Results 131 to 140 of about 756 (154)

The heat-labile toxin B subunit of E. coli fused with VP6 from GCRV (Grass carp reovirus) was expressed and folded into an active protein in rice calli

Protein Expression and Purification, 2022
Grass carp reovirus (GCRV) is one of the most serious pathogens threatening grass carp (Ctenopharyngon idellus) production in China. VP6 could be suitable for developing vaccine for the control of GCRV. Transgenic plants are an attractive bioreactor for their safety and ability to make economical vaccines.
Shanshan, Jin, Tongtong, Wang, Yan, Zhao, Xue, Liu, Yichen, Wang, Lingchuan, Jiang, Qiusheng, Zhang   +6 more
exaly   +3 more sources

B subunit of cholera toxin fused with VP7 from GCRV (grass carp reovirus) was expressed in E. coli and folds into an active protein

International Journal of Biological Macromolecules, 2020
Grass carp reovirus (GCRV) is one of the most serious pathogens threatening grass carp (Ctenopharyngodon idella) production and results in high mortality in China. To obtain a genetically engineered oral vaccine against GCRV, the cholera toxin B subunit (CTB) of Vibrio cholerae was fused to VP7 (CTB-VP7) and transformed into BL21(DE3) for expression ...
Yan, Zhao, Binglian, Xu, Borun, Zhu, Xue, Liu, Dongchun, Yan, Qiusheng, Zhang   +5 more
exaly   +3 more sources

Expression and active testing of VP7 from GCRV (Grass carp reovirus) fused with cholera toxin B subunit in rice calli

Protein Expression and Purification, 2019
Grass carp reovirus (GCRV) is one of the most serious pathogens threatening grass carp (Ctenopharyngodon idellus) production and results in high mortality in China. VP7 from GCRV is involved in viral infection and could be suitable for developing vaccines for the control of GCRV infection.
Qiusheng, Zhang, Binglian, Xu, Jiajia, Pan, Danyang, Liu, Ruoxian, Lv, Dongchun, Yan   +5 more
exaly   +3 more sources

Grass Carp Reovirus (GCRV) infection activates the PERK-eIF2α pathway to promote the viral replication

Fish & Shellfish Immunology
Grass carp reovirus (GCRV) belongs to the genus Aquareovirus and is responsible for causing serious hemorrhagic disease in grass carp (Ctenopharyngodon idella), characterized by high mortality rates. Numerous animal viruses have been shown to activate endoplasmic reticulum stress (ERS).
Zeen Shen, Yanling Qi, Wenbin Yu, Song Li, Zhuo Liu, Liuyang Li, Min Zhu, Chengliang Gong, Xiaolong Hu   +8 more
openaire   +2 more sources

An immersion challenge model for type II grass carp reovirus (GCRV-II) induces effective infection and upregulates antiviral immune response in grass carp

Fish & Shellfish Immunology
Grass carp is the most produced freshwater aquaculture species in China. However, high-density farming is often accompanied by severe disease outbreaks, causing significant economic losses to the industry. To support disease research and control, we developed an immersion challenge model in which fish were exposed to type II grass carp reovirus (4.23 ×
Guanyu Chen, Maolin Lv, Yuezong Xu, Yi Wang, Shijie Wang, Yizhong Lu, Sai Zeng, Jianguo Su   +7 more
openaire   +1 more source

A one‐step molecular biology method for simple and rapid detection of grass carp Ctenopharyngodon idella reovirus (GCRV) HZ08 strain

Journal of Fish Biology, 2013
Six reverse‐transcription loop‐mediated isothermal amplification (RT‐LAMP) primers designed against conserved regions of segment 6 (s6) gene were used for the detection of grass carp Ctenopharyngodon idella reovirus (GCRV) HZ08 subtype. The entire amplification could be completed within 40 min at 62·3° C.
W W, Zeng, Q, Wang, Y Y, Wang, D H, Xu, S Q, Wu   +4 more
openaire   +2 more sources

In vitro immunocompetence of two compounds isolated from Polygala tenuifolia and development of resistance against grass carp reovirus (GCRV) and Dactylogyrus intermedius in respective host

Fish & Shellfish Immunology, 2014
The present study was undertaken to isolate some compounds from methanol extract of Polygala tenuifolia and evaluate their immunostimulatory properties and antiviral activity using grass carp Ctenopharyngodon idella kidney (CIK) cells and GCRV. By applying insecticidal bioassay-guided, chromatography techniques and successive recrystallization, two ...
Xiao-Bo, Yu, Guang-Lu, Liu, Bin, Zhu, Kai, Hao, Fei, Ling, Gao-Xue, Wang   +5 more
openaire   +2 more sources

Development of a VP38 recombinant protein‐based indirect ELISA for detection of antibodies against grass carp reovirus genotype II (iELISA for detection of antibodies against GCRV II)

Journal of Fish Diseases, 2018
AbstractCurrently, serological assays for grass carp reovirus genotype II (GCRV‐II) diagnosis are not available. In this study, an indirect enzyme‐linked immunosorbent assay (ELISA) for the detection of antibodies against GCRV‐II was developed. The structural protein VP38 of GCRV‐II was used as the coating antigen.
Weiwei Zeng, Yingying Wang, Yanmin Guo, Sven M. Bergmann, Jiyuan Yin, Yingying Li, Yan Ren, Cunbin Shi, Qing Wang   +8 more
openaire   +2 more sources

Oral immunization with recombinant L. lactis expressing GCRV-II VP4 produces protection against grass carp reovirus infection

Fish & Shellfish Immunology
The hemorrhagic disease causing by grass carp reovirus (GCRV) infection, is associated with major economic losses and significant impact on aquaculture worldwide. VP4 of GCRV is one of the major outer capsid proteins which can induce an immune response in the host. In this study, pNZ8148-VP4/L.
Huiliang Wu, Jiyuan Yin, Siming Li, Hao Wang, Peng Jiang, Pengfei Li, Zhaoyang Ding, Han Yan, Bo Chen, Linchuan Wang, Qing Wang   +10 more
openaire   +2 more sources

SUSCEPTIBILITY TO GRASS CARP REOVIRUS (GCRV) OF SEVERAL FISH CELL LINES

Acta Hydrobiologica Sinica, 1985
Chuxia Deng, Xingqi Yang, Hongxi Chen
openaire   +1 more source