Results 161 to 170 of about 1,577 (195)

Antigen epitope screening of grass carp reovirus and its protectively immunity assessment for grass carp

Aquaculture, 2020
Abstract Hemorrhagic disease caused by grass carp reovirus (GCRV) is considered to be one of the most serious threats to grass carp, resulting in significant economic loss in aquaculture industry. Currently, vaccination is the most effective method to against GCRV infection and improve the production of grass carp.
Zi-Rao Guo, Yi-Jun Jia, Gao-Xue Wang
exaly   +2 more sources

Characterization of the nonstructural protein NS80 of grass carp reovirus

Archives of Virology, 2010
Nonstructural proteins of members of the family Reoviridae are believed to play significant roles in the virus replication cycle. Phylogenetic analyses indicate that the nonstructural protein NS80 of grass carp reovirus, encoded by a gene of Segment 4 (S4), is a primary determinant that is related to the formation of viroplasmic inclusion bodies (VIB),
Chao, Fan, Ling, Shao, Qin, Fang
openaire   +2 more sources

Detection of grass carp reovirus (GCRV) with monoclonal antibodies

Archives of Virology, 2013
Grass carp reovirus (GCRV) is a pathogen that causes hemorrhagic disease of grass carp. It is the most serious infectious disease of carp and causes serious losses of fingerlings of grass carp and black carp. In this study, a recombinant VP4, one of the viral core proteins, was constructed with a histidine tag and expressed at a high level in E.
Jing, Hongli, Zhang, Lifeng, Fang, Zhenzhen, Xu, Lipu, Zhang, Min, Wang, Na, Jiang, Yulin, Lin, Xiangmei   +7 more
openaire   +2 more sources

Characterization of grass carp CD40 and CD154 genes and the association between their polymorphisms and resistance to grass carp reovirus

Fish & Shellfish Immunology, 2018
In bony fish, CD40 and CD154 are two very important costimulatory molecules involved in T and B cell cooperation in thymus-dependent antibody production. In the current study, we identified the cDNAs of CD40 and CD154 and analyzed their genomic structures in grass carp.
Xiao-Bing Lu, Ya-Xin Chen, Zheng-Wei Cui, Xiang-Yang Zhang, Long-Feng Lu, Shun Li, Xiao-Qin Xia, Pin Nie, Yong-An Zhang   +8 more
openaire   +2 more sources

Non-pathogenic grass carp reovirus infection leads to both apoptosis and autophagy in a grass carp cell line

Fish & Shellfish Immunology, 2022
A novel GCRV strain isolated from healthy grass carp was named as grass carp reovirus - HH196 (GCRV-HH196), and its infection mechanism remains unclear. In this study, the grass carp ovary cell line (GCO cells) was used to investigate the cell death involved in GCRV-HH196 infection.
Yanlin Dai, Yiqun Li, Ge Lin, Jingjing Zhang, Nan Jiang, Wenzhi Liu, Yan Meng, Yong Zhou, Yuding Fan   +8 more
openaire   +2 more sources

Genomic structure of grass carp Mx2 and the association of its polymorphisms with susceptibility/resistance to grass carp reovirus

Molecular Immunology, 2011
Mx (myxovirus-resistant) proteins are induced by interferon and inhibit viral replication in many vertebrates. In the present study, the organization of grass carp (Ctenopharyngodon idella) Mx2 (CiMx2) gene sequence was elucidated and its single nucleotide polymorphisms (SNPs) were investigated to explore their association with susceptibility ...
Lan, Wang, Jianguo, Su, Limin, Peng, Jianfu, Heng, Lijun, Chen   +4 more
openaire   +2 more sources

Characterization of infectious particles of grass carp reovirus by treatment with proteases

Archives of Virology, 2008
Proteolytic cleavages play an important role in reovirus infection during entry into cells. The effects of protease digestion on the morphology, infectivity and polypeptide composition of grass carp reovirus (GCRV) were investigated. Following treatment with chymotrypsin, the different subviral particles of GCRV were isolated using density gradient ...
Q, Fang, E K, Seng, Q Q, Ding, L L, Zhang   +3 more
openaire   +2 more sources

Serodiagnosis of grass carp reovirus infection in grass carp Ctenopharyngodon idella by a novel Western blot technique

Journal of Virological Methods, 2013
Frequent outbreaks of grass carp hemorrhagic disease, caused by grass carp reovirus (GCRV) infection, pose as serious threats to the production of grass carp Ctenopharyngodon idella. Although various nucleic acids-based diagnostic methods have been shown effective, lack of commercial monoclonal antibody against grass carp IgM has impeded the ...
Yongxing, He, Yousheng, Jiang, Liqun, Lu
openaire   +2 more sources

Identification of a potential transcriptional regulator encoded by grass carp reovirus

Archives of Virology, 2019
Many transcription factors are encoded by DNA viruses and retroviruses due to their regulatory roles in gene expression in the host cell. However, no transcriptional regulator has been identified in any reovirus. Here, a non-structural protein, NS31, encoded by grass carp reovirus genomic segment S7 was characterized.
Fei, Yu, Hao, Wang, Longlong, Wang, Wanjuan, Li, Liqun, Lu   +4 more
openaire   +2 more sources

3D reconstruction and capsid protein characterization of grass carp reovirus

Science in China Series C, 2005
Grass carp reovirus (GCRV) is a relatively new virus first isolated in China and is a member of the Aquareovirus genus of the Reoviridae family. Recent report of genomic sequencing showed that GCRV shared high degree of homology with mammalian reovirus (MRV). As a step of our effort to understand the structural basis of GCRV pathogenesis, we determined
Qin, Fang, Sanket, Shah, Yuyao, Liang, Z H, Zhou   +3 more
openaire   +2 more sources