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Complete nucleotide sequence of the S10 genome segment of grass carp reovirus (GCRV) [PDF]

Diseases of Aquatic Organisms, 2001
Hemorrhagic disease, caused by the grass carp reovirus (GCRV), is one of the major diseases of grass carp in China. Little is known about the structure and function of the gene segments of this reovirus. The S10 genome segment of GCRV was cloned and the complete nucleotide sequence is reported here.
Zuo-Yan Zhu
exaly   +4 more sources

RNA-seq profiles from grass carp tissues after reovirus (GCRV) infection based on singular and modular enrichment analyses

Molecular Immunology, 2014
Hemorrhagic disease of the grass carp, Ctenopharyngodon idella, is a fatal disease in fingerlings and yearlings caused by a reovirus, GCRV. RNA-seq data from four diseased grass carp tissues (gill, intestine, liver and spleen) were obtained at 2h before and six times after (2h, 24h, 48h, 72h, 96h and 120h) GCRV challenge.
Mijuan Shi, Rong Huang, Fukuan Du
exaly   +4 more sources

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The heat-labile toxin B subunit of E. coli fused with VP6 from GCRV (Grass carp reovirus) was expressed and folded into an active protein in rice calli

Protein Expression and Purification, 2022
Grass carp reovirus (GCRV) is one of the most serious pathogens threatening grass carp (Ctenopharyngon idellus) production in China. VP6 could be suitable for developing vaccine for the control of GCRV. Transgenic plants are an attractive bioreactor for their safety and ability to make economical vaccines.
Qiusheng Zhang
exaly   +3 more sources

B subunit of cholera toxin fused with VP7 from GCRV (grass carp reovirus) was expressed in E. coli and folds into an active protein

International Journal of Biological Macromolecules, 2020
Grass carp reovirus (GCRV) is one of the most serious pathogens threatening grass carp (Ctenopharyngodon idella) production and results in high mortality in China. To obtain a genetically engineered oral vaccine against GCRV, the cholera toxin B subunit (CTB) of Vibrio cholerae was fused to VP7 (CTB-VP7) and transformed into BL21(DE3) for expression ...
Qiusheng Zhang
exaly   +3 more sources

Detection of grass carp reovirus (GCRV) with monoclonal antibodies

Archives of Virology, 2013
Grass carp reovirus (GCRV) is a pathogen that causes hemorrhagic disease of grass carp. It is the most serious infectious disease of carp and causes serious losses of fingerlings of grass carp and black carp. In this study, a recombinant VP4, one of the viral core proteins, was constructed with a histidine tag and expressed at a high level in E.
Jing, Hongli, Zhang, Lifeng, Fang, Zhenzhen, Xu, Lipu, Zhang, Min, Wang, Na, Jiang, Yulin, Lin, Xiangmei   +7 more
openaire   +2 more sources

Cloning of six serpin genes and their responses to GCRV infection in grass carp (Ctenopharyngodon idella)

Fish and Shellfish Immunology, 2019
Grass carp, an economically important aquaculture fish, is very sensitive to Grass Carp Reovirus (GCRV). Haemorrhagic disease caused by GCRV infection can cause large-scale death of first-year grass carp, thereby severely restricting the intensive ...
Liangming Chen, Rong Huang, Denghui Zhu   +2 more
exaly   +2 more sources

Expression and active testing of VP7 from GCRV (Grass carp reovirus) fused with cholera toxin B subunit in rice calli

Protein Expression and Purification, 2019
Grass carp reovirus (GCRV) is one of the most serious pathogens threatening grass carp (Ctenopharyngodon idellus) production and results in high mortality in China. VP7 from GCRV is involved in viral infection and could be suitable for developing vaccines for the control of GCRV infection.
Qiusheng Zhang
exaly   +3 more sources