Results 31 to 40 of about 910,722 (206)

Applications of Green Fluorescent Protein in Plants

BioTechniques, 1997
Green fluorescent protein (GFP) is increasingly being used in plant biology from the cellular level to whole plant level. At the cellular level, GFP is being used as an in vivo reporter to assess frequency of transient and stable transformation.
Staci M. Leffel, Stephen A. Mabon, C. Neal Stewart   +2 more
doaj   +1 more source

Photochromicity and Fluorescence Lifetimes of Green Fluorescent Protein [PDF]

The Journal of Physical Chemistry B, 1999
The green fluorescent protein (GFP) of the bioluminescent jellyfish Aequorea and its mutants have gained widespread usage as an indicator of structure and function within cells. Proton transfer has been implicated in the complex photophysics of the wild-type molecule, exhibiting a protonated A species excited at 400 nm, and two deprotonated excited ...
Striker, G., Subramaniam, V., Seidel, C., Volkmer, A.   +3 more
openaire   +2 more sources

Improved blue, green, and red fluorescent protein tagging vectors for S. cerevisiae.

PLoS ONE, 2013
Fluorescent protein fusions are a powerful tool to monitor the localization and trafficking of proteins. Such studies are particularly easy to carry out in the budding yeast Saccharomyces cerevisiae due to the ease with which tags can be introduced into ...
Sidae Lee, Wendell A Lim, Kurt S Thorn, Kurt S Thorn   +3 more
doaj   +1 more source

Herpes Simplex Virus Thymidine Kinase-Green Fluorescent Protein Fusion Gene: New Tool for Gene Transfer Studies and Gene Therapy

BioTechniques, 1998
Green fluorescent protein (GFP) and herpes simplex virus type-1 thymidine kinase (TK) are commonly used markers in gene transfer studies. The latter gene has also proven to be an effective tool in cancer “suicide” gene therapy.
Sami Loimas, Jarmo Wahlfors, Juhani Jänne   +2 more
doaj   +1 more source

Implementation of a Practical Teaching Course on Protein Engineering

Biology, 2022
Protein Engineering is a highly evolved field of engineering aimed at developing proteins for specific industrial, medical, and research applications.
Luciana C. Gomes, Carla Ferreira, Filipe J. Mergulhão   +2 more
doaj   +1 more source

Simultaneous Real-Time Detection of Initiator- and Effector-Caspase Activation by Double Fluorescence Resonance Energy Transfer Analysis

Journal of Pharmacological Sciences, 2005
Fluorescence resonance energy transfer (FRET) with green fluorescent protein (GFP) variants has become widely used for biochemical research. In order to expand the choice of fluorescent range in FRET analysis, we designed various color versions of the ...
Hiroshi Kawai, Takuo Suzuki, Tetsu Kobayashi, Haruna Sakurai, Hisayuki Ohata, Kazuo Honda, Kazutaka Momose, Iyuki Namekata, Hikaru Tanaka, Koki Shigenobu, Ryu Nakamura, Takao Hayakawa, Toru Kawanishi   +12 more
doaj   +1 more source

Adenoviral targeting of malignant melanoma for fluorescence-guided surgery prevents recurrence in orthotopic nude-mouse models. [PDF]

, 2015
Malignant melanoma requires precise resection in order to avoid metastatic recurrence. We report here that the telomerase-dependent, green fluorescent protein (GFP)-containing adenovirus OBP-401 could label malignant melanoma with GFP in situ in ...
Bouvet, Michael, Fujiwara, Toshiyoshi, Hoffman, Robert M, Kagawa, Shunsuke, Kishimoto, Hiroyuki, Takehara, Kiyoto, Urata, Yasuo, Yano, Shuya   +7 more
core   +1 more source

Fluorescent protein markers to tag collagenous proteins: The paradigm of procollagen VII [PDF]

, 2009
Fluorescent proteins are powerful markers allowing tracking expression, intracellular localization, and translocation of tagged proteins but their effects on the structure and assembly of complex extracellular matrix proteins has not been investigated ...
Chung, Hye J., Fertala, Andrzej, Steplewski, Andrzej, Uitto, Jouni   +3 more
core   +2 more sources

Photoactivatable Green Fluorescent Protein-Tubulin [PDF]

, 2010
Direct observations of live cells expressing fluorescently tagged tubulin have led to important advances in our understanding of mitosis. A limitation of this approach is that all of the cells' microtubules are fluorescent and thus observation of the behavior of specific subsets of microtubules is precluded.
U Serdar, Tulu, Nick P, Ferenz, Patricia, Wadsworth   +2 more
openaire   +2 more sources

Generation of a human embryonic stem cell line expressing tetrameric Zoanthus sp. green fluorescent protein: NERCe002-A-1

Stem Cell Research, 2018
The human embryonic stem cell (hESC) line NERCe002-A-1 was generated through lentiviral transduction of the original NERCe002-A-1 hESC line with Zoanthus sp. green fluorescent protein (ZsGreen). Cells that expressed ZsGreen showed a >8.6-fold increase in
Xingxiang Duan, Menghan Xie, Yingying Peng, Liang Hu, Juan Yu, Sicong Zeng, Yang Wang, Guangxiu Lu, Ge Lin, Yi Sun   +9 more
doaj   +1 more source