Results 151 to 160 of about 933 (174)

RNA-seq profiles from grass carp tissues after reovirus (GCRV) infection based on singular and modular enrichment analyses

Molecular Immunology, 2014
Hemorrhagic disease of the grass carp, Ctenopharyngodon idella, is a fatal disease in fingerlings and yearlings caused by a reovirus, GCRV. RNA-seq data from four diseased grass carp tissues (gill, intestine, liver and spleen) were obtained at 2h before and six times after (2h, 24h, 48h, 72h, 96h and 120h) GCRV challenge.
Mijuan Shi, Rong Huang, Fukuan Du
exaly   +3 more sources

Comprehensive analysis of circRNA expression pattern and circRNA–mRNA–miRNA network in Ctenopharyngodon idellus kidney (CIK) cells after grass carp reovirus (GCRV) infection

Aquaculture, 2019
Abstract Grass carp reovirus (GCRV) causes grass carp hemorrhage disease and is one of the most damaging factors in the culture of grass carp (Ctenopharyngodon idellus). Until now, the mechanisms underlying GCRV infection have been largely unknown. To explore the molecular responses to GCRV infection in C.
Zi Liang, Yongjie Feng, Guangli Cao
exaly   +3 more sources

Grass Carp Reovirus (GCRV) infection activates the PERK-eIF2α pathway to promote the viral replication

Fish and Shellfish Immunology
Grass carp reovirus (GCRV) belongs to the genus Aquareovirus and is responsible for causing serious hemorrhagic disease in grass carp (Ctenopharyngodon idella), characterized by high mortality rates. Numerous animal viruses have been shown to activate endoplasmic reticulum stress (ERS).
Zeen Shen, Chengliang Gong
exaly   +3 more sources

Development of a novel candidate subunit vaccine against Grass carp reovirus Guangdong strain (GCRV-GD108)

Fish & Shellfish Immunology, 2013
Grass carp reovirus Guangdong 108 strain (GCRV-GD108) was recently isolated in Guangdong province, China. M6 gene of GCRV-GD108 was speculated encoding virus major outer capsid protein VP4. Blast analysis showed that the amino acid sequence of GCRV-GD108 VP4 was homologous to the structural protein VP4 of known Aquareoviruses (27.3-32.9 ...
Yuanyuan, Tian, Xing, Ye, Lili, Zhang, Guocheng, Deng, Yueqiang, Bai   +4 more
openaire   +2 more sources

Grass carp superoxide dismutases exert antioxidant function and inhibit autophagy to promote grass carp reovirus (GCRV) replication

International Journal of Biological Macromolecules
Superoxide dismutases (SODs) are potent antioxidants crucial for neutralizing reactive oxygen species (ROS) and protecting organisms from oxidative damage. In this study, we successfully cloned and analyzed two SOD genes, CiSOD1 and CiSOD2, from grass carp (Ctenopharyngodon idellus).
Lanjie Liao
exaly   +3 more sources

Molecular cloning of grass carp (Ctenopharyngodon idellus) T-bet and GATA-3, and their expression profiles with IFN-γ in response to grass carp reovirus (GCRV) infection

Fish Physiology and Biochemistry, 2012
Both T-bet and GATA-3, Th1/Th2 lineage-specific transcription factors, play important roles in the development of T cells and Th1/Th2 differentiation. In this study, T-bet and GATA-3 genes were cloned from grass carp (Ctenopharyngodon idellus). The putative primary structure of the polypeptide deduced from the cDNA sequence of grass carp T-bet ...
Lu, Wang, Na, Shang, Hong, Feng, Qionglin, Guo, Heping, Dai   +4 more
openaire   +2 more sources

A one‐step molecular biology method for simple and rapid detection of grass carp Ctenopharyngodon idella reovirus (GCRV) HZ08 strain

Journal of Fish Biology, 2013
Six reverse‐transcription loop‐mediated isothermal amplification (RT‐LAMP) primers designed against conserved regions of segment 6 (s6) gene were used for the detection of grass carp Ctenopharyngodon idella reovirus (GCRV) HZ08 subtype. The entire amplification could be completed within 40 min at 62·3° C.
W W, Zeng, Q, Wang, Y Y, Wang, D H, Xu, S Q, Wu   +4 more
openaire   +2 more sources

In vitro immunocompetence of two compounds isolated from Polygala tenuifolia and development of resistance against grass carp reovirus (GCRV) and Dactylogyrus intermedius in respective host

Fish & Shellfish Immunology, 2014
The present study was undertaken to isolate some compounds from methanol extract of Polygala tenuifolia and evaluate their immunostimulatory properties and antiviral activity using grass carp Ctenopharyngodon idella kidney (CIK) cells and GCRV. By applying insecticidal bioassay-guided, chromatography techniques and successive recrystallization, two ...
Xiao-Bo, Yu, Guang-Lu, Liu, Bin, Zhu, Kai, Hao, Fei, Ling, Gao-Xue, Wang   +5 more
openaire   +2 more sources

Development of a VP38 recombinant protein‐based indirect ELISA for detection of antibodies against grass carp reovirus genotype II (iELISA for detection of antibodies against GCRV II)

Journal of Fish Diseases, 2018
AbstractCurrently, serological assays for grass carp reovirus genotype II (GCRV‐II) diagnosis are not available. In this study, an indirect enzyme‐linked immunosorbent assay (ELISA) for the detection of antibodies against GCRV‐II was developed. The structural protein VP38 of GCRV‐II was used as the coating antigen.
Weiwei Zeng, Yingying Wang, Yanmin Guo, Sven M. Bergmann, Jiyuan Yin, Yingying Li, Yan Ren, Cunbin Shi, Qing Wang   +8 more
openaire   +2 more sources

Oral immunization with recombinant L. lactis expressing GCRV-II VP4 produces protection against grass carp reovirus infection

Fish & Shellfish Immunology
The hemorrhagic disease causing by grass carp reovirus (GCRV) infection, is associated with major economic losses and significant impact on aquaculture worldwide. VP4 of GCRV is one of the major outer capsid proteins which can induce an immune response in the host. In this study, pNZ8148-VP4/L.
Huiliang Wu, Jiyuan Yin, Siming Li, Hao Wang, Peng Jiang, Pengfei Li, Zhaoyang Ding, Han Yan, Bo Chen, Linchuan Wang, Qing Wang   +10 more
openaire   +2 more sources