Pressure and temperature dependence of fluorescence anisotropy of Green Fluorescent Protein [PDF]
arXiv, 2022 We have studied the effect of high hydrostatic pressure and temperature on the steady state fluorescence anisotropy of Green Fluorescent Protein (GFP). We find that the fluorescence anisotropy of GFP at a constant temperature decreases with increasing pressure.
Kaur H, Nguyen K, Kumar P.
arxiv +2 more sources
Direction dependent mechanical unfolding and Green Fluorescent Protein as a force sensor [PDF]
Physical Review E 84, 021918 (2011), 2011 An Ising--like model of proteins is used to investigate the mechanical unfolding of the Green Fluorescent Protein along different directions. When the protein is pulled from its ends, we recover the major and minor unfolding pathways observed in experiments.
arxiv +1 more source
Ultralow-threshold green fluorescent protein laser based on high Q microbubble resonators [PDF]
, 2022 Biological lasers have attracted vast attention because of their potential medical application prospects, especially the low threshold biological laser, which can be used for ultrasensitive biological detection while ensuring that its luminous gain medium is not damaged by the high-energy pump light.
arxiv +1 more source
Ultraviolet optical horn antennas for label-free detection of single proteins [PDF]
Nature Communications, Nature Publishing Group, 2022, 13, pp.1842, 2022 Single-molecule fluorescence techniques have revolutionized our ability to study proteins. However, the presence of a fluorescent label can alter the protein structure and/or modify its reaction with other species. To avoid the need for a fluorescent label, the intrinsic autofluorescence of proteins in the ultraviolet offers the benefits of ...
arxiv +1 more source
Combining single-molecule super-resolved localization microscopy with fluorescence polarization imaging to study cellular processes [PDF]
2021 J. Phys. Photonics 3 034010, 2020 Super-resolution microscopy has catalyzed valuable insights into the sub-cellular, mechanistic details of many different biological processes across a wide range of cell types. Fluorescence polarization spectroscopy tools have also enabled important insights into cellular processes through identifying orientational changes of biological molecules ...
arxiv +1 more source
Dynamical Decoherence and Memory Effects in Green Fluorescent Proteins by Dielectric Relaxation [PDF]
arXiv, 2022 In this article, we explore the dynamical decoherence of the chromophores within a green fluorescent protein when coupled to a finite-temperature dielectric environment. Such systems are of significant interest due to their anomalously long coherence lifetimes compared to other biomolecules.
arxiv
Nonequilibrium dynamics of an exactly solvable Ising-like model and protein translocation [PDF]
Europhysics Letters 102, 10001 (2013), 2013 Using an Ising-like model of protein mechanical unfolding, we introduce a diffusive dynamics on its exactly known free energy profile, reducing the nonequilibrium dynamics of the model to a biased random walk. As an illustration, the model is then applied to the protein translocation phenomenon, taking inspiration from a recent experiment on the green ...
arxiv +1 more source
Infinite average lifetime of an unstable bright state in the green fluorescent protein [PDF]
, 2005 The time evolution of the fluorescence intensity emitted by well-defined ensembles of Green Fluorescent Proteins has been studied by using a standard confocal microscope. In contrast with previous results obtained in single molecule experiments, the photo-bleaching of the ensemble is well described by a model based on Levy statistics.
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A Diabatic Three-State Representation of Photoisomerization in the Green Fluorescent Protein Chromophore [PDF]
S. Olsen and R.H. McKenzie, The Journal of Chemical Physics, 130,
184302 (2009), 2009 We give a quantum chemical description of bridge photoisomerization reaction of green fluorescent protein (GFP) chromophores using a representation over three diabatic states. Bridge photoisomerization leads to non-radiative decay, and competes with fluorescence in these systems.
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Visible Light-Activated Photosensitization of Hybridization of Far-red Fluorescent Protein and Silk [PDF]
, 2018 Fluorescent proteins often result in phototoxicity and cytotoxicity, in particular because some red fluorescent proteins (RFP) produce and release reactive oxygen species (ROS). The photogeneration of ROS is considered as a detrimental side effect in cellular imaging or is proactively utilized for ablating cancerous tissue.
arxiv +1 more source