Results 31 to 40 of about 1,124,962 (295)

Live-Cell Imaging of Single Receptor Composition Using Zero-Mode Waveguide Nanostructures [PDF]

, 2012
We exploit the optical and spatial features of subwavelength nanostructures to examine individual receptors on the plasma membrane of living cells. Receptors were sequestered in portions of the membrane projected into zero-mode waveguides.
Dougherty, Dennis A., Korlach, Jonas, Lester, Henry A., Luong, Khai, Richards, Christopher I., Srinivasan, Rahul, Turner, Stephen W.   +6 more
core   +1 more source

Use of Green Fluorescent Protein to Detect Expression of nif Genes of Azoarcus sp. BH72, a Grass-Associated Diazotroph, on Rice Roots

Molecular Plant-Microbe Interactions, 1998
A gfp (green fluorescent protein) cassette for transcriptional fusions has been developed to study gene expression in Azoarcus sp. BH72 in association with plant roots.
Tanja Egener, Thomas Hurek, Barbara Reinhold-Hurek   +2 more
doaj   +1 more source

Local fitness landscape of the green fluorescent protein

Nature, 2016
Fitness landscapes depict how genotypes manifest at the phenotypic level and form the basis of our understanding of many areas of biology, yet their properties remain elusive. Previous studies have analysed specific genes, often using their function as a
K. Sarkisyan, D. Bolotin, Margarita V. Meer, D. Usmanova, A. Mishin, G. Sharonov, D. Ivankov, N. Bozhanova, M. Baranov, Onuralp Soylemez, N. S. Bogatyreva, P. Vlasov, Evgeny S. Egorov, M. Logacheva, A. Kondrashov, D. Chudakov, E. Putintseva, I. Mamedov, Dan S. Tawfik, K. Lukyanov, F. Kondrashov   +20 more
semanticscholar   +1 more source

Bioconjugation of Green Fluorescent Protein via an Unexpectedly Stable Cyclic Sulfonium Intermediate [PDF]

, 2012
Bioconjugation of superfolder GFP involving the formation of an unusually stable, and unprecedented, cyclic sulfonium species is described. This sulfonium can undergo smooth reaction with a range of nucleophiles to give sulfur-, selenium- and azide ...
Caddick, S, Fitzmaurice, RJ, Moody, P, Nathani, R, Smith, MEB   +4 more
core   +1 more source

Histo-pathology study of the growth of Trichoderma harzianum, Phaeomoniella chlamydospora and Eutypa lata on grapevine pruning wounds

Phytopathologia Mediterranea, 2011
Protecting grapevine pruning wounds by inoculating them with Trichoderma spp. can preventinfection from trunk disease pathogens. The growth and interactions of both, the biological control agent Trichoderma spp.
Cheusi MUTAWILA, Paul FOURIE, Francois HALLEEN, Lizel MOSTERT   +3 more
doaj   +1 more source

Construction of Yersinia pestis Strain Producing Fluorescent Protein GFP and Prospects of Its Usage

Проблемы особо опасных инфекций, 2020
Objective of the study was to construct the recombinant Y. pestis strain producing fluorescent protein GFP and analysis of the prospects of its usage for investigation of interaction of the agent with the protozoa, and also macroorganism of mammals ...
L. M. Kukleva, I. V. Tuchkov, E. G. Oglodin, Z. L. Devdariani, O. A. Morozov, O. S. Kuznetsov, V. G. Germanchuk, G. A. Eroshenko   +7 more
doaj   +1 more source

Incomplete proteasomal degradation of green fluorescent proteins in the context of tandem fluorescent protein timers

bioRxiv, 2015
Tandem fluorescent protein timers (tFTs) report on protein age through time-dependent change in color, which can be exploited to study protein turnover and trafficking.
Anton Khmelinskii, M. Meurer, Chi‐ting Ho, B. Besenbeck, Julia Füller, M. K. Lemberg, B. Bukau, A. Mogk, M. Knop   +8 more
semanticscholar   +1 more source

The Yersinia enterocolitica Ysa type III secretion system is expressed during infections both in vitro and in vivo. [PDF]

, 2013
Yersinia enterocolitica biovar 1B maintains two type III secretion systems (T3SS) that are involved in pathogenesis, the plasmid encoded Ysc T3SS and the chromosomally encoded Ysa T3SS.
Bent, Zachary W, Branda, Steven S, Young, Glenn M   +2 more
core   +1 more source

A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum

Nature Methods, 2013
We report a monomeric yellow-green fluorescent protein, mNeonGreen, derived from a tetrameric fluorescent protein from the cephalochordate Branchiostoma lanceolatum.
Nathan Christopher Shaner, G. G. Lambert, Andrew Chammas, Yuhui Ni, Paula J. Cranfill, M. A. Baird, Brittney R. Sell, John R. Allen, R. Day, M. Israelsson, M. Davidson, Jiwu Wang   +11 more
semanticscholar   +1 more source

Applications of Green Fluorescent Protein in Plants

BioTechniques, 1997
Green fluorescent protein (GFP) is increasingly being used in plant biology from the cellular level to whole plant level. At the cellular level, GFP is being used as an in vivo reporter to assess frequency of transient and stable transformation.
Staci M. Leffel, Stephen A. Mabon, C. Neal Stewart   +2 more
doaj   +1 more source