Results 161 to 170 of about 117,081 (314)

What Makes an “Ideal” Cell Line for Recombinant Adeno‐Associated Virus Production?

Biotechnology and Bioengineering, EarlyView.
Several host cell types have been used to produce rAAVs to date. Cell line‐specific traits that are beneficial in the context of rAAV manufacturing are reviewed here, with the goal of developing a consensus on the ‘ideal’ characteristics that an rAAV production host should possess (created with Biorender.com).
James Conheady, Nicholas Donohue, Alexandra Bogdanovic, Sharon Davin, Brian Glennon, Niall Barron, C. Mark Smales   +6 more
wiley   +1 more source

Cyclic di-GMP directly reprograms the multidrug resistance machinery via UspG-mediated sequestration of RamR in Klebsiella. [PDF]

PLoS Pathog
Liu X, Wang M, Fu Y, Zhu X, Du M, Wen Y, Liao Y, Zhao Y, Deng Y, Gu B.   +9 more
europepmc   +1 more source

Cell and Gene Therapies Manufacturing Challenges and Integrated Good Manufacturing Practices Solutions: A Lifecycle Perspective

Biotechnology and Bioengineering, EarlyView.
Lifecycle perspective on cell and gene therapy manufacturing challenges and enabling GMP solutions. ABSTRACT Cell and gene therapies (CGTs) are revolutionizing modern medicine; however, making these advanced medicines scalable and readily available to commercial manufacturers worldwide is a major challenge. The number of approved CGT products continues
Rajath Samaga, Udisha Singh, Sachin Dattram Pawar   +2 more
wiley   +1 more source

Sähkö- ja automaatiotöiden muutokset GMP-ympäristössä [PDF]

Opinnäytetyössä tutkittiin sähkö- ja automaatiojärjestelmien muutostöiden vaatimuksia ja toteutusta lääketeollisuudessa, GMP-ympäristössä. Työn tavoitteena oli selvittää sähkö- ja automaatiojärjestelmien muutostöiden kvalifiointiprosessin vaiheet ja ...
Suojanen, Joni
core  

Process Analytical Technology (PAT) for Continuous Purification of mRNA via Precipitation and Tangential Flow Filtration

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Continuous downstream processing of messenger ribonucleic acid (mRNA) therapeutics requires purification strategies that are scalable, robust, and capable of removing product‐ and process‐related impurities from in vitro transcription mixtures.
Rajan R. Bhawnani, Maria del Carme Pons Royo, Rohan Kadambi, Andrew Hatas, Steve Burcat, Tyler Arnold, Hasan Al‐Mahayni, Richard D. Braatz, Allan S. Myerson   +8 more
wiley   +1 more source

Conjugation of Coffee Phenolics With Milk Bioactive Peptides: Characterization of Coffee and Apple Phenolics and Evaluation of Polyphenol Oxidase as a Natural Biocatalyst. [PDF]

Food Sci Nutr
Hamadneh BN, Mustafa ED, Alkafafy ME, Khojah E, Samra BN, Wapet DEM, Mahmoud MM, Badawy WZ, Khalil M, Salama MA, Abdin M, Ali M.   +11 more
europepmc   +1 more source

The structral aspects of cyclic GMP and cyclic di-GMP-dependent bacterial signaling pathways

, 2015
二級訊息傳導物質Cyclic guanosine 3',5'-monophosphate (cyclic GMP) 在細菌訊號傳遞中所扮演的功能在過去並非相當明確。然而在植物病原菌Xanthomonas campestris pv campestris (Xcc)的基因篩選實驗中發現，編碼XC_0250會轉譯出第三型nucleotidyl 環化酶，並合成cyclic GMP。在in vitro的實驗也證實純化出來的XC_0250具有合成cyclic GMP的能力。而其相連的基因XC_0249 ...
楊詔貴, Jauo-Guey Yang
core  

Identification of Putative Cellulose Synthase Gene xcsA2 Required for c‐di‐GMP‐Induced Biofilm Formation and Cell Aggregation in Synechococcus elongatus PCC 7942

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Synechococcus elongatus PCC 7942 is a widely used model cyanobacterium for diverse research fields and for bioproduction applications owing to its ease of cultivation, genetic modification, and ability to fix CO2. We found that a laboratory strain of S. elongatus PCC 7942 expressing the ydeH gene from E.
Chihiro C. Yamaguchi, Jiro F. Mori, Shinsuke Kutsuna, Robert A. Kanaly   +3 more
wiley   +1 more source

Co-Expression of PA0290 and PelD Enhances <i>Pseudomonas aeruginosa</i> Biofilm Formation. [PDF]

Microorganisms
Zhan X, Pei Y, Huang Y, Li N, Huang W, Tang Y, Liang H.   +6 more
europepmc   +1 more source

Efficient Cell Removal and Impurity Reduction for Poxvirus Purification in a Single Step Alluvial Filtration

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Modified Vaccinia virus Ankara (MVA) is a potent vector for the development of vaccines and novel cancer treatment strategies. Since it is a non‐replicating virus in human recipients, high infective titers are required for therapeutic efficacy.
Linus G. Weber, Caroline Stephan, Georgios Makris, Leander John, Ingo Jordan, Michael W. Wolff   +5 more
wiley   +1 more source