Results 21 to 30 of about 568,395 (338)

Improved blue, green, and red fluorescent protein tagging vectors for S. cerevisiae. [PDF]

PLoS ONE, 2013
Fluorescent protein fusions are a powerful tool to monitor the localization and trafficking of proteins. Such studies are particularly easy to carry out in the budding yeast Saccharomyces cerevisiae due to the ease with which tags can be introduced into ...
Sidae Lee, Wendell A Lim, Kurt S Thorn
doaj   +1 more source

Imaging the Environment of Green Fluorescent Protein [PDF]

Biophysical Journal, 2002
An emerging theme in cell biology is that cell surface receptors need to be considered as part of supramolecular complexes of proteins and lipids facilitating specific receptor conformations and distinct distributions, e.g., at the immunological synapse. Thus, a new goal is to develop bioimaging that not only locates proteins in live cells but can also
Suhling, K, Siegel, J, Phillips, D, Davis, D M, LevequeFort, S, Webb, S E D, French, P M W   +6 more
openaire   +5 more sources

Photochromicity and Fluorescence Lifetimes of Green Fluorescent Protein [PDF]

The Journal of Physical Chemistry B, 1999
The green fluorescent protein (GFP) of the bioluminescent jellyfish Aequorea and its mutants have gained widespread usage as an indicator of structure and function within cells. Proton transfer has been implicated in the complex photophysics of the wild-type molecule, exhibiting a protonated A species excited at 400 nm, and two deprotonated excited ...
Striker, G., Subramaniam, V., Seidel, C., Volkmer, A.   +3 more
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Green Fluorescent Protein-Mediated Biomineralization of Silver Microparticles

Orbital: The Electronic Journal of Chemistry, 2021
Biomineralization is a bio-inspired technique of creating inorganic nanostructures using peptides or proteins. An important consideration in selecting a biomineralization agent is the overall shape or geometry of the protein since this can influence the ...
Ma. Monica Cabiles, Brandon Cyril Lira, Jose Isagani Janairo   +2 more
doaj   +3 more sources

Variants of Green Fluorescent Protein GFPxm [PDF]

Marine Biotechnology, 2006
As research progresses, fluorescent proteins useful for optical marking will evolve toward brighter, monomeric forms that are more diverse in color. We previously reported a new fluorescent protein from Aequorea macrodactyla, GFPxm, that exhibited many characteristics similar to wild-type green fluorescent protein (GFP).
Luo, W. X., Cheng, T., Guan, B. Q., Li, S. W., Miao, J., Zhang, J., Xia, N. S.   +6 more
openaire   +3 more sources

Using photoactivatable fluorescent protein Dendra2 to track protein movement

BioTechniques, 2007
Photoactivatable fluorescent proteins are capable of dramatic changes in fluorescent properties in response to specific light irradiation. For example, they can be converted from cyan to green, or from green to red, or from nonfluorescent to a brightly ...
Dmitriy M. Chudakov, Sergey Lukyanov, Konstantin A. Lukyanov   +2 more
doaj   +1 more source

Evolution of a fluorinated green fluorescent protein [PDF]

Proceedings of the National Academy of Sciences, 2007
The fluorescence of bacterial cells expressing a variant (GFPm) of the green fluorescent protein (GFP) was reduced to background levels by global replacement of the leucine residues of GFPm by 5,5,5-trifluoroleucine. Eleven rounds of random mutagenesis and screening via fluorescence-activated cell sorting yielded a GFP mutant containing 20
Yoo, Tae-Hyeon, Link, A. James, Tirrell, David A.   +2 more
openaire   +5 more sources

Two-Color Green Fluorescent Protein Time-Lapse Imaging

BioTechniques, 1998
The Aequorea victoria green fluorescent protein (GFP) is widely recognized as a powerful tool in cell biology, serving as a vital reporter for monitoring localization and dynamics of intracellular proteins and organelles over time.
Jan Ellenberg, Jennifer Lippincott-Schwartz, John F. Presley   +2 more
doaj   +1 more source

Effects of cellular prion protein on rapid eye movement sleep deprivation-induced spatial memory impairment

Journal of Integrative Neuroscience, 2019
The effects of cellular prion protein on rapid eye movement sleep deprivation-induced spatial memory impairment were investigated, and the related mechanisms explored.
Li Hu, Peng Li, Zhendong You, Zhaohuan Zhang, Hongyang Jin, Liuqing Huang   +5 more
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Aequorea's secrets revealed: New fluorescent proteins with unique properties for bioimaging and biosensing.

PLoS Biology, 2020
Using mRNA sequencing and de novo transcriptome assembly, we identified, cloned, and characterized 9 previously undiscovered fluorescent protein (FP) homologs from Aequorea victoria and a related Aequorea species, with most sequences highly divergent ...
Gerard G Lambert, Hadrien Depernet, Guillaume Gotthard, Darrin T Schultz, Isabelle Navizet, Talley Lambert, Stephen R Adams, Albertina Torreblanca-Zanca, Meihua Chu, Daphne S Bindels, Vincent Levesque, Jennifer Nero Moffatt, Anya Salih, Antoine Royant, Nathan C Shaner   +14 more
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