Results 21 to 30 of about 1,124,962 (295)
Improved split fluorescent proteins for endogenous protein labeling. [PDF]
, 2017 Self-complementing split fluorescent proteins (FPs) have been widely used for protein labeling, visualization of subcellular protein localization, and detection of cell-cell contact.
Feng, Siyu +5 more
core +2 more sources
Two-Color Green Fluorescent Protein Time-Lapse Imaging
BioTechniques, 1998 The Aequorea victoria green fluorescent protein (GFP) is widely recognized as a powerful tool in cell biology, serving as a vital reporter for monitoring localization and dynamics of intracellular proteins and organelles over time.
Jan Ellenberg +2 more
doaj +1 more source
Photoactivation of green fluorescent protein [PDF]
Current Biology, 1997 Considerable insight into many cell biological processes can be obtained by following the turnover of individual protein species in time and space. In living cells, this has been achieved by following fluorescence recovery after local microbeam photobleaching of microinjected fluorescently labelled protein (see, for example, [1xSpindle microtubule ...
Sawin, Kenneth E., Nurse, Paul
openaire +2 more sources
CemOrange2 fusions facilitate multifluorophore subcellular imaging in C. elegans [PDF]
, 2019 Due to its ease of genetic manipulation and transparency, Caenorhabditis elegans (C. elegans) has become a preferred model system to study gene function by microscopy.
Buland, Justin R +14 more
core +3 more sources
Electrochemical fluorescence switching of enhanced green fluorescent protein
Biosensors and Bioelectronics, 2023 Switchable fluorescent proteins, for which fluorescence can be switched ON and OFF, are widely used for molecule tracking and super resolution imaging. However, the robust use of the switchable fluorescent proteins is still limited as either the switching is not repeatable, or such switching requires irradiation with coupled lasers of different ...
Yang, Y +8 more
openaire +3 more sources
Direction dependent mechanical unfolding and Green Fluorescent Protein as a force sensor [PDF]
, 2011 An Ising--like model of proteins is used to investigate the mechanical unfolding of the Green Fluorescent Protein along different directions. When the protein is pulled from its ends, we recover the major and minor unfolding pathways observed in ...
Caraglio, M. +2 more
core +2 more sources
PLoS Biology, 2020 Using mRNA sequencing and de novo transcriptome assembly, we identified, cloned, and characterized 9 previously undiscovered fluorescent protein (FP) homologs from Aequorea victoria and a related Aequorea species, with most sequences highly divergent ...
Gerard G Lambert +14 more
doaj +1 more source
Imaging individual green fluorescent proteins [PDF]
Nature, 1997 Recent advances in fluorescence microscopy techniques have allowed the video-time imaging of single molecules of fluorescent dyes covalently bound to proteins in aqueous environments1. However, the techniques have not been exploited fully because proteins can be difficult to label, and dye modification may cause partial or complete loss of activity ...
D W, Pierce, N, Hom-Booher, R D, Vale
openaire +2 more sources
The coherent dynamics of photoexcited green fluorescent proteins [PDF]
, 2001 The coherent dynamics of vibronic wave packets in the green fluorescent protein is reported. At room temperature the non-stationary dynamics following impulsive photoexcitation displays an oscillating optical transmissivity pattern with components at 67 ...
Beltram, Fabio +8 more
core +3 more sources
Journal of Integrative Neuroscience, 2019 The effects of cellular prion protein on rapid eye movement sleep deprivation-induced spatial memory impairment were investigated, and the related mechanisms explored.
Li Hu +5 more
doaj +1 more source