Results 171 to 180 of about 56,362 (206)
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Lipid Rafts, Caveolae and GPI-Linked Proteins
2012Lipid rafts and caveolae are specialized membrane microdomains enriched in sphingolipids and cholesterol. They function in a variety of cellular processes including but not limited to endocytosis, transcytosis, signal transduction and receptor recycling.
Reeves, V.L. +2 more
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Biochimica et Biophysica Acta (BBA) - General Subjects, 1993
A number of enzymes are known to release alkaline phosphatase and other glycan phosphatidylinositol-anchored proteins from membrane surfaces. We describe a novel approach to detect and measure these activities by partitioning in aqueous phase systems. The procedures avoid the complications of micelle-formation involving hydrophobic molecules that may ...
F D, Raymond, D W, Moss, D, Fisher
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A number of enzymes are known to release alkaline phosphatase and other glycan phosphatidylinositol-anchored proteins from membrane surfaces. We describe a novel approach to detect and measure these activities by partitioning in aqueous phase systems. The procedures avoid the complications of micelle-formation involving hydrophobic molecules that may ...
F D, Raymond, D W, Moss, D, Fisher
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Biochimica et Biophysica Acta (BBA) - General Subjects, 1997
GPI-anchored proteins are distributed ubiquitously in eukaryotes, but not in procaryotes. By metabolic-labeling of Sulfolobus acidocaldarius cells, 14C-radiolabeled precursors of GPI and caldarchaetidylinositol were incorporated into 120, 143 and 185 kDa proteins.
T, Kobayashi, R, Nishizaki, H, Ikezawa
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GPI-anchored proteins are distributed ubiquitously in eukaryotes, but not in procaryotes. By metabolic-labeling of Sulfolobus acidocaldarius cells, 14C-radiolabeled precursors of GPI and caldarchaetidylinositol were incorporated into 120, 143 and 185 kDa proteins.
T, Kobayashi, R, Nishizaki, H, Ikezawa
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Biochemistry, 2000
Exposure of cells to liposomes results in the release of integral membrane proteins. However, it is still controversial whether the release is due to spontaneous protein transfer from cells to liposomes or shed vesicles released from cells. We investigated this issue in an erythrocyte-liposome system by examining the location of acetylcholinesterase ...
K, Suzuki, Y, Okumura
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Exposure of cells to liposomes results in the release of integral membrane proteins. However, it is still controversial whether the release is due to spontaneous protein transfer from cells to liposomes or shed vesicles released from cells. We investigated this issue in an erythrocyte-liposome system by examining the location of acetylcholinesterase ...
K, Suzuki, Y, Okumura
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British Journal of Haematology, 1992
Summary.We have studied three glycosylphosphatidylinositol (GPI) linked proteins on the erythrocytes of 14 patients with paroxysmal nocturnal haemoglobinuria (PNH). The pattern observed was bimodal in 12 of the patients and trimodal in two. Ten patients had a red cell population with normal CD59 antigen (membrane inhibitor of reactive lysis, MIRL ...
P, Hillmen, J M, Hows, L, Luzzatto
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Summary.We have studied three glycosylphosphatidylinositol (GPI) linked proteins on the erythrocytes of 14 patients with paroxysmal nocturnal haemoglobinuria (PNH). The pattern observed was bimodal in 12 of the patients and trimodal in two. Ten patients had a red cell population with normal CD59 antigen (membrane inhibitor of reactive lysis, MIRL ...
P, Hillmen, J M, Hows, L, Luzzatto
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Molecular Reproduction and Development, 2008
AbstractSperm uptake of glycosyl phosphatidylinositol (GPI)‐linked proteins from luminal fluids has been shown to occur in male and estrous female reproductive tracts. In males, this is attributed to membranous vesicles secreted into the epididymis and prostate. While epididymosomes have been characterized, there have been no reports of the presence of
Genevieve S, Griffiths +3 more
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AbstractSperm uptake of glycosyl phosphatidylinositol (GPI)‐linked proteins from luminal fluids has been shown to occur in male and estrous female reproductive tracts. In males, this is attributed to membranous vesicles secreted into the epididymis and prostate. While epididymosomes have been characterized, there have been no reports of the presence of
Genevieve S, Griffiths +3 more
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British Journal of Haematology, 1994
SummaryParoxysmal nocturnal haemoglobinuria (PNH) is due to the absence or marked reduction of glycan phosphatidylinositol (GPI)‐anchored proteins on the surface of blood cells. Affected patients may have a population of red blood cells that are completely deficient (PNH III) or partially deficient (PNH II) in these proteins, or they may have both. PNH
M, Bessler +3 more
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SummaryParoxysmal nocturnal haemoglobinuria (PNH) is due to the absence or marked reduction of glycan phosphatidylinositol (GPI)‐anchored proteins on the surface of blood cells. Affected patients may have a population of red blood cells that are completely deficient (PNH III) or partially deficient (PNH II) in these proteins, or they may have both. PNH
M, Bessler +3 more
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Protein Expression and Purification, 2004
Melanotransferrin, also named p97, belongs to the transferrin-like group of iron-binding proteins. Unlike the other members of this family, p97 exists in two forms-one soluble form and one attached to the cell membrane by a glycosylphosphatidylinositol (GPI) anchor.
Joseph, Yang +3 more
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Melanotransferrin, also named p97, belongs to the transferrin-like group of iron-binding proteins. Unlike the other members of this family, p97 exists in two forms-one soluble form and one attached to the cell membrane by a glycosylphosphatidylinositol (GPI) anchor.
Joseph, Yang +3 more
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2003
Clinical characteristics of PNH include anemia due to intravascular hemolysis and hemoglobinuria, complications caused by bone marrow failure, and recurrent thrombosis. Despite the cloning of the Piga gene and the recent insights into the molecular lesion that causes the loss of glycosyl phosphatidylinositol-(GPI) linked proteins, much of the ...
Monica Bessler +5 more
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Clinical characteristics of PNH include anemia due to intravascular hemolysis and hemoglobinuria, complications caused by bone marrow failure, and recurrent thrombosis. Despite the cloning of the Piga gene and the recent insights into the molecular lesion that causes the loss of glycosyl phosphatidylinositol-(GPI) linked proteins, much of the ...
Monica Bessler +5 more
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Expression of GPI-linked Green Fluorescent Protein on the surface of CHO cells
Biochemical Society Transactions, 2000S. Hiscox +2 more
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