Photochromicity and Fluorescence Lifetimes of Green Fluorescent Protein [PDF]
The Journal of Physical Chemistry B, 1999 The green fluorescent protein (GFP) of the bioluminescent jellyfish Aequorea and its mutants have gained widespread usage as an indicator of structure and function within cells. Proton transfer has been implicated in the complex photophysics of the wild-type molecule, exhibiting a protonated A species excited at 400 nm, and two deprotonated excited ...
Striker, G. +3 more
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Fluorescent Protein Based FRET Pairs with Improved Dynamic Range for Fluorescence Lifetime Measurements. [PDF]
PLoS ONE, 2015 Fluorescence Resonance Energy Transfer (FRET) using fluorescent protein variants is widely used to study biochemical processes in living cells. FRET detection by fluorescence lifetime measurements is the most direct and robust method to measure FRET. The
Bobin George Abraham +5 more
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Implementation of a Practical Teaching Course on Protein Engineering
Biology, 2022 Protein Engineering is a highly evolved field of engineering aimed at developing proteins for specific industrial, medical, and research applications.
Luciana C. Gomes +2 more
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Neurofilament high molecular weight-green fluorescent protein fusion is normally expressed in neurons and transported in axons: a neuronal marker to investigate the biology of neurofilaments [PDF]
, 2006 The carboxy-terminal side arm of the neurofilament high subunit consists of a highly phosphorylated domain and a negatively charged region. Multiple evidences suggested that these domains are essential for the axonal phosphorylation and transport of ...
A. Barthelaix +6 more
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Journal of Pharmacological Sciences, 2005 Fluorescence resonance energy transfer (FRET) with green fluorescent protein (GFP) variants has become widely used for biochemical research. In order to expand the choice of fluorescent range in FRET analysis, we designed various color versions of the ...
Hiroshi Kawai +12 more
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Fluorescent protein markers to tag collagenous proteins: The paradigm of procollagen VII [PDF]
, 2009 Fluorescent proteins are powerful markers allowing tracking expression, intracellular localization, and translocation of tagged proteins but their effects on the structure and assembly of complex extracellular matrix proteins has not been investigated ...
Chung, Hye J. +3 more
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Photoactivatable Green Fluorescent Protein-Tubulin [PDF]
, 2010 Direct observations of live cells expressing fluorescently tagged tubulin have led to important advances in our understanding of mitosis. A limitation of this approach is that all of the cells' microtubules are fluorescent and thus observation of the behavior of specific subsets of microtubules is precluded.
U Serdar, Tulu +2 more
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CD81 and claudin 1 coreceptor association: role in hepatitis C virus entry. [PDF]
, 2008 Hepatitis C virus (HCV) is an enveloped positive-stranded RNA hepatotropic virus. HCV pseudoparticles infect liver-derived cells, supporting a model in which liver-specific molecules define HCV internalization.
Balfe, Peter +12 more
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Stem Cell Research, 2018 The human embryonic stem cell (hESC) line NERCe002-A-1 was generated through lentiviral transduction of the original NERCe002-A-1 hESC line with Zoanthus sp. green fluorescent protein (ZsGreen). Cells that expressed ZsGreen showed a >8.6-fold increase in
Xingxiang Duan +9 more
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Adenoviral targeting of malignant melanoma for fluorescence-guided surgery prevents recurrence in orthotopic nude-mouse models. [PDF]
, 2015 Malignant melanoma requires precise resection in order to avoid metastatic recurrence. We report here that the telomerase-dependent, green fluorescent protein (GFP)-containing adenovirus OBP-401 could label malignant melanoma with GFP in situ in ...
Bouvet, Michael +7 more
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