doaj   +2 more sources

Preclinical evaluation of multivalent vaccine combinations against Shigella and Salmonella infections [PDF]

Frontiers in Immunology
Shigellosis is an intestinal infection causing severe and often life-threatening diarrheal disease, with high prevalence in children under five years of age in low- and middle-income countries.
Roberta Di Benedetto, Roberta Di Benedetto, Rebecca Nappini, Salvatore Gemmellaro, Federica Boretto, Valentina Caradonna, Martina Carducci, Paola Cescutti, Omar Rossi, Francesca Mancini, Francesco Berlanda Scorza, Simona Rondini, Carlo Giannelli, Renzo Alfini, Francesca Micoli   +14 more
doaj   +2 more sources