Expression in bacteria of the gene encoding the gp43 antigen of Paracoccidioides brasiliensis: Immunological reactivity of the recombinant fusion proteins [PDF]
, 2002 gp43 is the major diagnostic antigen of Paracoccidioides brasiliensis, the agent of paracoccidioidomycosis IPCM) in humans. in the present study, cDNA of the gp43 gene (PbGP43) was obtained by reverse transcriptase PCR, inserted into a pGEX vector in ...
Cisalpino, Patricia S. +5 more
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Paracoccidioides brasiliensis-gp43 used as paracoccidioidin
, 2015 A purified glycoprotein of 43 000 daltons from Paracoccidioides brasiliensis (gp43) was tested as paracoccidiodin in delayed-type hypersensitivity (DTH) tests in both experimental animals (guinea pig and mice) and patients with paracoccidioidomycosis ...
Unterkircher, CS +4 more
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Natural history of experimental arthritis induced by Paracoccidioides brasiliensis in wistar rats
Revista da Sociedade Brasileira de Medicina Tropical, 2019 INTRODUCTION: Paracoccidioidomycosis (PCM) is the most prevalent systemic mycosis in Latin America. This study aimed to evaluate the natural history of Paracoccidioides brasiliensis-induced experimental arthritis of the knee joints in Wistar rats ...
Eduardo Alexandre Loth +5 more
doaj +1 more source
RNA interference of Pb18 GP43 gene expression triggered artificially with intron-containing dsRNA.
, 2014 (A). GP43 gene expression levels were investigated in six cAlf-GP43 transformants in relation to cAlfG-control by qRT-PCR after 8 months of subculturing in non-selective media. Results were normalized against the internal control L34.
Cristiane R. Correa (638501) +7 more
core +1 more source
Recombinant vaccines of a CD4+ T-cell epitope promote efficient control of Paracoccidioides brasiliensis burden by restraining primary organ infection. [PDF]
PLoS Neglected Tropical Diseases, 2017 Paracoccidioidomycosis (PCM) is an infectious disease endemic to South America, caused by the thermally dimorphic fungi Paracoccidioides. Currently, there is no effective human vaccine that can be used in prophylactic or therapeutic regimes.
Rodrigo Assunção Holanda +10 more
doaj +1 more source
Clinical features and genetic background of the sympatric species Paracoccidioides brasiliensis and Paracoccidioides americana. [PDF]
PLoS Neglected Tropical Diseases, 2019 INTRODUCTION:The agents of paracoccidioidomycosis, historically identified as Paracoccidioides brasiliensis, are in fact different phylogenetic species.
Priscila Marques de Macedo +5 more
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Use of recombinant gp43 isoforms expressed in Pichia pastoris for diagnosis of paracoccidioidomycosis [PDF]
, 2008 gp43 is the main diagnostic antigen for paracoccidioidomycosis (PCM). in vitro, gp43 expression in supernatant fluids of Paracoccidioides brasiliensis cultures can be unstable, and its regulation is poorly understood. We have been able to express soluble
Vallejo, M. C. [UNIFESP] +3 more
core +1 more source
DNA polymorphisms in different groups to loci GP43 + ARF in 174 Paracoccidioides spp. isolates.
, 2021 DNA polymorphisms in different groups to loci GP43 + ARF in 174 Paracoccidioides spp. isolates.
Marcus de Melo Teixeira (161154) +9 more
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Semina: Ciências Agrárias, 2009 Paracoccidioidomycosis, caused by the dimorphic fungus Paracoccidioides brasiliensis, is the most prevalent human systemic mycosis in Latin America. Paracoccidioidomycosis affects mainly male rural workers, causing granulomatous lesions in organs such as
Mario Augusto Ono +4 more
doaj +1 more source
Expression and isoforms of gp43 in different strains of Paracoccidioides brasiliensis
, 1995 Individual exoantigens from 13 Paracoccidioides brasiliensis isolates were obtained and tested against a panel of 50 sera from patients with paracoccidioidomycosis by immunodiffusion test.
Lopes, Jose Daniel [UNIFESP] +4 more
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